Journal article 1452 views 176 downloads
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
European Cells and Materials, Volume: 35, Pages: 132 - 150
Swansea University Author: Ilyas Khan
-
PDF | Version of Record
This article is distributed in accordance with Creative Commons Attribution Licence.
Download (2.72MB)
DOI (Published version): 10.22203/eCM.v035a10
Abstract
Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine str...
Published in: | European Cells and Materials |
---|---|
Published: |
2018
|
URI: | https://cronfa.swan.ac.uk/Record/cronfa41044 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
first_indexed |
2018-07-17T19:37:18Z |
---|---|
last_indexed |
2018-08-14T18:53:44Z |
id |
cronfa41044 |
recordtype |
SURis |
fullrecord |
<?xml version="1.0"?><rfc1807><datestamp>2018-08-14T15:49:24.5948033</datestamp><bib-version>v2</bib-version><id>41044</id><entry>2018-07-17</entry><title>Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture</title><swanseaauthors><author><sid>2536d955ff70e7b77063a8efe9103161</sid><ORCID>0000-0002-3886-1987</ORCID><firstname>Ilyas</firstname><surname>Khan</surname><name>Ilyas Khan</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2018-07-17</date><deptcode>BMS</deptcode><abstract>Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.</abstract><type>Journal Article</type><journal>European Cells and Materials</journal><volume>35</volume><paginationStart>132</paginationStart><paginationEnd>150</paginationEnd><publisher/><keywords/><publishedDay>30</publishedDay><publishedMonth>6</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-06-30</publishedDate><doi>10.22203/eCM.v035a10</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2018-08-14T15:49:24.5948033</lastEdited><Created>2018-07-17T15:21:42.9850781</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>IA</firstname><surname>Otto</surname><order>1</order></author><author><firstname>R</firstname><surname>Levato</surname><order>2</order></author><author><firstname>WR</firstname><surname>Webb</surname><order>3</order></author><author><firstname>IM</firstname><surname>Khan</surname><order>4</order></author><author><firstname>CC</firstname><surname>Breugem</surname><order>5</order></author><author><firstname>J</firstname><surname>Malda</surname><order>6</order></author><author><firstname>Ilyas</firstname><surname>Khan</surname><orcid>0000-0002-3886-1987</orcid><order>7</order></author></authors><documents><document><filename>0041044-14082018154621.pdf</filename><originalFilename>41044.pdf</originalFilename><uploaded>2018-08-14T15:46:21.0730000</uploaded><type>Output</type><contentLength>2857304</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-08-14T00:00:00.0000000</embargoDate><documentNotes>This article is distributed in accordance with Creative Commons Attribution Licence.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807> |
spelling |
2018-08-14T15:49:24.5948033 v2 41044 2018-07-17 Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture 2536d955ff70e7b77063a8efe9103161 0000-0002-3886-1987 Ilyas Khan Ilyas Khan true false 2018-07-17 BMS Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs. Journal Article European Cells and Materials 35 132 150 30 6 2018 2018-06-30 10.22203/eCM.v035a10 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2018-08-14T15:49:24.5948033 2018-07-17T15:21:42.9850781 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine IA Otto 1 R Levato 2 WR Webb 3 IM Khan 4 CC Breugem 5 J Malda 6 Ilyas Khan 0000-0002-3886-1987 7 0041044-14082018154621.pdf 41044.pdf 2018-08-14T15:46:21.0730000 Output 2857304 application/pdf Version of Record true 2018-08-14T00:00:00.0000000 This article is distributed in accordance with Creative Commons Attribution Licence. true eng |
title |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture |
spellingShingle |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture Ilyas Khan |
title_short |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture |
title_full |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture |
title_fullStr |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture |
title_full_unstemmed |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture |
title_sort |
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture |
author_id_str_mv |
2536d955ff70e7b77063a8efe9103161 |
author_id_fullname_str_mv |
2536d955ff70e7b77063a8efe9103161_***_Ilyas Khan |
author |
Ilyas Khan |
author2 |
IA Otto R Levato WR Webb IM Khan CC Breugem J Malda Ilyas Khan |
format |
Journal article |
container_title |
European Cells and Materials |
container_volume |
35 |
container_start_page |
132 |
publishDate |
2018 |
institution |
Swansea University |
doi_str_mv |
10.22203/eCM.v035a10 |
college_str |
Faculty of Medicine, Health and Life Sciences |
hierarchytype |
|
hierarchy_top_id |
facultyofmedicinehealthandlifesciences |
hierarchy_top_title |
Faculty of Medicine, Health and Life Sciences |
hierarchy_parent_id |
facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
document_store_str |
1 |
active_str |
0 |
description |
Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. Neocartilage formation was assessed through gene expression pro ling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs’ tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs. |
published_date |
2018-06-30T03:52:19Z |
_version_ |
1763752583124484096 |
score |
11.0267 |