Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model

Rees, Daniel; Roberts, Luke; Carisi, Carla; Morgan, Alwena; Brown, Rowan; Davies, Jeffrey

doi:10.1002/cpns.105

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Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model

Daniel Rees

, Luke Roberts, Carla Carisi, Alwena Morgan

, Rowan Brown

, Jeffrey Davies

Current Protocols in Neuroscience, Volume: 94, Issue: 1

Swansea University Authors: Daniel Rees , Luke Roberts, Carla Carisi, Alwena Morgan , Rowan Brown , Jeffrey Davies

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DOI (Published version): 10.1002/cpns.105

Abstract

Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identif...

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Published in:	Current Protocols in Neuroscience
ISSN:	1934-8584 1934-8576
Published:	Wiley 2020
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URI:	https://cronfa.swan.ac.uk/Record/cronfa55596
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first_indexed	2020-11-05T14:31:00Z
last_indexed	2021-07-23T03:13:17Z
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Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. 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spelling	2021-07-22T14:22:35.9833478 v2 55596 2020-11-05 Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model daa6762111f9ebf62b9c2ec655512783 0000-0003-0372-6096 Daniel Rees Daniel Rees true false 22576ee8492628137b76a5b8cb68a384 Luke Roberts Luke Roberts true false b9eb37d84630e887855f17bdf94b6708 Carla Carisi Carla Carisi true false 9ea39c3d0935c897cb9fcd3ba550af71 0000-0002-3441-5357 Alwena Morgan Alwena Morgan true false d7db8d42c476dfa69c15ce06d29bd863 0000-0003-3628-2524 Rowan Brown Rowan Brown true false 2cb3d1d96a7870a84d2f758e865172e6 0000-0002-4234-0033 Jeffrey Davies Jeffrey Davies true false 2020-11-05 BBU Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high‐content imaging (HCI), and the subsequent open‐source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone‐based in vitro Parkinson's disease model. Journal Article Current Protocols in Neuroscience 94 1 Wiley 1934-8584 1934-8576 fragmentation, image analysis, mitochondria, Parkinson's disease 1 12 2020 2020-12-01 10.1002/cpns.105 COLLEGE NANME Business COLLEGE CODE BBU Swansea University SU Library paid the OA fee (TA Institutional Deal) Wiley TA; This work was funded by grants from the Medical Research Council (Grant no. G0902250), Parkinson's UK, St David's Medical Foundation, Coleg Cymraeg Cenedlaethol PhD Studentship, and a BRACE PhD Studentship. MRC G0902250 2021-07-22T14:22:35.9833478 2020-11-05T09:27:20.7382315 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Daniel Rees 0000-0003-0372-6096 1 Luke Roberts 2 Carla Carisi 3 Alwena Morgan 0000-0002-3441-5357 4 Rowan Brown 0000-0003-3628-2524 5 Jeffrey Davies 0000-0002-4234-0033 6 55596__18598__81c769049fa44b15a5b9c3a7acdc5f7e.pdf 55596.VOR.pdf 2020-11-05T14:37:57.4355774 Output 7348615 application/pdf Version of Record true This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. true eng https://creativecommons.org/licenses/by/4.0/
title	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
spellingShingle	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model Daniel Rees Luke Roberts Carla Carisi Alwena Morgan Rowan Brown Jeffrey Davies
title_short	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_full	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_fullStr	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_full_unstemmed	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_sort	Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
author_id_str_mv	daa6762111f9ebf62b9c2ec655512783 22576ee8492628137b76a5b8cb68a384 b9eb37d84630e887855f17bdf94b6708 9ea39c3d0935c897cb9fcd3ba550af71 d7db8d42c476dfa69c15ce06d29bd863 2cb3d1d96a7870a84d2f758e865172e6
author_id_fullname_str_mv	daa6762111f9ebf62b9c2ec655512783_*_Daniel Rees 22576ee8492628137b76a5b8cb68a384__Luke Roberts b9eb37d84630e887855f17bdf94b6708__Carla Carisi 9ea39c3d0935c897cb9fcd3ba550af71__Alwena Morgan d7db8d42c476dfa69c15ce06d29bd863__Rowan Brown 2cb3d1d96a7870a84d2f758e865172e6_*_Jeffrey Davies
author	Daniel Rees Luke Roberts Carla Carisi Alwena Morgan Rowan Brown Jeffrey Davies
author2	Daniel Rees Luke Roberts Carla Carisi Alwena Morgan Rowan Brown Jeffrey Davies
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container_title	Current Protocols in Neuroscience
container_volume	94
container_issue	1
publishDate	2020
institution	Swansea University
issn	1934-8584 1934-8576
doi_str_mv	10.1002/cpns.105
publisher	Wiley
college_str	Faculty of Medicine, Health and Life Sciences
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department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description	Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high‐content imaging (HCI), and the subsequent open‐source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone‐based in vitro Parkinson's disease model.
published_date	2020-12-01T04:09:55Z
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score	11.012678

Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model

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