No Cover Image

Journal article 1470 views 183 downloads

Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture

IA Otto, R Levato, WR Webb, IM Khan, CC Breugem, J Malda, Ilyas Khan Orcid Logo

European Cells and Materials, Volume: 35, Pages: 132 - 150

Swansea University Author: Ilyas Khan Orcid Logo

  • 41044.pdf

    PDF | Version of Record

    This article is distributed in accordance with Creative Commons Attribution Licence.

    Download (2.72MB)

DOI (Published version): 10.22203/eCM.v035a10

Abstract

Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine str...

Full description

Published in: European Cells and Materials
Published: 2018
URI: https://cronfa.swan.ac.uk/Record/cronfa41044
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
College: Faculty of Medicine, Health and Life Sciences
Start Page: 132
End Page: 150