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Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors

Andrea Gazze, Richard Ademefun, Robert Steven Conlan, Sofia Rodrigues Teixeira, Steve Conlan Orcid Logo, Salvatore Gazze

Journal of Interdisciplinary Nanomedicine, Volume: 3, Issue: 2, Pages: 82 - 88

Swansea University Authors: Steve Conlan Orcid Logo, Salvatore Gazze

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DOI (Published version): 10.1002/jin2.40

Abstract

With current diagnostic methods detection of stage 1 or 2 ovarian cancer using CA125 is possible in only 75% of cases. The ability to detect CA125 at lower concentrations could significantly improve such early stage diagnosis. Here, the use of screen‐printed graphene biosensors as a label‐free detec...

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Published in: Journal of Interdisciplinary Nanomedicine
ISSN: 2058-3273
Published: 2018
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa43526
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first_indexed 2018-08-20T13:53:38Z
last_indexed 2018-10-15T19:17:32Z
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spelling 2018-10-15T15:12:09.9327989 v2 43526 2018-08-20 Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors 0bb6bd247e32fb4249de62c0013b51cb 0000-0002-2562-3461 Steve Conlan Steve Conlan true false 586f1f49652b97c5c3ab99a45a1c58bf Salvatore Gazze Salvatore Gazze true false 2018-08-20 BMS With current diagnostic methods detection of stage 1 or 2 ovarian cancer using CA125 is possible in only 75% of cases. The ability to detect CA125 at lower concentrations could significantly improve such early stage diagnosis. Here, the use of screen‐printed graphene biosensors as a label‐free detection platform for CA125 was evaluated. The sensor was fabricated through deposition of a polyaniline layer via electropolymerisation on to a graphene screen‐printed electrode. The sensor surface was functionalised with anti‐CA125 antibody via covalent cross linking to polyaniline. The fabrication process was characterised through cyclic voltammetry and electrochemical impedance spectroscopy. The limit of detection achieved was 0.923 ng/μL across a dynamic range of 0.92 pg/μL–15.20 ng/μL and represents the most sensitive CA125 detection reported to date. With sensitivity limits at this level, it will now be possible to conduct clinical trials using serum samples collected from early stage ovarian cancer patients and at risk individuals. Journal Article Journal of Interdisciplinary Nanomedicine 3 2 82 88 2058-3273 Graphene, immunosensor, ovarian cancer, CA125, screen‐printed electrode 30 6 2018 2018-06-30 10.1002/jin2.40 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2018-10-15T15:12:09.9327989 2018-08-20T12:12:21.3054157 Andrea Gazze 1 Richard Ademefun 2 Robert Steven Conlan 3 Sofia Rodrigues Teixeira 4 Steve Conlan 0000-0002-2562-3461 5 Salvatore Gazze 6
title Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
spellingShingle Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
Steve Conlan
Salvatore Gazze
title_short Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
title_full Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
title_fullStr Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
title_full_unstemmed Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
title_sort Electrochemical impedence spectroscopy enabled CA125 detection; toward early ovarian cancer diagnosis using graphene biosensors
author_id_str_mv 0bb6bd247e32fb4249de62c0013b51cb
586f1f49652b97c5c3ab99a45a1c58bf
author_id_fullname_str_mv 0bb6bd247e32fb4249de62c0013b51cb_***_Steve Conlan
586f1f49652b97c5c3ab99a45a1c58bf_***_Salvatore Gazze
author Steve Conlan
Salvatore Gazze
author2 Andrea Gazze
Richard Ademefun
Robert Steven Conlan
Sofia Rodrigues Teixeira
Steve Conlan
Salvatore Gazze
format Journal article
container_title Journal of Interdisciplinary Nanomedicine
container_volume 3
container_issue 2
container_start_page 82
publishDate 2018
institution Swansea University
issn 2058-3273
doi_str_mv 10.1002/jin2.40
document_store_str 0
active_str 0
description With current diagnostic methods detection of stage 1 or 2 ovarian cancer using CA125 is possible in only 75% of cases. The ability to detect CA125 at lower concentrations could significantly improve such early stage diagnosis. Here, the use of screen‐printed graphene biosensors as a label‐free detection platform for CA125 was evaluated. The sensor was fabricated through deposition of a polyaniline layer via electropolymerisation on to a graphene screen‐printed electrode. The sensor surface was functionalised with anti‐CA125 antibody via covalent cross linking to polyaniline. The fabrication process was characterised through cyclic voltammetry and electrochemical impedance spectroscopy. The limit of detection achieved was 0.923 ng/μL across a dynamic range of 0.92 pg/μL–15.20 ng/μL and represents the most sensitive CA125 detection reported to date. With sensitivity limits at this level, it will now be possible to conduct clinical trials using serum samples collected from early stage ovarian cancer patients and at risk individuals.
published_date 2018-06-30T03:54:44Z
_version_ 1763752736196657152
score 11.036706

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