No Cover Image

Journal article 37 views 1 download

PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa

Genevieve M. Hilliard, Thomas Wilkinson Orcid Logo, Llinos Harris Orcid Logo, Rowena Jenkins Orcid Logo, Laurie P. Shornick

Frontiers in Microbiology, Volume: 15

Swansea University Authors: Thomas Wilkinson Orcid Logo, Llinos Harris Orcid Logo, Rowena Jenkins Orcid Logo

  • 67500.VoR.pdf

    PDF | Version of Record

    © 2024 Hilliard, Wilkinson, Harris, Jenkins and Shornick. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

    Download (2.4MB)

Check full text

DOI (Published version): 10.3389/fmicb.2024.1440658

Abstract

Introduction: Bacterial infection and biofilm formation contribute to impaired healing in chronic diabetic wounds. Staphylococcus aureus and Pseudomonas aeruginosa are found in human diabetic wound biofilms. They may develop antibiotic resistance, increasing the urgency for alternative or complement...

Full description

Published in: Frontiers in Microbiology
ISSN: 1664-302X
Published: Frontiers Media SA 2024
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa67500
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2024-08-30T08:22:13Z
last_indexed 2024-08-30T08:22:13Z
id cronfa67500
recordtype SURis
fullrecord <?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>67500</id><entry>2024-08-30</entry><title>PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa</title><swanseaauthors><author><sid>86cca6bf31bfe8572de27c1b441420d8</sid><ORCID>0000-0003-0397-6079</ORCID><firstname>Thomas</firstname><surname>Wilkinson</surname><name>Thomas Wilkinson</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>dc70f9d4badbbdb5d467fd321986d173</sid><ORCID>0000-0002-0295-3038</ORCID><firstname>Llinos</firstname><surname>Harris</surname><name>Llinos Harris</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>caf58cd775a27bf8b9ee340079a29cc0</sid><ORCID>0000-0001-6664-6099</ORCID><firstname>Rowena</firstname><surname>Jenkins</surname><name>Rowena Jenkins</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2024-08-30</date><deptcode>MEDS</deptcode><abstract>Introduction: Bacterial infection and biofilm formation contribute to impaired healing in chronic diabetic wounds. Staphylococcus aureus and Pseudomonas aeruginosa are found in human diabetic wound biofilms. They may develop antibiotic resistance, increasing the urgency for alternative or complementary therapies. Diabetic wound healing may be improved with the use of biomedically engineered scaffolds, which can also serve as delivery systems for antibacterial compounds. Manuka honey is a potent antibacterial and wound care agent due to its high osmolarity, low pH, and constituents (such as methylglyoxal). Honey exhibits bacteriostatic and bactericidal effects, modulates the expression of biofilm forming genes, and restores antibiotic susceptibility in previously drug resistant pathogens.Methods: In this study, we created a dermal regeneration template (DRT) composed of polycaprolactone-gelatin (PCL-gelatin) and Manuka honey to retain honey in the wound and also provide a scaffold for tissue regeneration.Results and discussion: Soluble Manuka honey inhibited the planktonic and biofilm growth of both S. aureus (UWH3) and P. aeruginosa (PA14) co-cultures. Manuka honey embedded PCL-gelatin scaffolds did not exhibit bacteriostatic or bactericidal effects on cocultures of UHW3 and PA14; however, they promoted the expression of AgrA, a gene associated with dispersal of S. aureus biofilms.</abstract><type>Journal Article</type><journal>Frontiers in Microbiology</journal><volume>15</volume><journalNumber/><paginationStart/><paginationEnd/><publisher>Frontiers Media SA</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>1664-302X</issnElectronic><keywords>biofilm, honey, bacteria, wound care, ESKAPE pathogens</keywords><publishedDay>3</publishedDay><publishedMonth>9</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-09-03</publishedDate><doi>10.3389/fmicb.2024.1440658</doi><url/><notes/><college>COLLEGE NANME</college><department>Medical School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDS</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. GH gratefully acknowledges financial support for this project by the Fulbright U.S. Student Program, which is sponsored by the U.S. Department of State and the US-UK Fulbright Commission. Additionally, Global Wales was involved in the sponsorship of her grant “The Fulbright Global Wales Visiting Student Researcher Award”. The contents of this publication are solely the responsibility of the author and do not necessarily represent the official views of the Fulbright Program, the Government of the United States, the US-UK Fulbright Commission, or Global Wales.</funders><projectreference/><lastEdited>2024-09-19T13:21:40.5492174</lastEdited><Created>2024-08-30T09:18:35.7721675</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Biomedical Science</level></path><authors><author><firstname>Genevieve M.</firstname><surname>Hilliard</surname><order>1</order></author><author><firstname>Thomas</firstname><surname>Wilkinson</surname><orcid>0000-0003-0397-6079</orcid><order>2</order></author><author><firstname>Llinos</firstname><surname>Harris</surname><orcid>0000-0002-0295-3038</orcid><order>3</order></author><author><firstname>Rowena</firstname><surname>Jenkins</surname><orcid>0000-0001-6664-6099</orcid><order>4</order></author><author><firstname>Laurie P.</firstname><surname>Shornick</surname><order>5</order></author></authors><documents><document><filename>67500__31385__e43ba7889cca43cc943ea5757246682a.pdf</filename><originalFilename>67500.VoR.pdf</originalFilename><uploaded>2024-09-19T13:20:25.1636157</uploaded><type>Output</type><contentLength>2518456</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2024 Hilliard, Wilkinson, Harris, Jenkins and Shornick. