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Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids / Julanta Carriere

Swansea University Author: Julanta Carriere

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Abstract

The endocannabinoid system (ECS) plays a crucial role in maintaining biological balance, encompassing G protein-coupled receptors, endogenous ligands, and catabolic enzymes. This system is targeted by endogenous cannabinoids such as anandamide (AEA), 2-arachidonoyglycerol (2-AG), and Δ9-tetrahydroca...

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Published: Swansea, Wales, UK 2025
Institution: Swansea University
Degree level: Master of Research
Degree name: MSc by Research
Supervisor: Wallace, Lisa ; Seeley, Aidan ; Davies, Nia
URI: https://cronfa.swan.ac.uk/Record/cronfa68917
first_indexed 2025-02-18T12:34:29Z
last_indexed 2025-02-19T07:29:12Z
id cronfa68917
recordtype RisThesis
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spelling 2025-02-18T12:40:52.4830247 v2 68917 2025-02-18 Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids 4d91151193de7925ea048bf39f32198b Julanta Carriere Julanta Carriere true false 2025-02-18 The endocannabinoid system (ECS) plays a crucial role in maintaining biological balance, encompassing G protein-coupled receptors, endogenous ligands, and catabolic enzymes. This system is targeted by endogenous cannabinoids such as anandamide (AEA), 2-arachidonoyglycerol (2-AG), and Δ9-tetrahydrocannabinol (THC), the psychoactive constituent of the cannabis plant. Cannabidiol (CBD), which isn’t a component in the ECS, is a compound found in cannabis and has a proposed 56 molecular targets. CBD is non-psychoactive and complex in its mechanisms and only interacts with ECS receptors in the presence of THC. Utilising the freshwater invertebrate Lumbriculus variegatus as a novel in vivo model, we investigate endogenous cannabinoids, along with AEA combined with the fatty acid amide hydrolase (FAAH) inhibitor URB 597, and CBD. Our objectives were to analyse cannabinoid-like receptor proteins in vertebrates and invertebrates, determining the lethal dose for 50% of the population, assessing behavioural effects post-CBD and endocannabinoid exposure, and developing novel methodologies for drug absorption analysis. The conservation of endocannabinoid proteins in other animals suggest they have an essential role across different species. Our study reveals stereotypical movement is significantly reduced with exposure to ≥10 µM 2-AG, ≥10 µM AEA, and ≥10 µM AEA concentrations ± URB 597 (250 µM). Notably, combining AEA and URB 597 results in further movement reduction post-rescue period compared to AEA alone (≥5 µM vs. ≥10 µM). This study also shows CBD is toxic to L. variegatus at concentrations of ≥30 µM. Through GC-MS, we found CBD was undetectable at concentrations below 10 µM, which implies there is a minimum concentration threshold for CBD to be detected through GC-MS. Additionally saw CBD significantly alters stereotypical movement in L. variegatus at concentrations of ≥5 µM. We show L. variegatus could potentially serve as an invaluable model for understanding cannabinoid interactions, facilitating drug design, and highlighting environmental impacts from cannabinoid exposure. E-Thesis Swansea, Wales, UK Cannabidiol, endocannabinoid system, Lumbriculus variegatus, pharmacology, ecotoxicology 14 1 2025 2025-01-14 ORCiD identifier: https://orcid.org/0009-0006-7446-6956 COLLEGE NANME COLLEGE CODE Swansea University Wallace, Lisa ; Seeley, Aidan ; Davies, Nia Master of Research MSc by Research 2025-02-18T12:40:52.4830247 2025-02-18T12:31:10.6489613 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Julanta Carriere 1 68917__33625__fe123f29152d410fac9771790888e294.pdf Carriere_Julanta_MSc_Research_Thesis_Final_Cronfa.pdf 2025-02-18T12:40:31.1103782 Output 2723418 application/pdf E-Thesis – open access true Copyright: The Author, Julanta Carriere, 2025. true eng
title Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
spellingShingle Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
Julanta Carriere
title_short Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
title_full Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
title_fullStr Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
title_full_unstemmed Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
title_sort Investigating the response of Lumbriculus variegatus to cannabidiol and endocannabinoids
author_id_str_mv 4d91151193de7925ea048bf39f32198b
author_id_fullname_str_mv 4d91151193de7925ea048bf39f32198b_***_Julanta Carriere
author Julanta Carriere
author2 Julanta Carriere
format E-Thesis
publishDate 2025
institution Swansea University
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
active_str 0
description The endocannabinoid system (ECS) plays a crucial role in maintaining biological balance, encompassing G protein-coupled receptors, endogenous ligands, and catabolic enzymes. This system is targeted by endogenous cannabinoids such as anandamide (AEA), 2-arachidonoyglycerol (2-AG), and Δ9-tetrahydrocannabinol (THC), the psychoactive constituent of the cannabis plant. Cannabidiol (CBD), which isn’t a component in the ECS, is a compound found in cannabis and has a proposed 56 molecular targets. CBD is non-psychoactive and complex in its mechanisms and only interacts with ECS receptors in the presence of THC. Utilising the freshwater invertebrate Lumbriculus variegatus as a novel in vivo model, we investigate endogenous cannabinoids, along with AEA combined with the fatty acid amide hydrolase (FAAH) inhibitor URB 597, and CBD. Our objectives were to analyse cannabinoid-like receptor proteins in vertebrates and invertebrates, determining the lethal dose for 50% of the population, assessing behavioural effects post-CBD and endocannabinoid exposure, and developing novel methodologies for drug absorption analysis. The conservation of endocannabinoid proteins in other animals suggest they have an essential role across different species. Our study reveals stereotypical movement is significantly reduced with exposure to ≥10 µM 2-AG, ≥10 µM AEA, and ≥10 µM AEA concentrations ± URB 597 (250 µM). Notably, combining AEA and URB 597 results in further movement reduction post-rescue period compared to AEA alone (≥5 µM vs. ≥10 µM). This study also shows CBD is toxic to L. variegatus at concentrations of ≥30 µM. Through GC-MS, we found CBD was undetectable at concentrations below 10 µM, which implies there is a minimum concentration threshold for CBD to be detected through GC-MS. Additionally saw CBD significantly alters stereotypical movement in L. variegatus at concentrations of ≥5 µM. We show L. variegatus could potentially serve as an invaluable model for understanding cannabinoid interactions, facilitating drug design, and highlighting environmental impacts from cannabinoid exposure.
published_date 2025-01-14T05:25:34Z
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score 11.089572

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