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Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model / Daniel Rees, Luke Roberts, Carla Carisi, Alwena Morgan, Rowan Brown, Jeffrey Davies

Current Protocols in Neuroscience, Volume: 94, Issue: 1

Swansea University Authors: Daniel Rees, Luke Roberts, Carla Carisi, Alwena Morgan, Rowan Brown, Jeffrey Davies

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DOI (Published version): 10.1002/cpns.105

Abstract

Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identif...

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Published in: Current Protocols in Neuroscience
ISSN: 1934-8584 1934-8576
Published: Wiley 2020
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa55596
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Abstract: Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high‐content imaging (HCI), and the subsequent open‐source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone‐based in vitro Parkinson's disease model.
Keywords: fragmentation, image analysis, mitochondria, Parkinson's disease
College: Swansea University Medical School
Issue: 1