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Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone

Angel P Diz, Mónica R Romero, Juan Galindo, María Saura, David Skibinski Orcid Logo, Emilio Rolán-Alvarez

Current Zoology, Volume: 68, Issue: 3

Swansea University Author: David Skibinski Orcid Logo

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DOI (Published version): 10.1093/cz/zoab054

Abstract

Proteomic analysis was carried out on the Crab (upper-shore) and Wave (lower-shore) ecotypes ofLittorina saxatilis from a hybrid zone at Silleiro Cape, Spain. Proteome profiles of individual snailswere obtained. Protein expression in F1 hybrid snails bred in the laboratory and snails with intermedia...

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Published in: Current Zoology
ISSN: 1674-5507 2396-9814
Published: Oxford University Press (OUP) 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa57607
first_indexed 2021-09-06T15:03:14Z
last_indexed 2022-06-14T03:14:45Z
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spelling 2022-06-13T15:20:28.6856120 v2 57607 2021-08-12 Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone 328d16903f98c2b03a1cc64a7530322a 0000-0003-4077-6236 David Skibinski David Skibinski true false 2021-08-12 MEDS Proteomic analysis was carried out on the Crab (upper-shore) and Wave (lower-shore) ecotypes ofLittorina saxatilis from a hybrid zone at Silleiro Cape, Spain. Proteome profiles of individual snailswere obtained. Protein expression in F1 hybrid snails bred in the laboratory and snails with intermediate shell phenotypes collected from the mid-shore were compared with Crab and Wave ecotypes using analytical approaches used to study dominance. Multivariate analysis over many proteinspots showed that the F1 snails are distinct from both ecotypes but closer to the Wave ecotype. Theintermediate snails are highly variable, some closer to the Crab and others to the Wave ecotype.Considered on a protein by protein basis, some proteins are significantly closer in expression to theCrab and others to the Wave ecotype for both F1 and intermediate snails. Furthermore, a significantmajority of proteins were closer in expression to the Wave ecotype for the F1, consistent with themultivariate analysis. No such significant majority toward either the Crab or Wave ecotype wasobserved for the intermediate snails. The closer similarity of F1 and Wave ecotype expression patterns could be the result of similar selective pressures in the similar mid-shore and low-shore environments. For a significantly larger number of proteins, intermediate snails were closer in expressionto the ecotype having the lower expression, for both Crab and Wave ecotypes. This is somewhat unexpected as lower expression might be expected to be an indication of impairment of function andlower fitness. Proteomic analysis could be important for the identification of candidate proteins useful for gaining improved understanding of adaptation and barriers to gene flow in hybrid zones. Journal Article Current Zoology 68 3 Oxford University Press (OUP) 1674-5507 2396-9814 F1 hybrids, gene expression, marine invertebrates, molecular phenotype, reproductive isolation, speciation 10 8 2021 2021-08-10 10.1093/cz/zoab054 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2022-06-13T15:20:28.6856120 2021-08-12T21:16:25.8022963 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Angel P Diz 1 Mónica R Romero 2 Juan Galindo 3 María Saura 4 David Skibinski 0000-0003-4077-6236 5 Emilio Rolán-Alvarez 6 57607__20749__e848f23cc6764ff093a640797b391ca6.pdf 57607.pdf 2021-09-06T16:00:40.3811554 Output 590018 application/pdf Version of Record true The Author(s) (2021). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License true eng https://creativecommons.org/licenses/by-nc/4.0/
title Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
spellingShingle Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
David Skibinski
title_short Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
title_full Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
title_fullStr Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
title_full_unstemmed Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
title_sort Proteomic analysis of F1 hybrids and intermediate variants in a littorina saxatilis hybrid zone
author_id_str_mv 328d16903f98c2b03a1cc64a7530322a
author_id_fullname_str_mv 328d16903f98c2b03a1cc64a7530322a_***_David Skibinski
author David Skibinski
author2 Angel P Diz
Mónica R Romero
Juan Galindo
María Saura
David Skibinski
Emilio Rolán-Alvarez
format Journal article
container_title Current Zoology
container_volume 68
container_issue 3
publishDate 2021
institution Swansea University
issn 1674-5507
2396-9814
doi_str_mv 10.1093/cz/zoab054
publisher Oxford University Press (OUP)
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Proteomic analysis was carried out on the Crab (upper-shore) and Wave (lower-shore) ecotypes ofLittorina saxatilis from a hybrid zone at Silleiro Cape, Spain. Proteome profiles of individual snailswere obtained. Protein expression in F1 hybrid snails bred in the laboratory and snails with intermediate shell phenotypes collected from the mid-shore were compared with Crab and Wave ecotypes using analytical approaches used to study dominance. Multivariate analysis over many proteinspots showed that the F1 snails are distinct from both ecotypes but closer to the Wave ecotype. Theintermediate snails are highly variable, some closer to the Crab and others to the Wave ecotype.Considered on a protein by protein basis, some proteins are significantly closer in expression to theCrab and others to the Wave ecotype for both F1 and intermediate snails. Furthermore, a significantmajority of proteins were closer in expression to the Wave ecotype for the F1, consistent with themultivariate analysis. No such significant majority toward either the Crab or Wave ecotype wasobserved for the intermediate snails. The closer similarity of F1 and Wave ecotype expression patterns could be the result of similar selective pressures in the similar mid-shore and low-shore environments. For a significantly larger number of proteins, intermediate snails were closer in expressionto the ecotype having the lower expression, for both Crab and Wave ecotypes. This is somewhat unexpected as lower expression might be expected to be an indication of impairment of function andlower fitness. Proteomic analysis could be important for the identification of candidate proteins useful for gaining improved understanding of adaptation and barriers to gene flow in hybrid zones.
published_date 2021-08-10T20:16:46Z
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