Journal article 27 views
Determining the efficacy of disinfectants at nucleic acid degradation
Journal of Applied Microbiology, Volume: 134, Issue: 11
Swansea University Authors: Kirsty Meldrum, CAITLIN MAGGS, Bethan Thomas, Natalie De Mello
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DOI (Published version): 10.1093/jambio/lxad244
Abstract
AimsNucleic acids, particularly antibiotic resistance genes, are commonly found on surfaces within healthcare environments, with levels not reducing following cleaning. Within the UK, there are no regulations for testing disinfectants against nucleic acids.Methods and resultsA series of commonplace...
Published in: | Journal of Applied Microbiology |
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ISSN: | 1365-2672 |
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Oxford University Press (OUP)
2023
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URI: | https://cronfa.swan.ac.uk/Record/cronfa67706 |
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v2 67706 2024-09-17 Determining the efficacy of disinfectants at nucleic acid degradation bbb7bd27bfa3c6ffc73da8facfebc793 Kirsty Meldrum Kirsty Meldrum true false 40ec3b849333c2ad4d354d9c76cef2d1 CAITLIN MAGGS CAITLIN MAGGS true false 09ea6b20932c7cf460b9e3459130be1f Bethan Thomas Bethan Thomas true false 0790466dcb939bd39da7d5bd6326f1b6 0000-0001-6973-2881 Natalie De Mello Natalie De Mello true false 2024-09-17 MEDS AimsNucleic acids, particularly antibiotic resistance genes, are commonly found on surfaces within healthcare environments, with levels not reducing following cleaning. Within the UK, there are no regulations for testing disinfectants against nucleic acids.Methods and resultsA series of commonplace in vitro methods were used to determine disinfectant-induced physical and functional damage to various nucleic acids; RNA (10 μg), genomic DNA (2 μg), and plasmids (1 μg). Using these methods, the optimal residence time (10 minutes) and working concentration (10%) were determined for a new disinfectant. Furthermore, comparison of disinfectants with different active ingredients including lactic acid (LA), sodium hydroxide (NaOH), chloroxylenol (PCMX), and quaternary ammonium compounds (QACs), were compared to controls. All disinfectants showed greater degradation by gel electrophoresis of genomic DNA and RNA than of purified plasmids. Functional analysis using quantitative polymerase chain reaction (qPCR) and polymerase chain reaction (PCR) demonstrated that no disinfectant tested (apart from control) could damage DNA to the level where PCR amplification was not possible, and only the NaOH reagent could achieve this for RNA.ConclusionsThe set of methods described herein provides a platform for future standardization and potential regulation regarding monitoring cleaning solutions for their activity against nucleic acids. Journal Article Journal of Applied Microbiology 134 11 Oxford University Press (OUP) 1365-2672 1 11 2023 2023-11-01 10.1093/jambio/lxad244 http://dx.doi.org/10.1093/jambio/lxad244 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2024-09-17T16:41:53.3782045 2024-09-17T16:32:17.8087309 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Rachael C Wilkinson 0000-0002-0749-108x 1 Kirsty Meldrum 2 CAITLIN MAGGS 3 Nerissa E Thomas 0009-0006-1024-8327 4 Bethan Thomas 5 Natalie De Mello 0000-0001-6973-2881 6 Naomi Joyce 0000-0002-0380-6662 7 |
title |
Determining the efficacy of disinfectants at nucleic acid degradation |
spellingShingle |
Determining the efficacy of disinfectants at nucleic acid degradation Kirsty Meldrum CAITLIN MAGGS Bethan Thomas Natalie De Mello |
title_short |
Determining the efficacy of disinfectants at nucleic acid degradation |
title_full |
Determining the efficacy of disinfectants at nucleic acid degradation |
title_fullStr |
Determining the efficacy of disinfectants at nucleic acid degradation |
title_full_unstemmed |
Determining the efficacy of disinfectants at nucleic acid degradation |
title_sort |
Determining the efficacy of disinfectants at nucleic acid degradation |
author_id_str_mv |
bbb7bd27bfa3c6ffc73da8facfebc793 40ec3b849333c2ad4d354d9c76cef2d1 09ea6b20932c7cf460b9e3459130be1f 0790466dcb939bd39da7d5bd6326f1b6 |
author_id_fullname_str_mv |
bbb7bd27bfa3c6ffc73da8facfebc793_***_Kirsty Meldrum 40ec3b849333c2ad4d354d9c76cef2d1_***_CAITLIN MAGGS 09ea6b20932c7cf460b9e3459130be1f_***_Bethan Thomas 0790466dcb939bd39da7d5bd6326f1b6_***_Natalie De Mello |
author |
Kirsty Meldrum CAITLIN MAGGS Bethan Thomas Natalie De Mello |
author2 |
Rachael C Wilkinson Kirsty Meldrum CAITLIN MAGGS Nerissa E Thomas Bethan Thomas Natalie De Mello Naomi Joyce |
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Journal article |
container_title |
Journal of Applied Microbiology |
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134 |
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11 |
publishDate |
2023 |
institution |
Swansea University |
issn |
1365-2672 |
doi_str_mv |
10.1093/jambio/lxad244 |
publisher |
Oxford University Press (OUP) |
college_str |
Faculty of Medicine, Health and Life Sciences |
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|
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science |
url |
http://dx.doi.org/10.1093/jambio/lxad244 |
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description |
AimsNucleic acids, particularly antibiotic resistance genes, are commonly found on surfaces within healthcare environments, with levels not reducing following cleaning. Within the UK, there are no regulations for testing disinfectants against nucleic acids.Methods and resultsA series of commonplace in vitro methods were used to determine disinfectant-induced physical and functional damage to various nucleic acids; RNA (10 μg), genomic DNA (2 μg), and plasmids (1 μg). Using these methods, the optimal residence time (10 minutes) and working concentration (10%) were determined for a new disinfectant. Furthermore, comparison of disinfectants with different active ingredients including lactic acid (LA), sodium hydroxide (NaOH), chloroxylenol (PCMX), and quaternary ammonium compounds (QACs), were compared to controls. All disinfectants showed greater degradation by gel electrophoresis of genomic DNA and RNA than of purified plasmids. Functional analysis using quantitative polymerase chain reaction (qPCR) and polymerase chain reaction (PCR) demonstrated that no disinfectant tested (apart from control) could damage DNA to the level where PCR amplification was not possible, and only the NaOH reagent could achieve this for RNA.ConclusionsThe set of methods described herein provides a platform for future standardization and potential regulation regarding monitoring cleaning solutions for their activity against nucleic acids. |
published_date |
2023-11-01T16:41:51Z |
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1810458436502028288 |
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11.028798 |