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Towards a minimally invasive device for beta-lactam monitoring in humans

Timothy Miles Rawson, Sanjiv Sharma Orcid Logo, Pantelis Georgiou, Alison Holmes, Anthony Cass, Danny O'Hare

Electrochemistry Communications, Volume: 82, Pages: 1 - 5

Swansea University Author: Sanjiv Sharma Orcid Logo

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DOI (Published version): 10.1016/j.elecom.2017.07.011

Abstract

Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human intersti...

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Published in: Electrochemistry Communications
ISSN: 1388-2481
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa36437
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first_indexed 2017-11-01T19:54:03Z
last_indexed 2020-06-19T18:50:02Z
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2020-06-19T14:31:13.5663906</datestamp><bib-version>v2</bib-version><id>36437</id><entry>2017-11-01</entry><title>Towards a minimally invasive device for beta-lactam monitoring in humans</title><swanseaauthors><author><sid>b6b7506358522f607b171ec9c94757b7</sid><ORCID>0000-0003-3828-737X</ORCID><firstname>Sanjiv</firstname><surname>Sharma</surname><name>Sanjiv Sharma</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-11-01</date><deptcode>MEDE</deptcode><abstract>Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (&#xB1; 1SD) = 0.0044 &#xB1; 0.0013 M and mean slope function of log(concentration plots) 29 &#xB1; 1.80 mV/decade (r2 = 0.933). Response was reproducible after 28 days storage at 4 &#xB0;C. In artificial ISF, the sensors response was Km (&#xB1; 1SD) = 0.0077 &#xB1; 0.0187 M and a slope function of 34 &#xB1; 1.85 mv/decade (r2 = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor.</abstract><type>Journal Article</type><journal>Electrochemistry Communications</journal><volume>82</volume><paginationStart>1</paginationStart><paginationEnd>5</paginationEnd><publisher/><issnPrint>1388-2481</issnPrint><keywords>Beta-lactam antibiotic monitoring, Minimally invasive, Continuous monitoring, Antibiotic resistance</keywords><publishedDay>1</publishedDay><publishedMonth>9</publishedMonth><publishedYear>2017</publishedYear><publishedDate>2017-09-01</publishedDate><doi>10.1016/j.elecom.2017.07.011</doi><url>https://spiral.imperial.ac.uk/handle/10044/1/52776</url><notes/><college>COLLEGE NANME</college><department>Biomedical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDE</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-06-19T14:31:13.5663906</lastEdited><Created>2017-11-01T16:24:59.7808949</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Biomedical Engineering</level></path><authors><author><firstname>Timothy Miles</firstname><surname>Rawson</surname><order>1</order></author><author><firstname>Sanjiv</firstname><surname>Sharma</surname><orcid>0000-0003-3828-737X</orcid><order>2</order></author><author><firstname>Pantelis</firstname><surname>Georgiou</surname><order>3</order></author><author><firstname>Alison</firstname><surname>Holmes</surname><order>4</order></author><author><firstname>Anthony</firstname><surname>Cass</surname><order>5</order></author><author><firstname>Danny</firstname><surname>O'Hare</surname><order>6</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2020-06-19T14:31:13.5663906 v2 36437 2017-11-01 Towards a minimally invasive device for beta-lactam monitoring in humans b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2017-11-01 MEDE Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (± 1SD) = 0.0044 ± 0.0013 M and mean slope function of log(concentration plots) 29 ± 1.80 mV/decade (r2 = 0.933). Response was reproducible after 28 days storage at 4 °C. In artificial ISF, the sensors response was Km (± 1SD) = 0.0077 ± 0.0187 M and a slope function of 34 ± 1.85 mv/decade (r2 = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor. Journal Article Electrochemistry Communications 82 1 5 1388-2481 Beta-lactam antibiotic monitoring, Minimally invasive, Continuous monitoring, Antibiotic resistance 1 9 2017 2017-09-01 10.1016/j.elecom.2017.07.011 https://spiral.imperial.ac.uk/handle/10044/1/52776 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-06-19T14:31:13.5663906 2017-11-01T16:24:59.7808949 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Timothy Miles Rawson 1 Sanjiv Sharma 0000-0003-3828-737X 2 Pantelis Georgiou 3 Alison Holmes 4 Anthony Cass 5 Danny O'Hare 6
title Towards a minimally invasive device for beta-lactam monitoring in humans
spellingShingle Towards a minimally invasive device for beta-lactam monitoring in humans
Sanjiv Sharma
title_short Towards a minimally invasive device for beta-lactam monitoring in humans
title_full Towards a minimally invasive device for beta-lactam monitoring in humans
title_fullStr Towards a minimally invasive device for beta-lactam monitoring in humans
title_full_unstemmed Towards a minimally invasive device for beta-lactam monitoring in humans
title_sort Towards a minimally invasive device for beta-lactam monitoring in humans
author_id_str_mv b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Sanjiv Sharma
author2 Timothy Miles Rawson
Sanjiv Sharma
Pantelis Georgiou
Alison Holmes
Anthony Cass
Danny O'Hare
format Journal article
container_title Electrochemistry Communications
container_volume 82
container_start_page 1
publishDate 2017
institution Swansea University
issn 1388-2481
doi_str_mv 10.1016/j.elecom.2017.07.011
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
url https://spiral.imperial.ac.uk/handle/10044/1/52776
document_store_str 0
active_str 0
description Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (± 1SD) = 0.0044 ± 0.0013 M and mean slope function of log(concentration plots) 29 ± 1.80 mV/decade (r2 = 0.933). Response was reproducible after 28 days storage at 4 °C. In artificial ISF, the sensors response was Km (± 1SD) = 0.0077 ± 0.0187 M and a slope function of 34 ± 1.85 mv/decade (r2 = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor.
published_date 2017-09-01T03:45:34Z
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score 11.01628

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