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Conference Paper/Proceeding/Abstract 299 views

Detection of monoclonal antibodies using chemically modified graphite substrates

Z Tehrani, O J Guy, A Castaing, S H Doak, Zari Tehrani Orcid Logo

2010 IEEE Sensors, Pages: 428 - 431

Swansea University Author: Zari Tehrani Orcid Logo

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DOI (Published version): 10.1109/ICSENS.2010.5690189

Abstract

This paper presents a novel sensor device based on chemically modified Highly Ordered Pyrolytic Graphite (HOPG). Biosensor diagnostics based on bio-functionalised semiconductor devices are an important development in ultrasensitive sensors for early detection of disease biomarkers. Electrochemical d...

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Published in: 2010 IEEE Sensors
Published: 2010
URI: https://cronfa.swan.ac.uk/Record/cronfa31400
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first_indexed 2016-12-08T15:06:22Z
last_indexed 2018-02-09T05:18:16Z
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spelling 2016-12-08T11:40:27.3545012 v2 31400 2016-12-08 Detection of monoclonal antibodies using chemically modified graphite substrates fd8e614b01086804c80fbafa6fa6aaf5 0000-0002-5069-7921 Zari Tehrani Zari Tehrani true false 2016-12-08 CHEG This paper presents a novel sensor device based on chemically modified Highly Ordered Pyrolytic Graphite (HOPG). Biosensor diagnostics based on bio-functionalised semiconductor devices are an important development in ultrasensitive sensors for early detection of disease biomarkers. Electrochemical devices using chemically modified graphite (CMG) channels are excellent candidates for nano-biosensors [1]. By attaching aniline to HOPG, via coupling with an aryl diazonium salt, the amino group of the aniline molecule has been used to graft antibodies - (1) targeted against beta-actin and (2) targeted against 8-hydroxydeoxyguanosine (8-OHdG) - onto the HOPG surface. Antibody attachment to graphitic surfaces has been verified using Laser Scanning Confocal Microscopy (LSCM) to detect attached quantum-dot labeled antibodies. Furthermore, the current-voltage characteristics of virgin and chemically modified HOPG surfaces have been used to detect the presence of antibodies at nM concentrations. Conference Paper/Proceeding/Abstract 2010 IEEE Sensors 428 431 31 12 2010 2010-12-31 10.1109/ICSENS.2010.5690189 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University 2016-12-08T11:40:27.3545012 2016-12-08T11:39:45.9984510 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Z Tehrani 1 O J Guy 2 A Castaing 3 S H Doak 4 Zari Tehrani 0000-0002-5069-7921 5
title Detection of monoclonal antibodies using chemically modified graphite substrates
spellingShingle Detection of monoclonal antibodies using chemically modified graphite substrates
Zari Tehrani
title_short Detection of monoclonal antibodies using chemically modified graphite substrates
title_full Detection of monoclonal antibodies using chemically modified graphite substrates
title_fullStr Detection of monoclonal antibodies using chemically modified graphite substrates
title_full_unstemmed Detection of monoclonal antibodies using chemically modified graphite substrates
title_sort Detection of monoclonal antibodies using chemically modified graphite substrates
author_id_str_mv fd8e614b01086804c80fbafa6fa6aaf5
author_id_fullname_str_mv fd8e614b01086804c80fbafa6fa6aaf5_***_Zari Tehrani
author Zari Tehrani
author2 Z Tehrani
O J Guy
A Castaing
S H Doak
Zari Tehrani
format Conference Paper/Proceeding/Abstract
container_title 2010 IEEE Sensors
container_start_page 428
publishDate 2010
institution Swansea University
doi_str_mv 10.1109/ICSENS.2010.5690189
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 0
active_str 0
description This paper presents a novel sensor device based on chemically modified Highly Ordered Pyrolytic Graphite (HOPG). Biosensor diagnostics based on bio-functionalised semiconductor devices are an important development in ultrasensitive sensors for early detection of disease biomarkers. Electrochemical devices using chemically modified graphite (CMG) channels are excellent candidates for nano-biosensors [1]. By attaching aniline to HOPG, via coupling with an aryl diazonium salt, the amino group of the aniline molecule has been used to graft antibodies - (1) targeted against beta-actin and (2) targeted against 8-hydroxydeoxyguanosine (8-OHdG) - onto the HOPG surface. Antibody attachment to graphitic surfaces has been verified using Laser Scanning Confocal Microscopy (LSCM) to detect attached quantum-dot labeled antibodies. Furthermore, the current-voltage characteristics of virgin and chemically modified HOPG surfaces have been used to detect the presence of antibodies at nM concentrations.
published_date 2010-12-31T03:38:21Z
_version_ 1763751705296502784
score 11.016235

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