Journal article 1207 views
24S,25-Epoxycholesterol in mouse and rat brain
Yuchen Wang,
Kersti Karu,
Anna Meljon,
John Turton,
Joyce L. Yau,
Jonathan R. Seckl,
Yuqin Wang
,
William Griffiths
Biochemical and Biophysical Research Communications
Swansea University Authors:
Yuqin Wang , William Griffiths
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DOI (Published version): 10.1016/j.bbrc.2014.05.012
Abstract
24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high se...
Published in: | Biochemical and Biophysical Research Communications |
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2014
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URI: | https://cronfa.swan.ac.uk/Record/cronfa17998 |
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<?xml version="1.0"?><rfc1807><datestamp>2014-05-22T10:09:20.9285633</datestamp><bib-version>v2</bib-version><id>17998</id><entry>2014-05-22</entry><title>24S,25-Epoxycholesterol in mouse and rat brain</title><swanseaauthors><author><sid>c92729b58622f9fdf6a0e7d8f4ce5081</sid><ORCID>0000-0002-3063-3066</ORCID><firstname>Yuqin</firstname><surname>Wang</surname><name>Yuqin Wang</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>3316b1d1b524be1831790933eed1c26e</sid><ORCID>0000-0002-4129-6616</ORCID><firstname>William</firstname><surname>Griffiths</surname><name>William Griffiths</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2014-05-22</date><deptcode>BMS</deptcode><abstract>24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide</abstract><type>Journal Article</type><journal>Biochemical and Biophysical Research Communications</journal><publisher/><keywords/><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2014</publishedYear><publishedDate>2014-12-31</publishedDate><doi>10.1016/j.bbrc.2014.05.012</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2014-05-22T10:09:20.9285633</lastEdited><Created>2014-05-22T10:09:20.9285633</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Yuchen</firstname><surname>Wang</surname><order>1</order></author><author><firstname>Kersti</firstname><surname>Karu</surname><order>2</order></author><author><firstname>Anna</firstname><surname>Meljon</surname><order>3</order></author><author><firstname>John</firstname><surname>Turton</surname><order>4</order></author><author><firstname>Joyce L.</firstname><surname>Yau</surname><order>5</order></author><author><firstname>Jonathan R.</firstname><surname>Seckl</surname><order>6</order></author><author><firstname>Yuqin</firstname><surname>Wang</surname><orcid>0000-0002-3063-3066</orcid><order>7</order></author><author><firstname>William</firstname><surname>Griffiths</surname><orcid>0000-0002-4129-6616</orcid><order>8</order></author></authors><documents/><OutputDurs/></rfc1807> |
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2014-05-22T10:09:20.9285633 v2 17998 2014-05-22 24S,25-Epoxycholesterol in mouse and rat brain c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2014-05-22 BMS 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.05.012 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2014-05-22T10:09:20.9285633 2014-05-22T10:09:20.9285633 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Yuchen Wang 1 Kersti Karu 2 Anna Meljon 3 John Turton 4 Joyce L. Yau 5 Jonathan R. Seckl 6 Yuqin Wang 0000-0002-3063-3066 7 William Griffiths 0000-0002-4129-6616 8 |
title |
24S,25-Epoxycholesterol in mouse and rat brain |
spellingShingle |
24S,25-Epoxycholesterol in mouse and rat brain Yuqin Wang William Griffiths |
title_short |
24S,25-Epoxycholesterol in mouse and rat brain |
title_full |
24S,25-Epoxycholesterol in mouse and rat brain |
title_fullStr |
24S,25-Epoxycholesterol in mouse and rat brain |
title_full_unstemmed |
24S,25-Epoxycholesterol in mouse and rat brain |
title_sort |
24S,25-Epoxycholesterol in mouse and rat brain |
author_id_str_mv |
c92729b58622f9fdf6a0e7d8f4ce5081 3316b1d1b524be1831790933eed1c26e |
author_id_fullname_str_mv |
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang 3316b1d1b524be1831790933eed1c26e_***_William Griffiths |
author |
Yuqin Wang William Griffiths |
author2 |
Yuchen Wang Kersti Karu Anna Meljon John Turton Joyce L. Yau Jonathan R. Seckl Yuqin Wang William Griffiths |
format |
Journal article |
container_title |
Biochemical and Biophysical Research Communications |
publishDate |
2014 |
institution |
Swansea University |
doi_str_mv |
10.1016/j.bbrc.2014.05.012 |
college_str |
Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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0 |
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description |
24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide |
published_date |
2014-12-31T03:20:59Z |
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1763750611872907264 |
score |
11.021648 |