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RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

Mónica R. Romero, Andrés Pérez-Figueroa, Mónica Carrera, Willie J. Swanson, David Skibinski Orcid Logo, Angel P. Diz

Journal of Proteomics

Swansea University Author: David Skibinski Orcid Logo

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DOI (Published version): 10.1016/j.jprot.2018.08.020

Abstract

(a) RNA based approach to assess genomic differences between a Welsh and Spanish species of Mussell. Protein based analysis also carried out, both in gonad tissue. Not too sure why DNA based approaches not used? Clearly differences were observed. Lots of detailed analysis of differentially expressed...

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Published in: Journal of Proteomics
ISSN: 18743919
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa44682
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first_indexed 2018-10-02T19:02:23Z
last_indexed 2023-01-11T14:21:23Z
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spelling 2022-09-27T16:35:30.1536984 v2 44682 2018-10-02 RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species 328d16903f98c2b03a1cc64a7530322a 0000-0003-4077-6236 David Skibinski David Skibinski true false 2018-10-02 SGMED (a) RNA based approach to assess genomic differences between a Welsh and Spanish species of Mussell. Protein based analysis also carried out, both in gonad tissue. Not too sure why DNA based approaches not used? Clearly differences were observed. Lots of detailed analysis of differentially expressed genes. Not too sure about the significance of this data - this could be my lack of understanding. (b) Technically very competent transcriptomic and proteomic analysis of marine mussel sperm. However, the paper does not really get to grips with the underlying biological problem: that the two species studied are only partly reproductively isolated. With some hybridization being possible. This is discussed briefly in section 4.3.3, but the conclusions are very much in the form of hypothetical but undecided possibilities; as, indeed, are the conclusions generally. Journal Article Journal of Proteomics 18743919 28 2 2019 2019-02-28 10.1016/j.jprot.2018.08.020 COLLEGE NANME Medical School - School COLLEGE CODE SGMED Swansea University 2022-09-27T16:35:30.1536984 2018-10-02T14:37:37.6299451 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Mónica R. Romero 1 Andrés Pérez-Figueroa 2 Mónica Carrera 3 Willie J. Swanson 4 David Skibinski 0000-0003-4077-6236 5 Angel P. Diz 6 0044682-25102018204342.pdf JPROT-D-18-00295R1Paper.pdf 2018-10-25T20:43:42.7270000 Output 1007438 application/pdf Accepted Manuscript true 2019-09-04T00:00:00.0000000 Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND). true eng
title RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
spellingShingle RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
David Skibinski
title_short RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_full RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_fullStr RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_full_unstemmed RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_sort RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
author_id_str_mv 328d16903f98c2b03a1cc64a7530322a
author_id_fullname_str_mv 328d16903f98c2b03a1cc64a7530322a_***_David Skibinski
author David Skibinski
author2 Mónica R. Romero
Andrés Pérez-Figueroa
Mónica Carrera
Willie J. Swanson
David Skibinski
Angel P. Diz
format Journal article
container_title Journal of Proteomics
publishDate 2019
institution Swansea University
issn 18743919
doi_str_mv 10.1016/j.jprot.2018.08.020
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description (a) RNA based approach to assess genomic differences between a Welsh and Spanish species of Mussell. Protein based analysis also carried out, both in gonad tissue. Not too sure why DNA based approaches not used? Clearly differences were observed. Lots of detailed analysis of differentially expressed genes. Not too sure about the significance of this data - this could be my lack of understanding. (b) Technically very competent transcriptomic and proteomic analysis of marine mussel sperm. However, the paper does not really get to grips with the underlying biological problem: that the two species studied are only partly reproductively isolated. With some hybridization being possible. This is discussed briefly in section 4.3.3, but the conclusions are very much in the form of hypothetical but undecided possibilities; as, indeed, are the conclusions generally.
published_date 2019-02-28T03:56:01Z
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score 11.012678

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