E-Thesis 613 views 26 downloads
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications / Jonathan Widdowson
Swansea University Author: Jonathan Widdowson
-
PDF | Redacted version - open access
Download (11.84MB)
DOI (Published version): 10.23889/Suthesis.52446
Abstract
Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings h...
Published: |
Swansea
2018
|
---|---|
Institution: | Swansea University |
Degree level: | Doctoral |
Degree name: | Ph.D |
URI: | https://cronfa.swan.ac.uk/Record/cronfa52446 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
first_indexed |
2019-10-15T14:31:53Z |
---|---|
last_indexed |
2023-01-11T14:29:35Z |
id |
cronfa52446 |
recordtype |
RisThesis |
fullrecord |
<?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>52446</id><entry>2019-10-15</entry><title>The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications</title><swanseaauthors><author><sid>768d398f0d415f52c20b0b1deb93ce38</sid><firstname>Jonathan</firstname><surname>Widdowson</surname><name>Jonathan Widdowson</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2019-10-15</date><abstract>Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings have shown that collagen is irreversibly denatured into gelatin when electrospun using fluorinated alcohols. The work contained within this thesis describes the production of an industrially relevant, scalable process for the extraction of acid soluble collagen. The characterisation of this collagen is carried out, and the optimisation for use in bio-printing is assessed.This thesis introduces a novel, collagen derived biomaterial, termed Single Alpha-Chain Collagen, and the methods for its production are discussed within. Both Acid Soluble and Single Alpha-Chain Collagens are characterised using common techniques, including Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis and Fourier Transform InfraRed spectroscopy. The benefits of Single Alpha-Chain Collagen include a higher solubility profile than has been previously shown by other native collagens, permitting the production of novel prototype medical devices. Single Alpha-Chain Collagen extracts can be electrospun from benign solvents and physiological buffer systems, while preserving the nativity of the protein. Furthermore, it is shown within that Single Alpha-Chain Collagen extracts possess the ability to refibrillise into triple helical collagen when exposed to physiological buffers, a feature which is retained, even after electrospinning is carried out. The implications of these findings are compared to the literature as a solution to the current issues facing the electrospinning of collagen.</abstract><type>E-Thesis</type><journal/><volume/><journalNumber/><paginationStart/><paginationEnd/><publisher/><placeOfPublication>Swansea</placeOfPublication><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic/><keywords>Collagen, Tissue Engineering, Regenerative Medicine, Jellyfish, Biomaterials, Collagen Extraction</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-12-31</publishedDate><doi>10.23889/Suthesis.52446</doi><url/><notes>A selection of third party content is redacted or is partially redacted from this thesis.</notes><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><degreelevel>Doctoral</degreelevel><degreename>Ph.D</degreename><degreesponsorsfunders>KESS / KESS II</degreesponsorsfunders><apcterm/><funders/><projectreference/><lastEdited>2024-07-11T15:37:00.6257758</lastEdited><Created>2019-10-15T12:12:13.4381783</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Uncategorised</level></path><authors><author><firstname>Jonathan</firstname><surname>Widdowson</surname><order>1</order></author></authors><documents><document><filename>0052446-15102019123630.pdf</filename><originalFilename>Widdowson_Jonathan_P_PhD_Thesis_Redacted_Embargoed30.06.2024.pdf</originalFilename><uploaded>2019-10-15T12:36:30.3500000</uploaded><type>Output</type><contentLength>12516693</contentLength><contentType>application/pdf</contentType><version>Redacted version - open access</version><cronfaStatus>true</cronfaStatus><embargoDate>2024-06-30T00:00:00.0000000</embargoDate><copyrightCorrect>true</copyrightCorrect></document></documents><OutputDurs/></rfc1807> |
spelling |
