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Protein-responsive polymers for point-of-care detection of cardiac biomarker / Felismina T.C. Moreira; Sanjiv Sharma; Rosa A.F. Dutra; João P.C. Noronha; Anthony E.G. Cass; M. Goreti F. Sales

Sensors and Actuators B: Chemical, Volume: 196, Pages: 123 - 132

Swansea University Author: Sanjiv, Sharma

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Abstract

This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made responsive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surfac...

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Published in: Sensors and Actuators B: Chemical
ISSN: 0925-4005
Published: 2014
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URI: https://cronfa.swan.ac.uk/Record/cronfa36442
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2020-10-07T13:20:20.1151052</datestamp><bib-version>v2</bib-version><id>36442</id><entry>2017-11-01</entry><title>Protein-responsive polymers for point-of-care detection of cardiac biomarker</title><swanseaauthors><author><sid>b6b7506358522f607b171ec9c94757b7</sid><ORCID>0000-0003-3828-737X</ORCID><firstname>Sanjiv</firstname><surname>Sharma</surname><name>Sanjiv Sharma</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-11-01</date><deptcode>EEN</deptcode><abstract>This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made responsive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the material to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The devices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 &#x3BC;g/mL, respectively, with detection limits of 1.5 and 0.8 &#x3BC;g/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivity and selectivity.</abstract><type>Journal Article</type><journal>Sensors and Actuators B: Chemical</journal><volume>196</volume><paginationStart>123</paginationStart><paginationEnd>132</paginationEnd><publisher/><issnPrint>0925-4005</issnPrint><keywords>Biomimetic antibodies, Screen-printed electrodes, Molecular imprinting, Electropolymerization, Myoglobin, Biosensor</keywords><publishedDay>30</publishedDay><publishedMonth>6</publishedMonth><publishedYear>2014</publishedYear><publishedDate>2014-06-30</publishedDate><doi>10.1016/j.snb.2014.01.038</doi><url>http://www.sciencedirect.com/science/article/pii/S0925400514000550</url><notes/><college>COLLEGE NANME</college><department>Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>EEN</DepartmentCode><institution>Swansea University</institution><lastEdited>2020-10-07T13:20:20.1151052</lastEdited><Created>2017-11-01T16:25:14.4762496</Created><path><level id="1"/><level id="2"/></path><authors><author><firstname>Felismina T.C.</firstname><surname>Moreira</surname><order>1</order></author><author><firstname>Sanjiv</firstname><surname>Sharma</surname><orcid>0000-0003-3828-737X</orcid><order>2</order></author><author><firstname>Rosa A.F.</firstname><surname>Dutra</surname><order>3</order></author><author><firstname>Jo&#xE3;o P.C.</firstname><surname>Noronha</surname><order>4</order></author><author><firstname>Anthony E.G.</firstname><surname>Cass</surname><order>5</order></author><author><firstname>M. Goreti F.</firstname><surname>Sales</surname><order>6</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2020-10-07T13:20:20.1151052 v2 36442 2017-11-01 Protein-responsive polymers for point-of-care detection of cardiac biomarker b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2017-11-01 EEN This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made responsive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the material to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The devices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 μg/mL, respectively, with detection limits of 1.5 and 0.8 μg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivity and selectivity. Journal Article Sensors and Actuators B: Chemical 196 123 132 0925-4005 Biomimetic antibodies, Screen-printed electrodes, Molecular imprinting, Electropolymerization, Myoglobin, Biosensor 30 6 2014 2014-06-30 10.1016/j.snb.2014.01.038 http://www.sciencedirect.com/science/article/pii/S0925400514000550 COLLEGE NANME Engineering COLLEGE CODE EEN Swansea University 2020-10-07T13:20:20.1151052 2017-11-01T16:25:14.4762496 Felismina T.C. Moreira 1 Sanjiv Sharma 0000-0003-3828-737X 2 Rosa A.F. Dutra 3 João P.C. Noronha 4 Anthony E.G. Cass 5 M. Goreti F. Sales 6
title Protein-responsive polymers for point-of-care detection of cardiac biomarker
spellingShingle Protein-responsive polymers for point-of-care detection of cardiac biomarker
Sanjiv, Sharma
title_short Protein-responsive polymers for point-of-care detection of cardiac biomarker
title_full Protein-responsive polymers for point-of-care detection of cardiac biomarker
title_fullStr Protein-responsive polymers for point-of-care detection of cardiac biomarker
title_full_unstemmed Protein-responsive polymers for point-of-care detection of cardiac biomarker
title_sort Protein-responsive polymers for point-of-care detection of cardiac biomarker
author_id_str_mv b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv b6b7506358522f607b171ec9c94757b7_***_Sanjiv, Sharma
author Sanjiv, Sharma
author2 Felismina T.C. Moreira
Sanjiv Sharma
Rosa A.F. Dutra
João P.C. Noronha
Anthony E.G. Cass
M. Goreti F. Sales
format Journal article
container_title Sensors and Actuators B: Chemical
container_volume 196
container_start_page 123
publishDate 2014
institution Swansea University
issn 0925-4005
doi_str_mv 10.1016/j.snb.2014.01.038
url http://www.sciencedirect.com/science/article/pii/S0925400514000550
document_store_str 0
active_str 0
description This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made responsive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the material to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The devices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 μg/mL, respectively, with detection limits of 1.5 and 0.8 μg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivity and selectivity.
published_date 2014-06-30T03:57:27Z
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score 10.788329