Conference Paper/Proceeding/Abstract 681 views
Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
European Congress of Clinical Microbiology and Infectious Diseases 2016
Swansea University Author: Matthew Hitchings
Background: Accute Campylobacter gastroenteritis in developed countries is characterized by sporadic infection, peaking during infancy and early adulthood, with most infections resulting from consumption of contaminated food. In developing countries, the epidemiology of disease is quite different wi...
|Published in:||European Congress of Clinical Microbiology and Infectious Diseases 2016|
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Background: Accute Campylobacter gastroenteritis in developed countries is characterized by sporadic infection, peaking during infancy and early adulthood, with most infections resulting from consumption of contaminated food. In developing countries, the epidemiology of disease is quite different with asymptomatic unreported infection endemic in children younger than 2 years old. Asymptomatic infection is associated with poor cognitive and physical development.Material/methods: We correlate virulence and host cell response in in vitro assays with a detailed record of patient symptoms for in 101 C. jejuni isolates from a cohort of 442 children aged 0–72 months. Isolates were sequenced and genome-wide association studies were used to identify genetic elements associated with asymptomatic carriage and specific host immune responses.Results: Genome-wide association studies identified a strong association between asymptomatic carriage and putative glycosylation, motility, capsule production and iron homeostasis genes. Laboratory work is ongoing to test the specific role of these genes in phenotypes relevant to host infection, including cytokine response, invasion and growth under iron depleted conditions.Conclusions: Improvements in the understanding of disease and carriage of C. jejnui has implications for current campylobacteriosis treatment regimens, vaccine design and the development of rapid diagnostics for early detection at the point of care.
Swansea University Medical School