No Cover Image

Journal article 229 views 36 downloads

Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers

Arjun Ajith Mohan Orcid Logo, Sudhaunsh Deshpande, Tom Dunlop Orcid Logo, Beth Norman, Daniela Oliviera, Georgeta Vulpe, Felismina Moreira Orcid Logo, Sanjiv Sharma Orcid Logo

Biosensors and Bioelectronics: X, Volume: 20, Start page: 100513

Swansea University Authors: Arjun Ajith Mohan Orcid Logo, Tom Dunlop Orcid Logo, Georgeta Vulpe, Sanjiv Sharma Orcid Logo

  • 66925.VoR.pdf

    PDF | Version of Record

    © 2024 The Author(s). This is an open access article under the CC BY license.

    Download (5.44MB)

Check full text

DOI (Published version): 10.1016/j.biosx.2024.100513

Abstract

Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI M...

Full description

Published in: Biosensors and Bioelectronics: X
ISSN: 2590-1370
Published: Elsevier BV 2024
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa66925
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2024-07-02T14:52:41Z
last_indexed 2024-07-02T14:52:41Z
id cronfa66925
recordtype SURis
fullrecord <?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>66925</id><entry>2024-07-02</entry><title>Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers</title><swanseaauthors><author><sid>852f42c2a4dd3f47711475ca17030bf9</sid><ORCID>0000-0001-6781-2108</ORCID><firstname>Arjun</firstname><surname>Ajith Mohan</surname><name>Arjun Ajith Mohan</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>809395460ab1e6b53a906b136d919c41</sid><ORCID>0000-0002-5851-8713</ORCID><firstname>Tom</firstname><surname>Dunlop</surname><name>Tom Dunlop</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>d1d5815c60d63534a10a81489255c681</sid><firstname>Georgeta</firstname><surname>Vulpe</surname><name>Georgeta Vulpe</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>b6b7506358522f607b171ec9c94757b7</sid><ORCID>0000-0003-3828-737X</ORCID><firstname>Sanjiv</firstname><surname>Sharma</surname><name>Sanjiv Sharma</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2024-07-02</date><deptcode>EAAS</deptcode><abstract>Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (~32 minutes) and low cost (~£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD.</abstract><type>Journal Article</type><journal>Biosensors and Bioelectronics: X</journal><volume>20</volume><journalNumber/><paginationStart>100513</paginationStart><paginationEnd/><publisher>Elsevier BV</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>2590-1370</issnPrint><issnElectronic/><keywords>Alzheimer&amp;apos; disease, Tau protein, Biomarker</keywords><publishedDay>1</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-10-01</publishedDate><doi>10.1016/j.biosx.2024.100513</doi><url/><notes/><college>COLLEGE NANME</college><department>Engineering and Applied Sciences School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>EAAS</DepartmentCode><institution>Swansea University</institution><apcterm>External research funder(s) paid the OA fee (includes OA grants disbursed by the Library)</apcterm><funders>The authors thank the MRC-AMED UK-Japan project (Multi-analyte prognostic and diagnostic screening in blood and skin for Alzheimer's disease, MR/X02153X/1) for their support. We also thank AIM providing SEM, EDS, and XPS facilities. AIM is funded in part by the EPSRC (EP/M028267/1), the European Regional Development Fund through the Welsh Government (80708) and the Welsh Government's Ser Cymru Program.</funders><projectreference/><lastEdited>2024-07-25T13:28:15.3350919</lastEdited><Created>2024-07-02T15:38:59.2727799</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Biomedical Engineering</level></path><authors><author><firstname>Arjun</firstname><surname>Ajith Mohan</surname><orcid>0000-0001-6781-2108</orcid><order>1</order></author><author><firstname>Sudhaunsh</firstname><surname>Deshpande</surname><order>2</order></author><author><firstname>Tom</firstname><surname>Dunlop</surname><orcid>0000-0002-5851-8713</orcid><order>3</order></author><author><firstname>Beth</firstname><surname>Norman</surname><order>4</order></author><author><firstname>Daniela</firstname><surname>Oliviera</surname><order>5</order></author><author><firstname>Georgeta</firstname><surname>Vulpe</surname><order>6</order></author><author><firstname>Felismina</firstname><surname>Moreira</surname><orcid>0000-0003-4237-8952</orcid><order>7</order></author><author><firstname>Sanjiv</firstname><surname>Sharma</surname><orcid>0000-0003-3828-737X</orcid><order>8</order></author></authors><documents><document><filename>66925__30982__0abfbc8f431845d1b0a758fe9ac51d14.pdf</filename><originalFilename>66925.VoR.pdf</originalFilename><uploaded>2024-07-25T13:25:23.0978146</uploaded><type>Output</type><contentLength>5699948</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2024 The Author(s). This is an open access article under the CC BY license.