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RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

Mónica R. Romero, Andrés Pérez-Figueroa, Mónica Carrera, Willie J. Swanson, David Skibinski Orcid Logo, Angel P. Diz

Journal of Proteomics

Swansea University Author: David Skibinski Orcid Logo

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DOI (Published version): 10.1016/j.jprot.2018.08.020

Abstract

(a) RNA based approach to assess genomic differences between a Welsh and Spanish species of Mussell. Protein based analysis also carried out, both in gonad tissue. Not too sure why DNA based approaches not used? Clearly differences were observed. Lots of detailed analysis of differentially expressed...

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Published in: Journal of Proteomics
ISSN: 18743919
Published: 2019
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa44682
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Abstract: (a) RNA based approach to assess genomic differences between a Welsh and Spanish species of Mussell. Protein based analysis also carried out, both in gonad tissue. Not too sure why DNA based approaches not used? Clearly differences were observed. Lots of detailed analysis of differentially expressed genes. Not too sure about the significance of this data - this could be my lack of understanding. (b) Technically very competent transcriptomic and proteomic analysis of marine mussel sperm. However, the paper does not really get to grips with the underlying biological problem: that the two species studied are only partly reproductively isolated. With some hybridization being possible. This is discussed briefly in section 4.3.3, but the conclusions are very much in the form of hypothetical but undecided possibilities; as, indeed, are the conclusions generally.
College: Faculty of Medicine, Health and Life Sciences

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