v2 52446 2019-10-15 The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications 768d398f0d415f52c20b0b1deb93ce38 Jonathan Widdowson Jonathan Widdowson true false 2019-10-15 Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings have shown that collagen is irreversibly denatured into gelatin when electrospun using fluorinated alcohols. The work contained within this thesis describes the production of an industrially relevant, scalable process for the extraction of acid soluble collagen. The characterisation of this collagen is carried out, and the optimisation for use in bio-printing is assessed.This thesis introduces a novel, collagen derived biomaterial, termed Single Alpha-Chain Collagen, and the methods for its production are discussed within. Both Acid Soluble and Single Alpha-Chain Collagens are characterised using common techniques, including Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis and Fourier Transform InfraRed spectroscopy. The benefits of Single Alpha-Chain Collagen include a higher solubility profile than has been previously shown by other native collagens, permitting the production of novel prototype medical devices. Single Alpha-Chain Collagen extracts can be electrospun from benign solvents and physiological buffer systems, while preserving the nativity of the protein. Furthermore, it is shown within that Single Alpha-Chain Collagen extracts possess the ability to refibrillise into triple helical collagen when exposed to physiological buffers, a feature which is retained, even after electrospinning is carried out. The implications of these findings are compared to the literature as a solution to the current issues facing the electrospinning of collagen. E-Thesis Swansea Collagen, Tissue Engineering, Regenerative Medicine, Jellyfish, Biomaterials, Collagen Extraction 31 12 2018 2018-12-31 10.23889/Suthesis.52446 A selection of third party content is redacted or is partially redacted from this thesis. COLLEGE NANME COLLEGE CODE Swansea University Doctoral Ph.D KESS / KESS II 2024-07-11T15:37:00.6257758 2019-10-15T12:12:13.4381783 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Jonathan Widdowson 1 0052446-15102019123630.pdf Widdowson_Jonathan_P_PhD_Thesis_Redacted_Embargoed30.06.2024.pdf 2019-10-15T12:36:30.3500000 Output 12516693 application/pdf Redacted version - open access true 2024-06-30T00:00:00.0000000 true |
title |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications |
spellingShingle |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications Jonathan Widdowson |
title_short |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications |
title_full |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications |
title_fullStr |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications |
title_full_unstemmed |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications |
title_sort |
The Extraction, Characterisation and Application of Novel Collagen-Based Bio-Materials Derived from Rhizostomas pulmo For Tissue Engineering Applications |
author_id_str_mv |
768d398f0d415f52c20b0b1deb93ce38 |
author_id_fullname_str_mv |
768d398f0d415f52c20b0b1deb93ce38_***_Jonathan Widdowson |
author |
Jonathan Widdowson |
author2 |
Jonathan Widdowson |
format |
E-Thesis |
publishDate |
2018 |
institution |
Swansea University |
doi_str_mv |
10.23889/Suthesis.52446 |
college_str |
Faculty of Science and Engineering |
hierarchytype |
|
hierarchy_top_id |
facultyofscienceandengineering |
hierarchy_top_title |
Faculty of Science and Engineering |
hierarchy_parent_id |
facultyofscienceandengineering |
hierarchy_parent_title |
Faculty of Science and Engineering |
department_str |
School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised |
document_store_str |
1 |
active_str |
0 |
description |
Collagen is the most abundant protein in animals, and as such provides the optimal polymer for use in tissue engineering and regenerative medicine applications. The electrospinning of collagen presents a simple, scalable method for the production of biocompatible scaffolds, however recent findings have shown that collagen is irreversibly denatured into gelatin when electrospun using fluorinated alcohols. The work contained within this thesis describes the production of an industrially relevant, scalable process for the extraction of acid soluble collagen. The characterisation of this collagen is carried out, and the optimisation for use in bio-printing is assessed.This thesis introduces a novel, collagen derived biomaterial, termed Single Alpha-Chain Collagen, and the methods for its production are discussed within. Both Acid Soluble and Single Alpha-Chain Collagens are characterised using common techniques, including Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis and Fourier Transform InfraRed spectroscopy. The benefits of Single Alpha-Chain Collagen include a higher solubility profile than has been previously shown by other native collagens, permitting the production of novel prototype medical devices. Single Alpha-Chain Collagen extracts can be electrospun from benign solvents and physiological buffer systems, while preserving the nativity of the protein. Furthermore, it is shown within that Single Alpha-Chain Collagen extracts possess the ability to refibrillise into triple helical collagen when exposed to physiological buffers, a feature which is retained, even after electrospinning is carried out. The implications of these findings are compared to the literature as a solution to the current issues facing the electrospinning of collagen. |
published_date |
2018-12-31T15:36:59Z |
_version_ |
1804293761618935808 |
score |
11.029921 |