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs><OutputDur><Id>261</Id><DataControllerName>Sanjiv Sharma</DataControllerName><DataControllerEmail>sanjiv.sharma@swansea.ac.uk</DataControllerEmail><IsDataAvailableOnline>false</IsDataAvailableOnline><DataNotAvailableOnlineReasonId>4</DataNotAvailableOnlineReasonId><IsDurRestrictions xsi:nil="true"/><DurRestrictionReasonId xsi:nil="true"/><DurEmbargoDate xsi:nil="true"/></OutputDur></OutputDurs></rfc1807>
spelling v2 66925 2024-07-02 Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers 852f42c2a4dd3f47711475ca17030bf9 0000-0001-6781-2108 Arjun Ajith Mohan Arjun Ajith Mohan true false 809395460ab1e6b53a906b136d919c41 0000-0002-5851-8713 Tom Dunlop Tom Dunlop true false d1d5815c60d63534a10a81489255c681 Georgeta Vulpe Georgeta Vulpe true false b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2024-07-02 EAAS Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (~32 minutes) and low cost (~£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD. Journal Article Biosensors and Bioelectronics: X 20 100513 Elsevier BV 2590-1370 Alzheimer&apos; disease, Tau protein, Biomarker 1 10 2024 2024-10-01 10.1016/j.biosx.2024.100513 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) The authors thank the MRC-AMED UK-Japan project (Multi-analyte prognostic and diagnostic screening in blood and skin for Alzheimer's disease, MR/X02153X/1) for their support. We also thank AIM providing SEM, EDS, and XPS facilities. AIM is funded in part by the EPSRC (EP/M028267/1), the European Regional Development Fund through the Welsh Government (80708) and the Welsh Government's Ser Cymru Program. 2024-07-25T13:28:15.3350919 2024-07-02T15:38:59.2727799 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Arjun Ajith Mohan 0000-0001-6781-2108 1 Sudhaunsh Deshpande 2 Tom Dunlop 0000-0002-5851-8713 3 Beth Norman 4 Daniela Oliviera 5 Georgeta Vulpe 6 Felismina Moreira 0000-0003-4237-8952 7 Sanjiv Sharma 0000-0003-3828-737X 8 66925__30982__0abfbc8f431845d1b0a758fe9ac51d14.pdf 66925.VoR.pdf 2024-07-25T13:25:23.0978146 Output 5699948 application/pdf Version of Record true © 2024 The Author(s). This is an open access article under the CC BY license. true eng http://creativecommons.org/licenses/by/4.0/ 261 Sanjiv Sharma sanjiv.sharma@swansea.ac.uk false 4
title Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
spellingShingle Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
Arjun Ajith Mohan
Tom Dunlop
Georgeta Vulpe
Sanjiv Sharma
title_short Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_full Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_fullStr Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_full_unstemmed Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_sort Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
author_id_str_mv 852f42c2a4dd3f47711475ca17030bf9
809395460ab1e6b53a906b136d919c41
d1d5815c60d63534a10a81489255c681
b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv 852f42c2a4dd3f47711475ca17030bf9_***_Arjun Ajith Mohan
809395460ab1e6b53a906b136d919c41_***_Tom Dunlop
d1d5815c60d63534a10a81489255c681_***_Georgeta Vulpe
b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Arjun Ajith Mohan
Tom Dunlop
Georgeta Vulpe
Sanjiv Sharma
author2 Arjun Ajith Mohan
Sudhaunsh Deshpande
Tom Dunlop
Beth Norman
Daniela Oliviera
Georgeta Vulpe
Felismina Moreira
Sanjiv Sharma
format Journal article
container_title Biosensors and Bioelectronics: X
container_volume 20
container_start_page 100513
publishDate 2024
institution Swansea University
issn 2590-1370
doi_str_mv 10.1016/j.biosx.2024.100513
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (~32 minutes) and low cost (~£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD.
published_date 2024-10-01T13:28:14Z
_version_ 1805554018864332800
score 11.035655

Similar Items