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Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone

Angel P Diz, Mónica R Romero, Juan Galindo, María Saura, David Skibinski Orcid Logo, Emilio Rolán-Alvarez

Current Zoology

Swansea University Author: David Skibinski Orcid Logo

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DOI (Published version): 10.1093/cz/zoab054

Abstract

Proteomic analysis was carried out on the Crab (upper-shore) and Wave (lower-shore) ecotypes ofLittorina saxatilis from a hybrid zone at Silleiro Cape, Spain. Proteome profiles of individual snailswere obtained. Protein expression in F1 hybrid snails bred in the laboratory and snails with intermedia...

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Published in: Current Zoology
ISSN: 1674-5507 2396-9814
Published: Oxford University Press (OUP) 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa57607
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Abstract: Proteomic analysis was carried out on the Crab (upper-shore) and Wave (lower-shore) ecotypes ofLittorina saxatilis from a hybrid zone at Silleiro Cape, Spain. Proteome profiles of individual snailswere obtained. Protein expression in F1 hybrid snails bred in the laboratory and snails with intermediate shell phenotypes collected from the mid-shore were compared with Crab and Wave ecotypes using analytical approaches used to study dominance. Multivariate analysis over many proteinspots showed that the F1 snails are distinct from both ecotypes but closer to the Wave ecotype. Theintermediate snails are highly variable, some closer to the Crab and others to the Wave ecotype.Considered on a protein by protein basis, some proteins are significantly closer in expression to theCrab and others to the Wave ecotype for both F1 and intermediate snails. Furthermore, a significantmajority of proteins were closer in expression to the Wave ecotype for the F1, consistent with themultivariate analysis. No such significant majority toward either the Crab or Wave ecotype wasobserved for the intermediate snails. The closer similarity of F1 and Wave ecotype expression patterns could be the result of similar selective pressures in the similar mid-shore and low-shore environments. For a significantly larger number of proteins, intermediate snails were closer in expressionto the ecotype having the lower expression, for both Crab and Wave ecotypes. This is somewhat unexpected as lower expression might be expected to be an indication of impairment of function andlower fitness. Proteomic analysis could be important for the identification of candidate proteins useful for gaining improved understanding of adaptation and barriers to gene flow in hybrid zones.
Keywords: F1 hybrids, gene expression, marine invertebrates, molecular phenotype, reproductive isolation, speciation
College: Swansea University Medical School