Journal article 1102 views
24S,25-Epoxycholesterol in mouse and rat brain
Yuchen Wang,
Kersti Karu,
Anna Meljon,
John Turton,
Joyce L. Yau,
Jonathan R. Seckl,
Yuqin Wang 
,
William Griffiths
,
William Griffiths
Biochemical and Biophysical Research Communications
Swansea University Authors:
Yuqin Wang , William Griffiths
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DOI (Published version): 10.1016/j.bbrc.2014.05.012
Abstract
24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high se...
| Published in: | Biochemical and Biophysical Research Communications |
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| Published: |
2014
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa17998 |
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| Abstract: |
24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide |
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| College: |
Faculty of Medicine, Health and Life Sciences |