No Cover Image

Journal article 253 views

Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors / Andrea Gazze; Richard Ademefun; Robert Steven Conlan; Sofia Rodrigues Teixeira; Steve Conlan; Salvatore Gazze

Journal of Interdisciplinary Nanomedicine, Volume: 3, Issue: 2, Pages: 82 - 88

Swansea University Authors: Steve, Conlan, Salvatore, Gazze

Full text not available from this repository: check for access using links below.

Check full text

DOI (Published version): 10.1002/jin2.40

Abstract

With current diagnostic methods detection of stage 1 or 2 ovarian cancer using CA125 is possible in only 75% of cases. The ability to detect CA125 at lower concentrations could significantly improve such early stage diagnosis. Here, the use of screen‐printed graphene biosensors as a label‐free detec...

Full description

Published in: Journal of Interdisciplinary Nanomedicine
ISSN: 2058-3273
Published: 2018
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa43526
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: With current diagnostic methods detection of stage 1 or 2 ovarian cancer using CA125 is possible in only 75% of cases. The ability to detect CA125 at lower concentrations could significantly improve such early stage diagnosis. Here, the use of screen‐printed graphene biosensors as a label‐free detection platform for CA125 was evaluated. The sensor was fabricated through deposition of a polyaniline layer via electropolymerisation on to a graphene screen‐printed electrode. The sensor surface was functionalised with anti‐CA125 antibody via covalent cross linking to polyaniline. The fabrication process was characterised through cyclic voltammetry and electrochemical impedance spectroscopy. The limit of detection achieved was 0.923 ng/μL across a dynamic range of 0.92 pg/μL–15.20 ng/μL and represents the most sensitive CA125 detection reported to date. With sensitivity limits at this level, it will now be possible to conduct clinical trials using serum samples collected from early stage ovarian cancer patients and at risk individuals.
Keywords: Graphene, immunosensor, ovarian cancer, CA125, screen‐printed electrode
Issue: 2
Start Page: 82
End Page: 88