Journal article 1120 views
Towards a minimally invasive device for beta-lactam monitoring in humans
Timothy Miles Rawson,
Sanjiv Sharma 
,
Pantelis Georgiou,
Alison Holmes,
Anthony Cass,
Danny O'Hare
,
Pantelis Georgiou,
Alison Holmes,
Anthony Cass,
Danny O'Hare
Electrochemistry Communications, Volume: 82, Pages: 1 - 5
Swansea University Author:
Sanjiv Sharma
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1016/j.elecom.2017.07.011
Abstract
Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human intersti...
| Published in: | Electrochemistry Communications |
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| ISSN: | 1388-2481 |
| Published: |
2017
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa36437 |
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| Abstract: |
Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (± 1SD) = 0.0044 ± 0.0013 M and mean slope function of log(concentration plots) 29 ± 1.80 mV/decade (r2 = 0.933). Response was reproducible after 28 days storage at 4 °C. In artificial ISF, the sensors response was Km (± 1SD) = 0.0077 ± 0.0187 M and a slope function of 34 ± 1.85 mv/decade (r2 = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor. |
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| Keywords: |
Beta-lactam antibiotic monitoring, Minimally invasive, Continuous monitoring, Antibiotic resistance |
| College: |
Faculty of Science and Engineering |
| Start Page: |
1 |
| End Page: |
5 |