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In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices

Jonathan Widdowson, Alex J. Picton, Valerie Vince, Chris J. Wright, Andrew Mearns-Spragg, Christopher Wright Orcid Logo

Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume: 106, Issue: 4, Pages: 1524 - 1533

Swansea University Authors: Jonathan Widdowson, Christopher Wright Orcid Logo

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DOI (Published version): 10.1002/jbm.b.33959

Abstract

Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vector...

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Published in: Journal of Biomedical Materials Research Part B: Applied Biomaterials
ISSN: 1552-4973
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa39891
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spelling 2019-07-12T15:55:32.0977834 v2 39891 2018-05-03 In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices 768d398f0d415f52c20b0b1deb93ce38 Jonathan Widdowson Jonathan Widdowson true false 235e125ac3463e2ee7fc98604bf879ce 0000-0003-2375-8159 Christopher Wright Christopher Wright true false 2018-05-03 FGSEN Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges. The scaffolds were prepared and examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and scanning electron microscopy (SEM). The samples were implanted in adult male Wistar rats for in vivo experimentation. Both crosslinked and uncrosslinked jellyfish collagen sponges showed a significant reduction in histopathology scores over the course of the study, whereas the bovine collagen sponge scores were not significantly reduced. Both jellyfish collagen sponges and the bovine sponge were tolerated well by the hosts, and a recovery was visible in all samples, suggesting that R. pulmo jellyfish‐derived collagen could offer compelling biocompatibility with wound healing applications. We also demonstrate that noncrosslinked samples could be safer with better resorption times than crosslinked samples. Journal Article Journal of Biomedical Materials Research Part B: Applied Biomaterials 106 4 1524 1533 1552-4973 jellyfish collagen, in vivo, regenerative medicine, collagen implantation, biomaterials 16 4 2018 2018-04-16 10.1002/jbm.b.33959 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2019-07-12T15:55:32.0977834 2018-05-03T11:10:51.2880650 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Jonathan Widdowson 1 Alex J. Picton 2 Valerie Vince 3 Chris J. Wright 4 Andrew Mearns-Spragg 5 Christopher Wright 0000-0003-2375-8159 6 0039891-15052018141954.pdf widdowson2017v2.pdf 2018-05-15T14:19:54.5400000 Output 708773 application/pdf Version of Record true 2018-05-15T00:00:00.0000000 true eng
title In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
spellingShingle In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
Jonathan Widdowson
Christopher Wright
title_short In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
title_full In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
title_fullStr In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
title_full_unstemmed In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
title_sort In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
author_id_str_mv 768d398f0d415f52c20b0b1deb93ce38
235e125ac3463e2ee7fc98604bf879ce
author_id_fullname_str_mv 768d398f0d415f52c20b0b1deb93ce38_***_Jonathan Widdowson
235e125ac3463e2ee7fc98604bf879ce_***_Christopher Wright
author Jonathan Widdowson
Christopher Wright
author2 Jonathan Widdowson
Alex J. Picton
Valerie Vince
Chris J. Wright
Andrew Mearns-Spragg
Christopher Wright
format Journal article
container_title Journal of Biomedical Materials Research Part B: Applied Biomaterials
container_volume 106
container_issue 4
container_start_page 1524
publishDate 2018
institution Swansea University
issn 1552-4973
doi_str_mv 10.1002/jbm.b.33959
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges. The scaffolds were prepared and examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and scanning electron microscopy (SEM). The samples were implanted in adult male Wistar rats for in vivo experimentation. Both crosslinked and uncrosslinked jellyfish collagen sponges showed a significant reduction in histopathology scores over the course of the study, whereas the bovine collagen sponge scores were not significantly reduced. Both jellyfish collagen sponges and the bovine sponge were tolerated well by the hosts, and a recovery was visible in all samples, suggesting that R. pulmo jellyfish‐derived collagen could offer compelling biocompatibility with wound healing applications. We also demonstrate that noncrosslinked samples could be safer with better resorption times than crosslinked samples.
published_date 2018-04-16T03:50:44Z
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