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Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment

Enrico Andreoli, Rei Suzuki, Alvin W. Orbaek, Manoop S. Bhutani, Robert H. Hauge, Wade Adams, Jason B. Fleming, Andrew Barron Orcid Logo, Alvin Orbaek White Orcid Logo

Journal of Materials Chemistry B, Volume: 2, Issue: 29, Pages: 4740 - 4747

Swansea University Authors: Andrew Barron Orcid Logo, Alvin Orbaek White Orcid Logo

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DOI (Published version): 10.1039/C4TB00778F

Abstract

High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then t...

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Published in: Journal of Materials Chemistry B
ISSN: 2050-750X 2050-7518
Published: 2014
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URI: https://cronfa.swan.ac.uk/Record/cronfa22210
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Abstract: High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then tested for cytotoxicity both in vitro (HPNE and BxPC3 pancreatic cell lines) and in vivo (BxPC3 xenografts from nude mice) to establish that functionalization with lower molecular weight PEI (600 and 1800 Da) achieved higher cell viability in MTT assay. A shortened version of the nanotubes, PEI(1800)-cut-SWNT (1800 Da branched PEI), was also prepared and tested for cellular internalization in the BxPC3 adenocarcinoma cell line. Laser confocal imaging of the cells after incubation in the presence of RhoB-PEI(1800)-cut-SWNT (covalently labelled with rhodamine B) indicates that the PEI(1800)-cut-SWNTs can reach both the cytoplasm and nucleus of pancreatic cancer cells.
Issue: 29
Start Page: 4740
End Page: 4747

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