No Cover Image

Journal article 3 views

Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers

Ajith Mohan Arjun Orcid Logo, Sudhaunsh Deshpande, Tom Dunlop Orcid Logo, Beth Norman, Daniela Oliviera, Georgeta Vulpe, Felismina Moreira, Sanjiv Sharma Orcid Logo

Biosensors and Bioelectronics:X

Swansea University Authors: Tom Dunlop Orcid Logo, Georgeta Vulpe, Sanjiv Sharma Orcid Logo

Abstract

Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI M...

Full description

Published in: Biosensors and Bioelectronics:X
Published:
URI: https://cronfa.swan.ac.uk/Record/cronfa66925
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2024-07-02T14:52:41Z
last_indexed 2024-07-02T14:52:41Z
id cronfa66925
recordtype SURis
fullrecord <?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>66925</id><entry>2024-07-02</entry><title>Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers</title><swanseaauthors><author><sid>809395460ab1e6b53a906b136d919c41</sid><ORCID>0000-0002-5851-8713</ORCID><firstname>Tom</firstname><surname>Dunlop</surname><name>Tom Dunlop</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>d1d5815c60d63534a10a81489255c681</sid><firstname>Georgeta</firstname><surname>Vulpe</surname><name>Georgeta Vulpe</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>b6b7506358522f607b171ec9c94757b7</sid><ORCID>0000-0003-3828-737X</ORCID><firstname>Sanjiv</firstname><surname>Sharma</surname><name>Sanjiv Sharma</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2024-07-02</date><abstract>Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (~32 minutes) and low cost (~£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD.</abstract><type>Journal Article</type><journal>Biosensors and Bioelectronics:X</journal><volume/><journalNumber/><paginationStart/><paginationEnd/><publisher/><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic/><keywords/><publishedDay>0</publishedDay><publishedMonth>0</publishedMonth><publishedYear>0</publishedYear><publishedDate>0001-01-01</publishedDate><doi/><url/><notes/><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><apcterm/><funders>The authors thank the MRC-AMED UK-Japan project (Multi-analyte prognostic and diagnostic screening in blood and skin for Alzheimer's disease, MR/X02153X/1) for their support. We also thank AIM providing SEM, EDS, and XPS facilities. AIM is funded in part by the EPSRC (EP/M028267/1), the European Regional Development Fund through the Welsh Government (80708) and the Welsh Government's Ser Cymru Program.</funders><projectreference/><lastEdited>2024-07-02T15:59:04.6430298</lastEdited><Created>2024-07-02T15:38:59.2727799</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Biomedical Engineering</level></path><authors><author><firstname>Ajith Mohan</firstname><surname>Arjun</surname><orcid>0000-0001-6781-2108</orcid><order>1</order></author><author><firstname>Sudhaunsh</firstname><surname>Deshpande</surname><order>2</order></author><author><firstname>Tom</firstname><surname>Dunlop</surname><orcid>0000-0002-5851-8713</orcid><order>3</order></author><author><firstname>Beth</firstname><surname>Norman</surname><order>4</order></author><author><firstname>Daniela</firstname><surname>Oliviera</surname><order>5</order></author><author><firstname>Georgeta</firstname><surname>Vulpe</surname><order>6</order></author><author><firstname>Felismina</firstname><surname>Moreira</surname><order>7</order></author><author><firstname>Sanjiv</firstname><surname>Sharma</surname><orcid>0000-0003-3828-737X</orcid><order>8</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling v2 66925 2024-07-02 Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers 809395460ab1e6b53a906b136d919c41 0000-0002-5851-8713 Tom Dunlop Tom Dunlop true false d1d5815c60d63534a10a81489255c681 Georgeta Vulpe Georgeta Vulpe true false b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2024-07-02 Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (~32 minutes) and low cost (~£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD. Journal Article Biosensors and Bioelectronics:X 0 0 0 0001-01-01 COLLEGE NANME COLLEGE CODE Swansea University The authors thank the MRC-AMED UK-Japan project (Multi-analyte prognostic and diagnostic screening in blood and skin for Alzheimer's disease, MR/X02153X/1) for their support. We also thank AIM providing SEM, EDS, and XPS facilities. AIM is funded in part by the EPSRC (EP/M028267/1), the European Regional Development Fund through the Welsh Government (80708) and the Welsh Government's Ser Cymru Program. 2024-07-02T15:59:04.6430298 2024-07-02T15:38:59.2727799 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Ajith Mohan Arjun 0000-0001-6781-2108 1 Sudhaunsh Deshpande 2 Tom Dunlop 0000-0002-5851-8713 3 Beth Norman 4 Daniela Oliviera 5 Georgeta Vulpe 6 Felismina Moreira 7 Sanjiv Sharma 0000-0003-3828-737X 8
title Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
spellingShingle Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
Tom Dunlop
Georgeta Vulpe
Sanjiv Sharma
title_short Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_full Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_fullStr Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_full_unstemmed Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
title_sort Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
author_id_str_mv 809395460ab1e6b53a906b136d919c41
d1d5815c60d63534a10a81489255c681
b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv 809395460ab1e6b53a906b136d919c41_***_Tom Dunlop
d1d5815c60d63534a10a81489255c681_***_Georgeta Vulpe
b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Tom Dunlop
Georgeta Vulpe
Sanjiv Sharma
author2 Ajith Mohan Arjun
Sudhaunsh Deshpande
Tom Dunlop
Beth Norman
Daniela Oliviera
Georgeta Vulpe
Felismina Moreira
Sanjiv Sharma
format Journal article
container_title Biosensors and Bioelectronics:X
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 0
active_str 0
description Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (~32 minutes) and low cost (~£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD.
published_date 0001-01-01T15:59:03Z
_version_ 1803479776906706944
score 11.012947

Similar Items