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Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse

, William Griffiths Orcid Logo, Yuqin Wang Orcid Logo

Biochemical and Biophysical Research Communications

Swansea University Authors: William Griffiths Orcid Logo, Yuqin Wang Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2014.01.153

Abstract

Oxysterols are oxidised forms of cholesterol or its precursors. In this study we utilised the cholesterol 24-hydroxylase knockout mouse (Cyp46a1-/-) to study the sterol and oxysterol content of brain. Despite a great reduction in the abundance of 24S-hydroxycholesterol, the dominant metabolite of ch...

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Published in: Biochemical and Biophysical Research Communications
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa17817
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first_indexed 2014-04-15T01:30:04Z
last_indexed 2018-02-09T04:51:50Z
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spelling 2014-04-14T16:43:57.4837239 v2 17817 2014-04-14 Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 2014-04-14 BMS Oxysterols are oxidised forms of cholesterol or its precursors. In this study we utilised the cholesterol 24-hydroxylase knockout mouse (Cyp46a1-/-) to study the sterol and oxysterol content of brain. Despite a great reduction in the abundance of 24S-hydroxycholesterol, the dominant metabolite of cholesterol in wild type brain, no other cholesterol metabolite was found to quantitatively replace this oxysterol in the Cyp46a1-/- mouse. Only minor amounts of other side-chain oxysterols including 22R-, 24R-, 25- and (25R),26-hydroxycholesterols were detected. In line with earlier studies, levels of cholesterol were similar in Cyp46a1-/- and wild type animals. However, the level of the cholesterol precursor, desomsterol, and its parallel metabolite formed via a shut of the mevalonate pathway, 24S,25-epoxycholesterol, were reduced in the Cyp46a1-/- mouse. The reduction in abundance of 24S,25-epoxycholesterol is interesting in light of a recent report indicating that this oxysterol promotes dopaminergic neurogenesis Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.01.153 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2014-04-14T16:43:57.4837239 2014-04-14T16:43:57.4837239 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine 1 William Griffiths 0000-0002-4129-6616 2 Yuqin Wang 0000-0002-3063-3066 3
title Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
spellingShingle Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
William Griffiths
Yuqin Wang
title_short Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
title_full Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
title_fullStr Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
title_full_unstemmed Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
title_sort Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
c92729b58622f9fdf6a0e7d8f4ce5081
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
author William Griffiths
Yuqin Wang
author2
William Griffiths
Yuqin Wang
format Journal article
container_title Biochemical and Biophysical Research Communications
publishDate 2014
institution Swansea University
doi_str_mv 10.1016/j.bbrc.2014.01.153
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Oxysterols are oxidised forms of cholesterol or its precursors. In this study we utilised the cholesterol 24-hydroxylase knockout mouse (Cyp46a1-/-) to study the sterol and oxysterol content of brain. Despite a great reduction in the abundance of 24S-hydroxycholesterol, the dominant metabolite of cholesterol in wild type brain, no other cholesterol metabolite was found to quantitatively replace this oxysterol in the Cyp46a1-/- mouse. Only minor amounts of other side-chain oxysterols including 22R-, 24R-, 25- and (25R),26-hydroxycholesterols were detected. In line with earlier studies, levels of cholesterol were similar in Cyp46a1-/- and wild type animals. However, the level of the cholesterol precursor, desomsterol, and its parallel metabolite formed via a shut of the mevalonate pathway, 24S,25-epoxycholesterol, were reduced in the Cyp46a1-/- mouse. The reduction in abundance of 24S,25-epoxycholesterol is interesting in light of a recent report indicating that this oxysterol promotes dopaminergic neurogenesis
published_date 2014-12-31T03:20:43Z
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score 10.998252

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