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24S,25-Epoxycholesterol in mouse and rat brain

Yuchen Wang, Kersti Karu, Anna Meljon, John Turton, Joyce L. Yau, Jonathan R. Seckl, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

Biochemical and Biophysical Research Communications

Swansea University Authors: Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2014.05.012

Abstract

24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high se...

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Published in: Biochemical and Biophysical Research Communications
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa17998
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Abstract: 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide
College: Swansea University Medical School