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Shotgun cholanomics of ileal fluid / Yuqin, Wang; William, Griffiths

Biochimie

Swansesa University Authors: Yuqin, Wang, William, Griffiths

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Abstract

In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn’s disease. In a pilot study we demonstrate the method by analys...

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Published in: Biochimie
ISSN: 0300-9084
Published: 2012
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa12882
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Abstract: In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn’s disease. In a pilot study we demonstrate the method by analysing the bile acid content of ileal fluid from seven Crohn’s disease patients and three healthy controls. The dominant bile acids observed were di and/or trihydroxycholanoates, di- and/or trihydroxycholanoylglycines, di- and/or trihydroxycholanoyltaurines, monosulphated dihydroxycholanoates and monosulphated dihydroxycholanoylglycine. The method can be similarly applied to samples derived from other parts of the intestine.
Keywords: Bile acid, Lipidomics, Mass spectrometry, Electrospray ionization, Crohn’s disease.
College: Swansea University Medical School