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Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Mustapha Touray Orcid Logo, Harun Cimen, Edna Bode, Helge B. Bode, Selcuk Hazir

Journal of Pest Science, Volume: 97, Issue: 4, Pages: 2203 - 2215

Swansea University Author: Mustapha Touray Orcid Logo

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DOI (Published version): 10.1007/s10340-024-01760-7

Abstract

Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial s...

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Published in: Journal of Pest Science
ISSN: 1612-4758 1612-4766
Published: Springer Science and Business Media LLC 2024
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa69458
Abstract: Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products with various activities including larvicidal activity. In this study, the effects of natural products produced by four Xenorhabdus and one Photorhabdus bacteria on the ovipositional behavior of Ae. albopictus mosquitoes were assessed. Utilizing a binary choice assay in insect cages, gravid female mosquitoes were presented with two oviposition cups containing water supplemented with varying concentrations of bacterial supernatants (50–1% concentrations) versus control medium. After 72 h, the eggs deposited on filter papers were counted. The oviposition attractant index (OAI) feature of the bacterial supernatant was evaluated using the number of eggs laid in the cups. Notably, all tested supernatants exhibited concentration-dependent deterrence of oviposition. Xenorhabdus cabanillasii displayed the strongest deterrent effect, inhibiting egg-laying at 50–5% concentrations (OAI: − 0.87 to − 0.35), followed by X. nematophila (50–10%, OAI: − 0.82 to − 0.52). Xenorhabdus szentirmaii, X. doucetiae, and P. kayaii showed significant deterrence at ≥ 20% concentrations. Using promoter exchange mutants generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. Such deterrents targeting egg-laying could be valuable for controlling populations by disrupting their breeding in suitable habitats.
Keywords: Oviposition; Deterrence; Mosquito; Xenorhabdus; Photorhabdus; Fabclavines; easyPACId method
College: Faculty of Science and Engineering
Funders: Open access funding provided by the Scientific and Technological Research Council of Türkiye (TÜBİTAK). Work in Hazir lab was funded by Aydin Adnan Menderes University (project no. FEF-22014) and TUBITAK-116Z074. Work in Bode lab was supported by LOEWE TBG funded by the state of Hesse and an ERC Advanced Grant (835108).
Issue: 4
Start Page: 2203
End Page: 2215

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