Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus

Seeley, Aidan; MAHMOOD, ROMESSA; BELLAMY, CAITLIN; ROOME, ELIS; WILLIAMS, BENJAMIN; Davies, Nia; Wallace, Lisa

doi:10.1111/gbb.70006

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Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus

Aidan Seeley

, ROMESSA MAHMOOD, CAITLIN BELLAMY, ELIS ROOME, BENJAMIN WILLIAMS, Nia Davies, Lisa Wallace

Genes, Brain and Behavior, Volume: 23, Issue: 5, Start page: e70006

Swansea University Authors: Aidan Seeley , ROMESSA MAHMOOD, CAITLIN BELLAMY, ELIS ROOME, BENJAMIN WILLIAMS, Nia Davies, Lisa Wallace

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DOI (Published version): 10.1111/gbb.70006

Abstract

Ethanol is one of the most widely used drugs in the world. Ethanol induces profound physiological and behavioural responses in invertebrate model organisms, such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus (Annelida, Oligochaete) is an aquatic worm which shows behav...

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Published in:	Genes, Brain and Behavior
ISSN:	1601-1848 1601-183X
Published:	Wiley 2024
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa68104

first_indexed	2024-10-29T15:41:51Z
last_indexed	2024-11-25T14:21:26Z
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Ethanol induces profound physiological and behavioural responses in invertebrate model organisms, such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus (Annelida, Oligochaete) is an aquatic worm which shows behavioural responses to common drugs and thus is potentially useful in pharmacological research. The effects of ethanol are unknown in this organism. In this study, we examine the effects of acute exposure to ethanol (0-500 mM) on the stereotypical movements and locomotor activity of L. variegatus and examine the concentration- (0-500 mM) and time-dependent (0-210 min) effects of ethanol in L. variegatus. We show that ≥250 mM ethanol reversibly reduced the ability of tactile stimulation to elicit stereotypical movements, namely body reversal and helical swimming and locomotor activity (p < 0.05, N = 8). We also found that 2 min of exposure to ≥250 mM ethanol rapidly induces steady-state hypokinesis (p < 0.05, N = 11) and confirm ethanol absorption into L. variegatus tissues. Additionally, we also observed acute ethanol tolerance after 150 min of exposure to 500 mM ethanol (p < 0.05, N = 24). This study is the first to report the behavioural effects of ethanol in L. variegatus. Our results show that this is a model organism for use in ethanol studies, providing further evidence for its utility in pharmacological research. [Abstract copyright: © 2024 The Author(s). 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spelling	2024-10-29T15:52:02.0799202 v2 68104 2024-10-29 Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus c69dba86b3ccf9a140b67b7e97d68bba 0000-0001-7085-4296 Aidan Seeley Aidan Seeley true false ef1f983edbeb76c80ca1b3374f89b8b3 ROMESSA MAHMOOD ROMESSA MAHMOOD true false e9321656ab2f6f9dc30f5e1bb3605ba3 CAITLIN BELLAMY CAITLIN BELLAMY true false 697dcdb246cfc62876530b805cfb8b8b ELIS ROOME ELIS ROOME true true 048456a98338bc8229dd2913bc3fdd06 BENJAMIN WILLIAMS BENJAMIN WILLIAMS true true 977abe5c673627024e4913d034dcbc95 Nia Davies Nia Davies true false 5cebf16bdbc8022118a35da9d13f5087 0000-0001-7471-9833 Lisa Wallace Lisa Wallace true false 2024-10-29 MEDS Ethanol is one of the most widely used drugs in the world. Ethanol induces profound physiological and behavioural responses in invertebrate model organisms, such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus (Annelida, Oligochaete) is an aquatic worm which shows behavioural responses to common drugs and thus is potentially useful in pharmacological research. The effects of ethanol are unknown in this organism. In this study, we examine the effects of acute exposure to ethanol (0-500 mM) on the stereotypical movements and locomotor activity of L. variegatus and examine the concentration- (0-500 mM) and time-dependent (0-210 min) effects of ethanol in L. variegatus. We show that ≥250 mM ethanol reversibly reduced the ability of tactile stimulation to elicit stereotypical movements, namely body reversal and helical swimming and locomotor activity (p < 0.05, N = 8). We also found that 2 min of exposure to ≥250 mM ethanol rapidly induces steady-state hypokinesis (p < 0.05, N = 11) and confirm ethanol absorption into L. variegatus tissues. Additionally, we also observed acute ethanol tolerance after 150 min of exposure to 500 mM ethanol (p < 0.05, N = 24). This study is the first to report the behavioural effects of ethanol in L. variegatus. Our results show that this is a model organism for use in ethanol studies, providing further evidence for its utility in pharmacological research. [Abstract copyright: © 2024 The Author(s). Genes, Brain and Behavior published by International Behavioural and Neural Genetics Society and John Wiley & Sons Ltd.] Journal Article Genes, Brain and Behavior 23 5 e70006 Wiley 1601-1848 1601-183X Behaviour, ethanol, invertebrate, Lumbriculus variegatus, tolerance 15 10 2024 2024-10-15 10.1111/gbb.70006 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University SU Library paid the OA fee (TA Institutional Deal) This work is supported by a St David's Medical Foundation Seed-Corn Grant. 