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Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Sebnem Hazal Gulsen, Evren Tileklioglu, Edna Bode, Harun Cimen, Hatice Ertabaklar, Derya Ulug, Sema Ertug, Sebastian L. Wenski, Mustapha Touray Orcid Logo, Canan Hazir, Duygu Kaya Bilecenoglu, Ibrahim Yildiz, Helge B. Bode, Selcuk Hazir

Scientific Reports, Volume: 12, Issue: 1

Swansea University Author: Mustapha Touray Orcid Logo

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DOI (Published version): 10.1038/s41598-022-13722-z

Abstract

Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the a...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: Springer Science and Business Media LLC 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa69477
Abstract: Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future.
College: Faculty of Science and Engineering
Funders: Open Access funding enabled and organized by Projekt DEAL. This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK-Project Number: 116S387) and Aydin Adnan Menderes University, Project Number: 20001). Work in the Bode lab was supported by the BMBF (Project Number: 01DL17009) and the LOEWE Center TBG funded by the state of Hesse.
Issue: 1

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