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E-Thesis 506 views

The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications / Jonathan Widdowson

Swansea University Author: Jonathan Widdowson

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DOI (Published version): 10.23889/Suthesis.52446

Abstract

Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings h...

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Published: Swansea 2018
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa52446
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first_indexed 2019-10-15T14:31:53Z
last_indexed 2023-01-11T14:29:35Z
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spelling 2022-12-18T10:05:15.2464818 v2 52446 2019-10-15 The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications 768d398f0d415f52c20b0b1deb93ce38 Jonathan Widdowson Jonathan Widdowson true false 2019-10-15 FGSEN Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings have shown that collagen is irreversibly denatured into gelatin when electrospun using fluorinated alcohols. The work contained within this thesis describes the production of an industrially relevant, scalable process for the extraction of acid soluble collagen. The characterisation of this collagen is carried out, and the optimisation for use in bio-printing is assessed.This thesis introduces a novel, collagen derived biomaterial, termed Single Alpha-Chain Collagen, and the methods for its production are discussed within. Both Acid Soluble and Single Alpha-Chain Collagens are characterised using common techniques, including Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis and Fourier Transform InfraRed spectroscopy. The benefits of Single Alpha-Chain Collagen include a higher solubility profile than has been previously shown by other native collagens, permitting the production of novel prototype medical devices. Single Alpha-Chain Collagen extracts can be electrospun from benign solvents and physiological buffer systems, while preserving the nativity of the protein. Furthermore, it is shown within that Single Alpha-Chain Collagen extracts possess the ability to refibrillise into triple helical collagen when exposed to physiological buffers, a feature which is retained, even after electrospinning is carried out. The implications of these findings are compared to the literature as a solution to the current issues facing the electrospinning of collagen. E-Thesis Swansea Collagen, Tissue Engineering, Regenerative Medicine, Jellyfish, Biomaterials, Collagen Extraction 31 12 2018 2018-12-31 10.23889/Suthesis.52446 A selection of third party content is redacted or is partially redacted from this thesis. COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University Doctoral Ph.D KESS / KESS II 2022-12-18T10:05:15.2464818 2019-10-15T12:12:13.4381783 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Jonathan Widdowson 1 Under embargo Under embargo 2019-10-15T12:36:30.3500000 Output 12516693 application/pdf Redacted version - open access true 2024-06-30T00:00:00.0000000 true
title The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
spellingShingle The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
Jonathan Widdowson
title_short The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
title_full The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
title_fullStr The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
title_full_unstemmed The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
title_sort The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications
author_id_str_mv 768d398f0d415f52c20b0b1deb93ce38
author_id_fullname_str_mv 768d398f0d415f52c20b0b1deb93ce38_***_Jonathan Widdowson
author Jonathan Widdowson
author2 Jonathan Widdowson
format E-Thesis
publishDate 2018
institution Swansea University
doi_str_mv 10.23889/Suthesis.52446
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 0
active_str 0
description Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings have shown that collagen is irreversibly denatured into gelatin when electrospun using fluorinated alcohols. The work contained within this thesis describes the production of an industrially relevant, scalable process for the extraction of acid soluble collagen. The characterisation of this collagen is carried out, and the optimisation for use in bio-printing is assessed.This thesis introduces a novel, collagen derived biomaterial, termed Single Alpha-Chain Collagen, and the methods for its production are discussed within. Both Acid Soluble and Single Alpha-Chain Collagens are characterised using common techniques, including Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis and Fourier Transform InfraRed spectroscopy. The benefits of Single Alpha-Chain Collagen include a higher solubility profile than has been previously shown by other native collagens, permitting the production of novel prototype medical devices. Single Alpha-Chain Collagen extracts can be electrospun from benign solvents and physiological buffer systems, while preserving the nativity of the protein. Furthermore, it is shown within that Single Alpha-Chain Collagen extracts possess the ability to refibrillise into triple helical collagen when exposed to physiological buffers, a feature which is retained, even after electrospinning is carried out. The implications of these findings are compared to the literature as a solution to the current issues facing the electrospinning of collagen.
published_date 2018-12-31T04:04:49Z
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score 11.012678

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