2024-10-29T15:52:02.0799202 2024-10-29T15:19:02.5373496 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Aidan Seeley 0000-0001-7085-4296 1 ROMESSA MAHMOOD 2 CAITLIN BELLAMY 3 ELIS ROOME 4 BENJAMIN WILLIAMS 5 Nia Davies 6 Lisa Wallace 0000-0001-7471-9833 7 68104__32785__6dc079bea5254b438cfa0159360aa72c.pdf 68104.VOR.pdf 2024-10-29T15:39:12.2910415 Output 3935095 application/pdf Version of Record true © 2024 The Author(s). This is an open access article under the terms of the Creative Commons Attribution License (CC BY 4.0). true eng http://creativecommons.org/licenses/by/4.0/
title	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus
spellingShingle	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus Aidan Seeley ROMESSA MAHMOOD CAITLIN BELLAMY ELIS ROOME BENJAMIN WILLIAMS Nia Davies Lisa Wallace
title_short	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus
title_full	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus
title_fullStr	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus
title_full_unstemmed	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus
title_sort	Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus
author_id_str_mv	c69dba86b3ccf9a140b67b7e97d68bba ef1f983edbeb76c80ca1b3374f89b8b3 e9321656ab2f6f9dc30f5e1bb3605ba3 697dcdb246cfc62876530b805cfb8b8b 048456a98338bc8229dd2913bc3fdd06 977abe5c673627024e4913d034dcbc95 5cebf16bdbc8022118a35da9d13f5087
author_id_fullname_str_mv	c69dba86b3ccf9a140b67b7e97d68bba_*_Aidan Seeley ef1f983edbeb76c80ca1b3374f89b8b3__ROMESSA MAHMOOD e9321656ab2f6f9dc30f5e1bb3605ba3__CAITLIN BELLAMY 697dcdb246cfc62876530b805cfb8b8b__ELIS ROOME 048456a98338bc8229dd2913bc3fdd06__BENJAMIN WILLIAMS 977abe5c673627024e4913d034dcbc95__Nia Davies 5cebf16bdbc8022118a35da9d13f5087_**_Lisa Wallace
author	Aidan Seeley ROMESSA MAHMOOD CAITLIN BELLAMY ELIS ROOME BENJAMIN WILLIAMS Nia Davies Lisa Wallace
author2	Aidan Seeley ROMESSA MAHMOOD CAITLIN BELLAMY ELIS ROOME BENJAMIN WILLIAMS Nia Davies Lisa Wallace
format	Journal article
container_title	Genes, Brain and Behavior
container_volume	23
container_issue	5
container_start_page	e70006
publishDate	2024
institution	Swansea University
issn	1601-1848 1601-183X
doi_str_mv	10.1111/gbb.70006
publisher	Wiley
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
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hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description	Ethanol is one of the most widely used drugs in the world. Ethanol induces profound physiological and behavioural responses in invertebrate model organisms, such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus (Annelida, Oligochaete) is an aquatic worm which shows behavioural responses to common drugs and thus is potentially useful in pharmacological research. The effects of ethanol are unknown in this organism. In this study, we examine the effects of acute exposure to ethanol (0-500 mM) on the stereotypical movements and locomotor activity of L. variegatus and examine the concentration- (0-500 mM) and time-dependent (0-210 min) effects of ethanol in L. variegatus. We show that ≥250 mM ethanol reversibly reduced the ability of tactile stimulation to elicit stereotypical movements, namely body reversal and helical swimming and locomotor activity (p < 0.05, N = 8). We also found that 2 min of exposure to ≥250 mM ethanol rapidly induces steady-state hypokinesis (p < 0.05, N = 11) and confirm ethanol absorption into L. variegatus tissues. Additionally, we also observed acute ethanol tolerance after 150 min of exposure to 500 mM ethanol (p < 0.05, N = 24). This study is the first to report the behavioural effects of ethanol in L. variegatus. Our results show that this is a model organism for use in ethanol studies, providing further evidence for its utility in pharmacological research. [Abstract copyright: © 2024 The Author(s). Genes, Brain and Behavior published by International Behavioural and Neural Genetics Society and John Wiley & Sons Ltd.]
published_date	2024-10-15T05:48:01Z
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Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus

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