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In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume: 106, Issue: 4, Pages: 1524 - 1533
Swansea University Authors: Jonathan Widdowson, Christopher Wright
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DOI (Published version): 10.1002/jbm.b.33959
Abstract
Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vector...
Published in: | Journal of Biomedical Materials Research Part B: Applied Biomaterials |
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ISSN: | 1552-4973 |
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2018
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URI: | https://cronfa.swan.ac.uk/Record/cronfa39891 |
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2019-07-12T15:55:32.0977834 v2 39891 2018-05-03 In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices 768d398f0d415f52c20b0b1deb93ce38 Jonathan Widdowson Jonathan Widdowson true false 235e125ac3463e2ee7fc98604bf879ce 0000-0003-2375-8159 Christopher Wright Christopher Wright true false 2018-05-03 FGSEN Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges. The scaffolds were prepared and examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and scanning electron microscopy (SEM). The samples were implanted in adult male Wistar rats for in vivo experimentation. Both crosslinked and uncrosslinked jellyfish collagen sponges showed a significant reduction in histopathology scores over the course of the study, whereas the bovine collagen sponge scores were not significantly reduced. Both jellyfish collagen sponges and the bovine sponge were tolerated well by the hosts, and a recovery was visible in all samples, suggesting that R. pulmo jellyfish‐derived collagen could offer compelling biocompatibility with wound healing applications. We also demonstrate that noncrosslinked samples could be safer with better resorption times than crosslinked samples. Journal Article Journal of Biomedical Materials Research Part B: Applied Biomaterials 106 4 1524 1533 1552-4973 jellyfish collagen, in vivo, regenerative medicine, collagen implantation, biomaterials 16 4 2018 2018-04-16 10.1002/jbm.b.33959 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2019-07-12T15:55:32.0977834 2018-05-03T11:10:51.2880650 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Jonathan Widdowson 1 Alex J. Picton 2 Valerie Vince 3 Chris J. Wright 4 Andrew Mearns-Spragg 5 Christopher Wright 0000-0003-2375-8159 6 0039891-15052018141954.pdf widdowson2017v2.pdf 2018-05-15T14:19:54.5400000 Output 708773 application/pdf Version of Record true 2018-05-15T00:00:00.0000000 true eng |
title |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices |
spellingShingle |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices Jonathan Widdowson Christopher Wright |
title_short |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices |
title_full |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices |
title_fullStr |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices |
title_full_unstemmed |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices |
title_sort |
In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices |
author_id_str_mv |
768d398f0d415f52c20b0b1deb93ce38 235e125ac3463e2ee7fc98604bf879ce |
author_id_fullname_str_mv |
768d398f0d415f52c20b0b1deb93ce38_***_Jonathan Widdowson 235e125ac3463e2ee7fc98604bf879ce_***_Christopher Wright |
author |
Jonathan Widdowson Christopher Wright |
author2 |
Jonathan Widdowson Alex J. Picton Valerie Vince Chris J. Wright Andrew Mearns-Spragg Christopher Wright |
format |
Journal article |
container_title |
Journal of Biomedical Materials Research Part B: Applied Biomaterials |
container_volume |
106 |
container_issue |
4 |
container_start_page |
1524 |
publishDate |
2018 |
institution |
Swansea University |
issn |
1552-4973 |
doi_str_mv |
10.1002/jbm.b.33959 |
college_str |
Faculty of Science and Engineering |
hierarchytype |
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facultyofscienceandengineering |
hierarchy_top_title |
Faculty of Science and Engineering |
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facultyofscienceandengineering |
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Faculty of Science and Engineering |
department_str |
School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering |
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description |
Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges. The scaffolds were prepared and examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and scanning electron microscopy (SEM). The samples were implanted in adult male Wistar rats for in vivo experimentation. Both crosslinked and uncrosslinked jellyfish collagen sponges showed a significant reduction in histopathology scores over the course of the study, whereas the bovine collagen sponge scores were not significantly reduced. Both jellyfish collagen sponges and the bovine sponge were tolerated well by the hosts, and a recovery was visible in all samples, suggesting that R. pulmo jellyfish‐derived collagen could offer compelling biocompatibility with wound healing applications. We also demonstrate that noncrosslinked samples could be safer with better resorption times than crosslinked samples. |
published_date |
2018-04-16T03:50:44Z |
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1763752484214407168 |
score |
11.035349 |