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Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species

Anthikan Klomchit, Mark S Calabon, Sompradtana Worabandit, Jack A Weaver, Elfina Karima, Fabrizio Alberti, Claudio Greco Orcid Logo, Siraprapa Mahanil Orcid Logo

Journal of Applied Microbiology, Volume: 135, Issue: 5

Swansea University Authors: Elfina Karima, Claudio Greco Orcid Logo

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DOI (Published version): 10.1093/jambio/lxae114

Abstract

AimsNeocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phyl...

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Published in: Journal of Applied Microbiology
ISSN: 1365-2672
Published: Oxford University Press (OUP) 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa66499
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This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis.Methods and resultsDetailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-&#x3B1;), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17&#x2013;0253, MFLUCC 17&#x2013;0257, and MFLUCC 17&#x2013;0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17&#x2013;0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17&#x2013;0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17&#x2013;0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters.ConclusionTwo fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. 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Greco was supported by the BBSRC (BB/V005723/2). E. Karima&#x2019;s PhD Scholarship was supported by EPSRC/Swansea University (EP/W524694/1). Jack Weaver was supported by a scholarship from the Engineering and Physical Sciences Re- search Council and the Biotechnology and Biological Sci- ences Research Council [EP/L016494/1] through the Centre for Doctoral Training in Synthetic Biology (SynBioCDT). Fab- rizio Alberti was supported by a UKRI Future Leaders Fellow- ship [MR/V022334/1]. Siraprapa Mahanil was supported by Mae Fah Luang University under the grant name &#x201C;Reinvent- ing University 2021.&#x201D;</funders><projectreference/><lastEdited>2024-06-13T12:23:51.6574022</lastEdited><Created>2024-05-23T11:57:17.8984570</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Anthikan</firstname><surname>Klomchit</surname><order>1</order></author><author><firstname>Mark S</firstname><surname>Calabon</surname><order>2</order></author><author><firstname>Sompradtana</firstname><surname>Worabandit</surname><order>3</order></author><author><firstname>Jack A</firstname><surname>Weaver</surname><order>4</order></author><author><firstname>Elfina</firstname><surname>Karima</surname><order>5</order></author><author><firstname>Fabrizio</firstname><surname>Alberti</surname><order>6</order></author><author><firstname>Claudio</firstname><surname>Greco</surname><orcid>0000-0003-3067-0999</orcid><order>7</order></author><author><firstname>Siraprapa</firstname><surname>Mahanil</surname><orcid>0000-0002-4314-6136</orcid><order>8</order></author></authors><documents><document><filename>66499__30456__1408ceec5cfc40b6ac1ae93bad290146.pdf</filename><originalFilename>66499.VoR.pdf</originalFilename><uploaded>2024-05-24T12:39:39.5792853</uploaded><type>Output</type><contentLength>1934236</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; The Author(s) 2024. 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spelling 2024-06-13T12:23:51.6574022 v2 66499 2024-05-23 Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species bde7b2231ab0c85d533a6b2578bdbfeb Elfina Karima Elfina Karima true false cacac6459bd7cf4a241f63661006036f 0000-0003-3067-0999 Claudio Greco Claudio Greco true false 2024-05-23 BGPS AimsNeocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis.Methods and resultsDetailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-α), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17–0253, MFLUCC 17–0257, and MFLUCC 17–0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17–0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17–0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17–0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters.ConclusionTwo fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. In addition, full genome sequencing and bioinformatics analysis of N. mangrovei MFLUCC 17–0253 were obtained. Journal Article Journal of Applied Microbiology 135 5 Oxford University Press (OUP) 1365-2672 one new taxon, bioactivity, mangrove fungi, multi-locus phylogenetic analyses, whole genome, biosynthetic gene clusters 9 5 2024 2024-05-09 10.1093/jambio/lxae114 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University SU Library paid the OA fee (TA Institutional Deal) C. Greco was supported by the BBSRC (BB/V005723/2). E. Karima’s PhD Scholarship was supported by EPSRC/Swansea University (EP/W524694/1). Jack Weaver was supported by a scholarship from the Engineering and Physical Sciences Re- search Council and the Biotechnology and Biological Sci- ences Research Council [EP/L016494/1] through the Centre for Doctoral Training in Synthetic Biology (SynBioCDT). Fab- rizio Alberti was supported by a UKRI Future Leaders Fellow- ship [MR/V022334/1]. Siraprapa Mahanil was supported by Mae Fah Luang University under the grant name “Reinvent- ing University 2021.” 2024-06-13T12:23:51.6574022 2024-05-23T11:57:17.8984570 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Anthikan Klomchit 1 Mark S Calabon 2 Sompradtana Worabandit 3 Jack A Weaver 4 Elfina Karima 5 Fabrizio Alberti 6 Claudio Greco 0000-0003-3067-0999 7 Siraprapa Mahanil 0000-0002-4314-6136 8 66499__30456__1408ceec5cfc40b6ac1ae93bad290146.pdf 66499.VoR.pdf 2024-05-24T12:39:39.5792853 Output 1934236 application/pdf Version of Record true © The Author(s) 2024. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. true eng https://creativecommons.org/licenses/by/4.0/
title Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
spellingShingle Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
Elfina Karima
Claudio Greco
title_short Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
title_full Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
title_fullStr Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
title_full_unstemmed Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
title_sort Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species
author_id_str_mv bde7b2231ab0c85d533a6b2578bdbfeb
cacac6459bd7cf4a241f63661006036f
author_id_fullname_str_mv bde7b2231ab0c85d533a6b2578bdbfeb_***_Elfina Karima
cacac6459bd7cf4a241f63661006036f_***_Claudio Greco
author Elfina Karima
Claudio Greco
author2 Anthikan Klomchit
Mark S Calabon
Sompradtana Worabandit
Jack A Weaver
Elfina Karima
Fabrizio Alberti
Claudio Greco
Siraprapa Mahanil
format Journal article
container_title Journal of Applied Microbiology
container_volume 135
container_issue 5
publishDate 2024
institution Swansea University
issn 1365-2672
doi_str_mv 10.1093/jambio/lxae114
publisher Oxford University Press (OUP)
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description AimsNeocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis.Methods and resultsDetailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-α), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17–0253, MFLUCC 17–0257, and MFLUCC 17–0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17–0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17–0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17–0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters.ConclusionTwo fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. In addition, full genome sequencing and bioinformatics analysis of N. mangrovei MFLUCC 17–0253 were obtained.
published_date 2024-05-09T20:44:02Z
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