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling v2 67500 2024-08-30 PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa 86cca6bf31bfe8572de27c1b441420d8 0000-0003-0397-6079 Thomas Wilkinson Thomas Wilkinson true false dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false caf58cd775a27bf8b9ee340079a29cc0 0000-0001-6664-6099 Rowena Jenkins Rowena Jenkins true false 2024-08-30 MEDS Introduction: Bacterial infection and biofilm formation contribute to impaired healing in chronic diabetic wounds. Staphylococcus aureus and Pseudomonas aeruginosa are found in human diabetic wound biofilms. They may develop antibiotic resistance, increasing the urgency for alternative or complementary therapies. Diabetic wound healing may be improved with the use of biomedically engineered scaffolds, which can also serve as delivery systems for antibacterial compounds. Manuka honey is a potent antibacterial and wound care agent due to its high osmolarity, low pH, and constituents (such as methylglyoxal). Honey exhibits bacteriostatic and bactericidal effects, modulates the expression of biofilm forming genes, and restores antibiotic susceptibility in previously drug resistant pathogens.Methods: In this study, we created a dermal regeneration template (DRT) composed of polycaprolactone-gelatin (PCL-gelatin) and Manuka honey to retain honey in the wound and also provide a scaffold for tissue regeneration.Results and discussion: Soluble Manuka honey inhibited the planktonic and biofilm growth of both S. aureus (UWH3) and P. aeruginosa (PA14) co-cultures. Manuka honey embedded PCL-gelatin scaffolds did not exhibit bacteriostatic or bactericidal effects on cocultures of UHW3 and PA14; however, they promoted the expression of AgrA, a gene associated with dispersal of S. aureus biofilms. Journal Article Frontiers in Microbiology 15 Frontiers Media SA 1664-302X biofilm, honey, bacteria, wound care, ESKAPE pathogens 3 9 2024 2024-09-03 10.3389/fmicb.2024.1440658 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Another institution paid the OA fee The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. GH gratefully acknowledges financial support for this project by the Fulbright U.S. Student Program, which is sponsored by the U.S. Department of State and the US-UK Fulbright Commission. Additionally, Global Wales was involved in the sponsorship of her grant “The Fulbright Global Wales Visiting Student Researcher Award”. The contents of this publication are solely the responsibility of the author and do not necessarily represent the official views of the Fulbright Program, the Government of the United States, the US-UK Fulbright Commission, or Global Wales. 2024-09-19T13:21:40.5492174 2024-08-30T09:18:35.7721675 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Genevieve M. Hilliard 1 Thomas Wilkinson 0000-0003-0397-6079 2 Llinos Harris 0000-0002-0295-3038 3 Rowena Jenkins 0000-0001-6664-6099 4 Laurie P. Shornick 5 67500__31385__e43ba7889cca43cc943ea5757246682a.pdf 67500.VoR.pdf 2024-09-19T13:20:25.1636157 Output 2518456 application/pdf Version of Record true © 2024 Hilliard, Wilkinson, Harris, Jenkins and Shornick. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng http://creativecommons.org/licenses/by/4.0/
title PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
spellingShingle PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
Thomas Wilkinson
Llinos Harris
Rowena Jenkins
title_short PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
title_full PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
title_fullStr PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
title_full_unstemmed PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
title_sort PCL-gelatin honey scaffolds promote Staphylococcus aureus agrA expression in biofilms with Pseudomonas aeruginosa
author_id_str_mv 86cca6bf31bfe8572de27c1b441420d8
dc70f9d4badbbdb5d467fd321986d173
caf58cd775a27bf8b9ee340079a29cc0
author_id_fullname_str_mv 86cca6bf31bfe8572de27c1b441420d8_***_Thomas Wilkinson
dc70f9d4badbbdb5d467fd321986d173_***_Llinos Harris
caf58cd775a27bf8b9ee340079a29cc0_***_Rowena Jenkins
author Thomas Wilkinson
Llinos Harris
Rowena Jenkins
author2 Genevieve M. Hilliard
Thomas Wilkinson
Llinos Harris
Rowena Jenkins
Laurie P. Shornick
format Journal article
container_title Frontiers in Microbiology
container_volume 15
publishDate 2024
institution Swansea University
issn 1664-302X
doi_str_mv 10.3389/fmicb.2024.1440658
publisher Frontiers Media SA
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
active_str 0
description Introduction: Bacterial infection and biofilm formation contribute to impaired healing in chronic diabetic wounds. Staphylococcus aureus and Pseudomonas aeruginosa are found in human diabetic wound biofilms. They may develop antibiotic resistance, increasing the urgency for alternative or complementary therapies. Diabetic wound healing may be improved with the use of biomedically engineered scaffolds, which can also serve as delivery systems for antibacterial compounds. Manuka honey is a potent antibacterial and wound care agent due to its high osmolarity, low pH, and constituents (such as methylglyoxal). Honey exhibits bacteriostatic and bactericidal effects, modulates the expression of biofilm forming genes, and restores antibiotic susceptibility in previously drug resistant pathogens.Methods: In this study, we created a dermal regeneration template (DRT) composed of polycaprolactone-gelatin (PCL-gelatin) and Manuka honey to retain honey in the wound and also provide a scaffold for tissue regeneration.Results and discussion: Soluble Manuka honey inhibited the planktonic and biofilm growth of both S. aureus (UWH3) and P. aeruginosa (PA14) co-cultures. Manuka honey embedded PCL-gelatin scaffolds did not exhibit bacteriostatic or bactericidal effects on cocultures of UHW3 and PA14; however, they promoted the expression of AgrA, a gene associated with dispersal of S. aureus biofilms.
published_date 2024-09-03T13:21:40Z
_version_ 1810627035280703488
score 11.028798

Similar Items