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Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1

John J. Bromfield, I. Martin Sheldon, Martin Sheldon Orcid Logo

Biology of Reproduction, Volume: 88, Issue: 4

Swansea University Author: Martin Sheldon Orcid Logo

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DOI (Published version): 10.1095/biolreprod.112.106914

Abstract

Infections of the uterus or mammary gland with Gram-negative bacteria cause infertility in cattle, not only during disease but also for some time afterward. Even though these infections are in organs distant from the ovary, metritis and mastitis perturb antral follicle development and function in vi...

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Published in: Biology of Reproduction
ISSN: 0006-3363 1529-7268
Published: 2013
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URI: https://cronfa.swan.ac.uk/Record/cronfa15413
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first_indexed 2013-08-22T01:57:48Z
last_indexed 2018-05-04T18:42:07Z
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2018-05-04T16:42:46.9414275</datestamp><bib-version>v2</bib-version><id>15413</id><entry>2013-08-09</entry><title>Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1</title><swanseaauthors><author><sid>ab0f74b794e59cc270c69e63ee1d9748</sid><ORCID>0000-0001-7902-5558</ORCID><firstname>Martin</firstname><surname>Sheldon</surname><name>Martin Sheldon</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2013-08-09</date><deptcode>BMS</deptcode><abstract>Infections of the uterus or mammary gland with Gram-negative bacteria cause infertility in cattle, not only during disease but also for some time afterward. Even though these infections are in organs distant from the ovary, metritis and mastitis perturb antral follicle development and function in vivo. Although granulosa cells from antral follicles express toll-like receptor 4 (TLR4), and detect and mount an inflammatory response to lipopolysaccharide (LPS) from Gram-negative bacteria, it is not known whether LPS impacts preantral follicle development. The present study tested the hypothesis that LPS perturbs the development of primordial ovarian follicles. Exposure of bovine ovarian cortex ex vivo to LPS reduced the primordial follicle pool associated with increased primordial follicle activation. Ovarian cortex culture supernatants accumulated the inflammatory mediators IL-1beta, IL-6, and IL-8 in an LPS concentration-dependent manner. In addition, LPS exposure modulated key intracellular regulators of follicle activation with loss of the primordial follicle PTEN and cytoplasmic translocation of FOXO3. Acute exposure of mice in vivo to LPS also reduced the primordial follicle pool associated with increased follicle atresia. The increased follicle atresia was TLR4-dependent as Tlr4-deficient mice were insensitive to LPS-mediated follicle atresia. However, LPS did not affect the diameter of individually cultured bovine secondary follicles or their enclosed oocytes. In conclusion, LPS reduced the primordial ovarian follicle pool in the bovine ovarian cortex ex vivo and in the murine ovary in vivo. These observations provide an insight into how bacterial infections distant from the ovary have long term effects on fertility.</abstract><type>Journal Article</type><journal>Biology of Reproduction</journal><volume>88</volume><journalNumber>4</journalNumber><publisher/><issnPrint>0006-3363</issnPrint><issnElectronic>1529-7268</issnElectronic><keywords>Ovary Infection Immunity</keywords><publishedDay>20</publishedDay><publishedMonth>3</publishedMonth><publishedYear>2013</publishedYear><publishedDate>2013-03-20</publishedDate><doi>10.1095/biolreprod.112.106914</doi><url>http://www.biolreprod.org/content/88/4/98.long</url><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2018-05-04T16:42:46.9414275</lastEdited><Created>2013-08-09T08:41:55.0723263</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>John J.</firstname><surname>Bromfield</surname><order>1</order></author><author><firstname>I. Martin</firstname><surname>Sheldon</surname><order>2</order></author><author><firstname>Martin</firstname><surname>Sheldon</surname><orcid>0000-0001-7902-5558</orcid><order>3</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2018-05-04T16:42:46.9414275 v2 15413 2013-08-09 Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1 ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2013-08-09 BMS Infections of the uterus or mammary gland with Gram-negative bacteria cause infertility in cattle, not only during disease but also for some time afterward. Even though these infections are in organs distant from the ovary, metritis and mastitis perturb