Conference Paper/Proceeding/Abstract 299 views
Detection of monoclonal antibodies using chemically modified graphite substrates
Z Tehrani,
O J Guy,
A Castaing,
S H Doak,
Zari Tehrani 
2010 IEEE Sensors, Pages: 428 - 431
Swansea University Author:
Zari Tehrani
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1109/ICSENS.2010.5690189
Abstract
This paper presents a novel sensor device based on chemically modified Highly Ordered Pyrolytic Graphite (HOPG). Biosensor diagnostics based on bio-functionalised semiconductor devices are an important development in ultrasensitive sensors for early detection of disease biomarkers. Electrochemical d...
| Published in: | 2010 IEEE Sensors |
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| Published: |
2010
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa31400 |
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| Abstract: |
This paper presents a novel sensor device based on chemically modified Highly Ordered Pyrolytic Graphite (HOPG). Biosensor diagnostics based on bio-functionalised semiconductor devices are an important development in ultrasensitive sensors for early detection of disease biomarkers. Electrochemical devices using chemically modified graphite (CMG) channels are excellent candidates for nano-biosensors [1]. By attaching aniline to HOPG, via coupling with an aryl diazonium salt, the amino group of the aniline molecule has been used to graft antibodies - (1) targeted against beta-actin and (2) targeted against 8-hydroxydeoxyguanosine (8-OHdG) - onto the HOPG surface. Antibody attachment to graphitic surfaces has been verified using Laser Scanning Confocal Microscopy (LSCM) to detect attached quantum-dot labeled antibodies. Furthermore, the current-voltage characteristics of virgin and chemically modified HOPG surfaces have been used to detect the presence of antibodies at nM concentrations. |
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| College: |
Faculty of Science and Engineering |
| Start Page: |
428 |
| End Page: |
431 |