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Sharing of cmeRABC alleles between C. coli and C. jejuni associated with extensive drug resistance in Campylobacter isolates from infants and poultry in the Peruvian Amazon

Kerry K Cooper Orcid Logo, Evangelos Mourkas Orcid Logo, Francesca Schiaffino Orcid Logo, Craig T Parker Orcid Logo, Tackeshy N Pinedo Vasquez, Paul F Garcia Bardales, Pablo Peñataro Yori, Maribel Paredes Olortegui, Katia Manzanares Villanueva, Lucero Romaina Cachique, Hermann Silva Delgado, Matthew Hitchings Orcid Logo, Steven Huynh, Samuel K Sheppard Orcid Logo, Ben Pascoe Orcid Logo, Margaret N Kosek Orcid Logo

mBio, Start page: e02054-24

Swansea University Author: Matthew Hitchings Orcid Logo

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DOI (Published version): 10.1128/mbio.02054-24

Abstract

Campylobacter is a serious health threat because of the rapid progressive evolution of antimicrobial resistance and efficient transmission from zoonotic as well as human sources. Resistance to fluoroquinolones and macrolides is particularly concerning as this compromises the two most effective oral...

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Published in: mBio
ISSN: 2150-7511
Published: American Society for Microbiology (ASM) 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa68684
Abstract: Campylobacter is a serious health threat because of the rapid progressive evolution of antimicrobial resistance and efficient transmission from zoonotic as well as human sources. Resistance to fluoroquinolones and macrolides is particularly concerning as this compromises the two most effective oral antibiotic agents currently available for human campylobacteriosis. Here, we report on the prevalence and worldwide distribution of the operon cmeRABC, which encodes an efflux pump conferring high levels of combined resistance to fluoroquinolones and macrolides in Campylobacter strains isolated from poultry (n = 75) and children (n = 177). These mutations were found to be highly prevalent in isolates from poultry (62.7%) and children (29.4%) in Iquitos, Peru. We investigated the population structure of genes in the cmeRABC operon and identified a potential genetic bottleneck for the cmeA and cmeB genes. While most cmeB alleles segregate by species, alleles associated with high resistance to fluoroquinolones and macrolides were found in both Campylobacter jejuni and Campylobacter coli. We inferred that the likely ancestry of these alleles was from C. jejuni and was later acquired by C. coli through recombination. Publicly accessible global genomic data from 16,120 Campylobacter genomes identified these mutations in approximately 6% of C. jejuni and C. coli isolates globally, with higher prevalence in samples from poultry in many countries, including Peru. Our findings suggest that these extensively drug-resistant Campylobacter strains originated from C. jejuni in poultry.
Keywords: Campylobacter, efflux pump, antibiotic resistance, recombination, Iquitos
College: Faculty of Medicine, Health and Life Sciences
Funders: This publication made use of the PubMLST website (http://pubmlst.org/) developed by Keith Jolley and Martin Maiden and hosted at the University of Oxford. Funding for this study was provided by the Bill and Melinda Gates Foundation (OPP1066146 and OPP1152146 to M.N.K.) and the National Institutes of Health of the United States (R01AI158576 and R21AI163801 to M.N.K. and C.T.P.; D43TW010913 to M.N.K.; K43TW012298 to F.S.). This research was also supported in part by United States Department of Agriculture–Agricultural Research Service Current Research Information System, project 2030–42000-055-00D (to C.T.P.).
Start Page: e02054-24

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