Journal article 1319 views
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
Journal of Materials Chemistry B, Volume: 2, Issue: 29, Pages: 4740 - 4747
Swansea University Authors:
Andrew Barron , Alvin Orbaek White
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1039/C4TB00778F
Abstract
High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then t...
Published in: | Journal of Materials Chemistry B |
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ISSN: | 2050-750X 2050-7518 |
Published: |
2014
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URI: | https://cronfa.swan.ac.uk/Record/cronfa22210 |
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<?xml version="1.0"?><rfc1807><datestamp>2020-06-10T14:41:19.7031581</datestamp><bib-version>v2</bib-version><id>22210</id><entry>2015-06-26</entry><title>Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment</title><swanseaauthors><author><sid>92e452f20936d688d36f91c78574241d</sid><ORCID>0000-0002-2018-8288</ORCID><firstname>Andrew</firstname><surname>Barron</surname><name>Andrew Barron</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>8414a23650d4403fdfe1a735dbd2e24e</sid><ORCID>0000-0001-6338-5970</ORCID><firstname>Alvin</firstname><surname>Orbaek White</surname><name>Alvin Orbaek White</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2015-06-26</date><deptcode>CHEG</deptcode><abstract>High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then tested for cytotoxicity both in vitro (HPNE and BxPC3 pancreatic cell lines) and in vivo (BxPC3 xenografts from nude mice) to establish that functionalization with lower molecular weight PEI (600 and 1800 Da) achieved higher cell viability in MTT assay. A shortened version of the nanotubes, PEI(1800)-cut-SWNT (1800 Da branched PEI), was also prepared and tested for cellular internalization in the BxPC3 adenocarcinoma cell line. Laser confocal imaging of the cells after incubation in the presence of RhoB-PEI(1800)-cut-SWNT (covalently labelled with rhodamine B) indicates that the PEI(1800)-cut-SWNTs can reach both the cytoplasm and nucleus of pancreatic cancer cells.</abstract><type>Journal Article</type><journal>Journal of Materials Chemistry B</journal><volume>2</volume><journalNumber>29</journalNumber><paginationStart>4740</paginationStart><paginationEnd>4747</paginationEnd><publisher/><issnPrint>2050-750X</issnPrint><issnElectronic>2050-7518</issnElectronic><keywords/><publishedDay>7</publishedDay><publishedMonth>8</publishedMonth><publishedYear>2014</publishedYear><publishedDate>2014-08-07</publishedDate><doi>10.1039/C4TB00778F</doi><url>http://pubs.rsc.org/en/Content/ArticleLanding/2014/TB/c4tb00778f#!divAbstract</url><notes/><college>COLLEGE NANME</college><department>Chemical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>CHEG</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-06-10T14:41:19.7031581</lastEdited><Created>2015-06-26T16:18:21.9269522</Created><authors><author><firstname>Enrico</firstname><surname>Andreoli</surname><order>1</order></author><author><firstname>Rei</firstname><surname>Suzuki</surname><order>2</order></author><author><firstname>Alvin W.</firstname><surname>Orbaek</surname><order>3</order></author><author><firstname>Manoop S.</firstname><surname>Bhutani</surname><order>4</order></author><author><firstname>Robert H.</firstname><surname>Hauge</surname><order>5</order></author><author><firstname>Wade</firstname><surname>Adams</surname><order>6</order></author><author><firstname>Jason B.</firstname><surname>Fleming</surname><order>7</order></author><author><firstname>Andrew</firstname><surname>Barron</surname><orcid>0000-0002-2018-8288</orcid><order>8</order></author><author><firstname>Alvin</firstname><surname>Orbaek White</surname><orcid>0000-0001-6338-5970</orcid><order>9</order></author></authors><documents/><OutputDurs/></rfc1807> |
