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PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
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Llinos Harris ,
Rowena Jenkins ,
Laurie P. Shornick
Frontiers in Microbiology, Volume: 15
Swansea University Authors:
Thomas Wilkinson , Llinos Harris , Rowena Jenkins
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© 2024 Hilliard, Wilkinson, Harris, Jenkins and Shornick. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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DOI (Published version): 10.3389/fmicb.2024.1440658
Abstract
Introduction: Bacterial infection and biofilm formation contribute to impaired healing in chronic diabetic wounds. Staphylococcus aureus and Pseudomonas aeruginosa are found in human diabetic wound biofilms. They may develop antibiotic resistance, increasing the urgency for alternative or complement...
| Published in: | Frontiers in Microbiology |
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| ISSN: | 1664-302X |
| Published: |
Frontiers Media SA
2024
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa67500 |
| Abstract: |
Introduction: Bacterial infection and biofilm formation contribute to impaired healing in chronic diabetic wounds. Staphylococcus aureus and Pseudomonas aeruginosa are found in human diabetic wound biofilms. They may develop antibiotic resistance, increasing the urgency for alternative or complementary therapies. Diabetic wound healing may be improved with the use of biomedically engineered scaffolds, which can also serve as delivery systems for antibacterial compounds. Manuka honey is a potent antibacterial and wound care agent due to its high osmolarity, low pH, and constituents (such as methylglyoxal). Honey exhibits bacteriostatic and bactericidal effects, modulates the expression of biofilm forming genes, and restores antibiotic susceptibility in previously drug resistant pathogens.Methods: In this study, we created a dermal regeneration template (DRT) composed of polycaprolactone-gelatin (PCL-gelatin) and Manuka honey to retain honey in the wound and also provide a scaffold for tissue regeneration.Results and discussion: Soluble Manuka honey inhibited the planktonic and biofilm growth of both S. aureus (UWH3) and P. aeruginosa (PA14) co-cultures. Manuka honey embedded PCL-gelatin scaffolds did not exhibit bacteriostatic or bactericidal effects on cocultures of UHW3 and PA14; however, they promoted the expression of AgrA, a gene associated with dispersal of S. aureus biofilms. |
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| Keywords: |
biofilm, honey, bacteria, wound care, ESKAPE pathogens |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
The author(s) declare that financial support was received for the
research, authorship, and/or publication of this article. GH gratefully
acknowledges financial support for this project by the Fulbright
U.S. Student Program, which is sponsored by the U.S. Department of
State and the US-UK Fulbright Commission. Additionally, Global
Wales was involved in the sponsorship of her grant “The Fulbright
Global Wales Visiting Student Researcher Award”. The contents of this
publication are solely the responsibility of the author and do not
necessarily represent the official views of the Fulbright Program, the
Government of the United States, the US-UK Fulbright Commission,
or Global Wales. |