antral follicle development and function in vivo. Although granulosa cells from antral follicles express toll-like receptor 4 (TLR4), and detect and mount an inflammatory response to lipopolysaccharide (LPS) from Gram-negative bacteria, it is not known whether LPS impacts preantral follicle development. The present study tested the hypothesis that LPS perturbs the development of primordial ovarian follicles. Exposure of bovine ovarian cortex ex vivo to LPS reduced the primordial follicle pool associated with increased primordial follicle activation. Ovarian cortex culture supernatants accumulated the inflammatory mediators IL-1beta, IL-6, and IL-8 in an LPS concentration-dependent manner. In addition, LPS exposure modulated key intracellular regulators of follicle activation with loss of the primordial follicle PTEN and cytoplasmic translocation of FOXO3. Acute exposure of mice in vivo to LPS also reduced the primordial follicle pool associated with increased follicle atresia. The increased follicle atresia was TLR4-dependent as Tlr4-deficient mice were insensitive to LPS-mediated follicle atresia. However, LPS did not affect the diameter of individually cultured bovine secondary follicles or their enclosed oocytes. In conclusion, LPS reduced the primordial ovarian follicle pool in the bovine ovarian cortex ex vivo and in the murine ovary in vivo. These observations provide an insight into how bacterial infections distant from the ovary have long term effects on fertility. Journal Article Biology of Reproduction 88 4 0006-3363 1529-7268 Ovary Infection Immunity 20 3 2013 2013-03-20 10.1095/biolreprod.112.106914 http://www.biolreprod.org/content/88/4/98.long COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2018-05-04T16:42:46.9414275 2013-08-09T08:41:55.0723263 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine John J. Bromfield 1 I. Martin Sheldon 2 Martin Sheldon 0000-0001-7902-5558 3
title Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
spellingShingle Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
Martin Sheldon
title_short Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
title_full Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
title_fullStr Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
title_full_unstemmed Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
title_sort Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1
author_id_str_mv ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author Martin Sheldon
author2 John J. Bromfield
I. Martin Sheldon
Martin Sheldon
format Journal article
container_title Biology of Reproduction
container_volume 88
container_issue 4
publishDate 2013
institution Swansea University
issn 0006-3363
1529-7268
doi_str_mv 10.1095/biolreprod.112.106914
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://www.biolreprod.org/content/88/4/98.long
document_store_str 0
active_str 0
description Infections of the uterus or mammary gland with Gram-negative bacteria cause infertility in cattle, not only during disease but also for some time afterward. Even though these infections are in organs distant from the ovary, metritis and mastitis perturb antral follicle development and function in vivo. Although granulosa cells from antral follicles express toll-like receptor 4 (TLR4), and detect and mount an inflammatory response to lipopolysaccharide (LPS) from Gram-negative bacteria, it is not known whether LPS impacts preantral follicle development. The present study tested the hypothesis that LPS perturbs the development of primordial ovarian follicles. Exposure of bovine ovarian cortex ex vivo to LPS reduced the primordial follicle pool associated with increased primordial follicle activation. Ovarian cortex culture supernatants accumulated the inflammatory mediators IL-1beta, IL-6, and IL-8 in an LPS concentration-dependent manner. In addition, LPS exposure modulated key intracellular regulators of follicle activation with loss of the primordial follicle PTEN and cytoplasmic translocation of FOXO3. Acute exposure of mice in vivo to LPS also reduced the primordial follicle pool associated with increased follicle atresia. The increased follicle atresia was TLR4-dependent as Tlr4-deficient mice were insensitive to LPS-mediated follicle atresia. However, LPS did not affect the diameter of individually cultured bovine secondary follicles or their enclosed oocytes. In conclusion, LPS reduced the primordial ovarian follicle pool in the bovine ovarian cortex ex vivo and in the murine ovary in vivo. These observations provide an insight into how bacterial infections distant from the ovary have long term effects on fertility.
published_date 2013-03-20T03:17:34Z
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