No Cover Image

Journal article 594 views

Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-) regulatory T cell function. / M Smith; MR Tourigny; P Noakes; CA Thornton; MK Tulic; SL Prescott; Catherine Thornton

Journal of Allergy and Clinical Immunology, Volume: 121, Issue: 6, Pages: 1460 - 1466

Swansea University Author: Catherine, Thornton

Full text not available from this repository: check for access using links below.

Abstract

BACKGROUND: The role of regulatory T (Treg) cells in allergic predisposition is not known.OBJECTIVE: This study compared the frequency and function of cord blood Treg cells from nonallergic children (n = 18) with those from children who have egg allergy (n = 15) in the first year of life.METHODS: CD...

Full description

Published in: Journal of Allergy and Clinical Immunology
ISSN: 00916749
Published: Elsevier 2008
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa9993
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: BACKGROUND: The role of regulatory T (Treg) cells in allergic predisposition is not known.OBJECTIVE: This study compared the frequency and function of cord blood Treg cells from nonallergic children (n = 18) with those from children who have egg allergy (n = 15) in the first year of life.METHODS: CD4(+) effector T cells and autologous antigen-presenting cells isolated from cord blood mononuclear cells were cocultured with or without CD4(+)CD25(+)CD127(lo/-) Treg cells, and cytokine responses to staphylococcal endotoxin B were assessed after 48 hours.RESULTS: The addition of Treg cell populations to cord blood mononuclear cell cultures resulted in significant reduction in IL-10 (P = .002), IL-13 (P = .012), and IFN-gamma (P < .001) production. Consistent with other reports, effector CD4(+) T-cell responses (IFN-gamma and IL-13) tended to be lower in the allergic group. These neonates showed less significant Treg cell-associated suppression of IFN-gamma (P = .015) compared with that seen in the nonallergic group (P = .001). The allergic group was also less likely (44%) to show Treg cell-associated suppression of IFN-gamma effector responses compared with that seen in the nonallergic group (78%, P = .015). The magnitude of suppression (change in IFN-gamma level when CD4(+)CD25(+)CD127(lo/-) Treg cells were added to responding effector T-cell cultures) was significantly lower in the allergic group (P = .004). There were no between-group differences in the circulating CD4(+)CD25(+)CD127(lo/-) Treg cells (as a percentage of cord blood T cells) or in the FOXP3 expression of these cells.CONCLUSION: This study confirms the presence and activity of Treg cells in cord blood and provides preliminary evidence of differences in neonates who progress to allergic disease in the first year of life.
Keywords: Regulatory T cells, cord blood, neonates, food allergy, allergic disease, egg allergy, cytokines, infants
College: Swansea University Medical School
Issue: 6
Start Page: 1460
End Page: 1466