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24S,25-Epoxycholesterol in mouse and rat brain

Yuchen Wang, Kersti Karu, Anna Meljon, John Turton, Joyce L. Yau, Jonathan R. Seckl, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

Biochemical and Biophysical Research Communications

Swansea University Authors: Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2014.05.012

Abstract

24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high se...

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Published in: Biochemical and Biophysical Research Communications
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa17998
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first_indexed 2014-05-29T10:24:50Z
last_indexed 2018-02-09T04:52:10Z
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spelling 2014-05-22T10:09:20.9285633 v2 17998 2014-05-22 24S,25-Epoxycholesterol in mouse and rat brain c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2014-05-22 BMS 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.05.012 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2014-05-22T10:09:20.9285633 2014-05-22T10:09:20.9285633 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Yuchen Wang 1 Kersti Karu 2 Anna Meljon 3 John Turton 4 Joyce L. Yau 5 Jonathan R. Seckl 6 Yuqin Wang 0000-0002-3063-3066 7 William Griffiths 0000-0002-4129-6616 8
title 24S,25-Epoxycholesterol in mouse and rat brain
spellingShingle 24S,25-Epoxycholesterol in mouse and rat brain
Yuqin Wang
William Griffiths
title_short 24S,25-Epoxycholesterol in mouse and rat brain
title_full 24S,25-Epoxycholesterol in mouse and rat brain
title_fullStr 24S,25-Epoxycholesterol in mouse and rat brain
title_full_unstemmed 24S,25-Epoxycholesterol in mouse and rat brain
title_sort 24S,25-Epoxycholesterol in mouse and rat brain
author_id_str_mv c92729b58622f9fdf6a0e7d8f4ce5081
3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author Yuqin Wang
William Griffiths
author2 Yuchen Wang
Kersti Karu
Anna Meljon
John Turton
Joyce L. Yau
Jonathan R. Seckl
Yuqin Wang
William Griffiths
format Journal article
container_title Biochemical and Biophysical Research Communications
publishDate 2014
institution Swansea University
doi_str_mv 10.1016/j.bbrc.2014.05.012
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide
published_date 2014-12-31T03:21:05Z
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score 10.927374