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Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture

IA Otto, R Levato, WR Webb, IM Khan, CC Breugem, J Malda, Ilyas Khan Orcid Logo

European Cells and Materials, Volume: 35, Pages: 132 - 150

Swansea University Author: Ilyas Khan Orcid Logo

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DOI (Published version): 10.22203/eCM.v035a10

Abstract

Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine str...

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Published in: European Cells and Materials
Published: 2018
URI: https://cronfa.swan.ac.uk/Record/cronfa41044
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spelling 2018-08-14T15:49:24.5948033 v2 41044 2018-07-17 Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture 2536d955ff70e7b77063a8efe9103161 0000-0002-3886-1987 Ilyas Khan Ilyas Khan true false 2018-07-17 BMS Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs. Journal Article European Cells and Materials 35 132 150 30 6 2018 2018-06-30 10.22203/eCM.v035a10 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2018-08-14T15:49:24.5948033 2018-07-17T15:21:42.9850781 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine IA Otto 1 R Levato 2 WR Webb 3 IM Khan 4 CC Breugem 5 J Malda 6 Ilyas Khan 0000-0002-3886-1987 7 0041044-14082018154621.pdf 41044.pdf 2018-08-14T15:46:21.0730000 Output 2857304 application/pdf Version of Record true 2018-08-14T00:00:00.0000000 This article is distributed in accordance with Creative Commons Attribution Licence. true eng
title Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
spellingShingle Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Ilyas Khan
title_short Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
title_full Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
title_fullStr Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
title_full_unstemmed Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
title_sort Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
author_id_str_mv 2536d955ff70e7b77063a8efe9103161
author_id_fullname_str_mv 2536d955ff70e7b77063a8efe9103161_***_Ilyas Khan
author Ilyas Khan
author2 IA Otto
R Levato
WR Webb
IM Khan
CC Breugem
J Malda
Ilyas Khan
format Journal article
container_title European Cells and Materials
container_volume 35
container_start_page 132
publishDate 2018
institution Swansea University
doi_str_mv 10.22203/eCM.v035a10
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.
published_date 2018-06-30T03:52:19Z
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