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2020-06-10T14:41:19.7031581 v2 22210 2015-06-26 Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment 92e452f20936d688d36f91c78574241d 0000-0002-2018-8288 Andrew Barron Andrew Barron true false 8414a23650d4403fdfe1a735dbd2e24e 0000-0001-6338-5970 Alvin Orbaek White Alvin Orbaek White true false 2015-06-26 CHEG High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then tested for cytotoxicity both in vitro (HPNE and BxPC3 pancreatic cell lines) and in vivo (BxPC3 xenografts from nude mice) to establish that functionalization with lower molecular weight PEI (600 and 1800 Da) achieved higher cell viability in MTT assay. A shortened version of the nanotubes, PEI(1800)-cut-SWNT (1800 Da branched PEI), was also prepared and tested for cellular internalization in the BxPC3 adenocarcinoma cell line. Laser confocal imaging of the cells after incubation in the presence of RhoB-PEI(1800)-cut-SWNT (covalently labelled with rhodamine B) indicates that the PEI(1800)-cut-SWNTs can reach both the cytoplasm and nucleus of pancreatic cancer cells. Journal Article Journal of Materials Chemistry B 2 29 4740 4747 2050-750X 2050-7518 7 8 2014 2014-08-07 10.1039/C4TB00778F http://pubs.rsc.org/en/Content/ArticleLanding/2014/TB/c4tb00778f#!divAbstract COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University 2020-06-10T14:41:19.7031581 2015-06-26T16:18:21.9269522 Enrico Andreoli 1 Rei Suzuki 2 Alvin W. Orbaek 3 Manoop S. Bhutani 4 Robert H. Hauge 5 Wade Adams 6 Jason B. Fleming 7 Andrew Barron 0000-0002-2018-8288 8 Alvin Orbaek White 0000-0001-6338-5970 9 |
title |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment |
spellingShingle |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment Andrew Barron Alvin Orbaek White |
title_short |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment |
title_full |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment |
title_fullStr |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment |
title_full_unstemmed |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment |
title_sort |
Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment |
author_id_str_mv |
92e452f20936d688d36f91c78574241d 8414a23650d4403fdfe1a735dbd2e24e |
author_id_fullname_str_mv |
92e452f20936d688d36f91c78574241d_***_Andrew Barron 8414a23650d4403fdfe1a735dbd2e24e_***_Alvin Orbaek White |
author |
Andrew Barron Alvin Orbaek White |
author2 |
Enrico Andreoli Rei Suzuki Alvin W. Orbaek Manoop S. Bhutani Robert H. Hauge Wade Adams Jason B. Fleming Andrew Barron Alvin Orbaek White |
format |
Journal article |
container_title |
Journal of Materials Chemistry B |
container_volume |
2 |
container_issue |
29 |
container_start_page |
4740 |
publishDate |
2014 |
institution |
Swansea University |
issn |
2050-750X 2050-7518 |
doi_str_mv |
10.1039/C4TB00778F |
url |
http://pubs.rsc.org/en/Content/ArticleLanding/2014/TB/c4tb00778f#!divAbstract |
document_store_str |
0 |
active_str |
0 |
description |
High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then tested for cytotoxicity both in vitro (HPNE and BxPC3 pancreatic cell lines) and in vivo (BxPC3 xenografts from nude mice) to establish that functionalization with lower molecular weight PEI (600 and 1800 Da) achieved higher cell viability in MTT assay. A shortened version of the nanotubes, PEI(1800)-cut-SWNT (1800 Da branched PEI), was also prepared and tested for cellular internalization in the BxPC3 adenocarcinoma cell line. Laser confocal imaging of the cells after incubation in the presence of RhoB-PEI(1800)-cut-SWNT (covalently labelled with rhodamine B) indicates that the PEI(1800)-cut-SWNTs can reach both the cytoplasm and nucleus of pancreatic cancer cells. |
published_date |
2014-08-07T03:26:25Z |
_version_ |
1763750954567467008 |
score |
11.021648 |