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Differences in innate immune function between allergic and nonallergic children: new insights into immune ontogeny. / MK Tulic; M Hodder; al A Forsberg et; S McCarthy; T Richman; N D'Vaz; AH van den Biggelaar; CA Thornton; SL Prescott; Catherine Thornton
Journal of Allergy and Clinical Immunology, Volume: 127, Issue: 2, Pages: 470 - 478
Swansea University Author: Catherine, Thornton
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BACKGROUND: Microbial products are of central interest in the modulation of allergic propensity.OBJECTIVE: We sought to explore whether allergic children show differences in microbial Toll-like receptor (TLR)-mediated responses over their first 5 years of life.METHODS: Mononuclear cells isolated fro...
|Published in:||Journal of Allergy and Clinical Immunology|
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BACKGROUND: Microbial products are of central interest in the modulation of allergic propensity.OBJECTIVE: We sought to explore whether allergic children show differences in microbial Toll-like receptor (TLR)-mediated responses over their first 5 years of life.METHODS: Mononuclear cells isolated from 35 allergic and 35 nonallergic children at birth and 1, 2.5, and 5 years of age were stimulated with TLR2-TLR9 ligands to study innate immune function and with allergens or mitogen to assess adaptive T-cell responses. Cytokine production was measured by using Luminex multiplexing technology.RESULTS: Nonallergic children show progressive and significant age-related increases in innate cytokine responses (IL-1β, IL-6, TNF-α, and IL-10) to virtually all TLR ligands. This innate maturation corresponds with a parallel increase in adaptive T(H)1 (IFN-γ) responses to allergens and mitogens. In contrast, allergic children show exaggerated innate responses at birth (P < .01) but a relative decrease with age thereafter, so that by age 5 years, TLR responses are attenuated compared with those seen in nonallergic subjects (P < .05). This early hyperresponsiveness in allergic subjects fails to translate to a corresponding maturation of T(H)1 function, which remains attenuated relative to that seen in nonallergic subjects but is associated with a characteristic age-dependent increase in allergen-specific T(H)2 responses (P < .01).CONCLUSION: Our findings suggest significant differences in the developmental trajectory of innate immune function in children with allergic disease that might contribute to the recognized differences in postnatal adaptive T-cell immunity
allergy; immunity; early life; toll like receptors
Swansea University Medical School