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Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model / Daniel Rees, Luke Roberts, Carla Carisi, Alwena Morgan, Rowan Brown, Jeffrey Davies

Current Protocols in Neuroscience, Volume: 94, Issue: 1

Swansea University Authors: Daniel Rees, Luke Roberts, Carla Carisi, Alwena Morgan, Rowan Brown, Jeffrey Davies

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DOI (Published version): 10.1002/cpns.105

Abstract

Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identif...

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Published in: Current Protocols in Neuroscience
ISSN: 1934-8584 1934-8576
Published: Wiley 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa55596
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spelling 2021-07-22T14:22:35.9833478 v2 55596 2020-11-05 Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model daa6762111f9ebf62b9c2ec655512783 0000-0003-0372-6096 Daniel Rees Daniel Rees true false 22576ee8492628137b76a5b8cb68a384 Luke Roberts Luke Roberts true false b9eb37d84630e887855f17bdf94b6708 Carla Carisi Carla Carisi true false 9ea39c3d0935c897cb9fcd3ba550af71 0000-0002-3441-5357 Alwena Morgan Alwena Morgan true false d7db8d42c476dfa69c15ce06d29bd863 0000-0003-3628-2524 Rowan Brown Rowan Brown true false 2cb3d1d96a7870a84d2f758e865172e6 0000-0002-4234-0033 Jeffrey Davies Jeffrey Davies true false 2020-11-05 BBU Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high‐content imaging (HCI), and the subsequent open‐source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone‐based in vitro Parkinson's disease model. Journal Article Current Protocols in Neuroscience 94 1 Wiley 1934-8584 1934-8576 fragmentation, image analysis, mitochondria, Parkinson&apos;s disease 1 12 2020 2020-12-01 10.1002/cpns.105 COLLEGE NANME Business COLLEGE CODE BBU Swansea University SU Library paid the OA fee (TA Institutional Deal) Wiley TA; This work was funded by grants from the Medical Research Council (Grant no. G0902250), Parkinson's UK, St David's Medical Foundation, Coleg Cymraeg Cenedlaethol PhD Studentship, and a BRACE PhD Studentship. MRC G0902250 2021-07-22T14:22:35.9833478 2020-11-05T09:27:20.7382315 Swansea University Medical School Medicine Daniel Rees 0000-0003-0372-6096 1 Luke Roberts 2 Carla Carisi 3 Alwena Morgan 0000-0002-3441-5357 4 Rowan Brown 0000-0003-3628-2524 5 Jeffrey Davies 0000-0002-4234-0033 6 55596__18598__81c769049fa44b15a5b9c3a7acdc5f7e.pdf 55596.VOR.pdf 2020-11-05T14:37:57.4355774 Output 7348615 application/pdf Version of Record true This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. true eng
title Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
spellingShingle Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
Daniel, Rees
Luke, Roberts
Carla, Carisi
Alwena, Morgan
Rowan, Brown
Jeffrey, Davies
title_short Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_full Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_fullStr Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_full_unstemmed Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
title_sort Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
author_id_str_mv daa6762111f9ebf62b9c2ec655512783
22576ee8492628137b76a5b8cb68a384
b9eb37d84630e887855f17bdf94b6708
9ea39c3d0935c897cb9fcd3ba550af71
d7db8d42c476dfa69c15ce06d29bd863
2cb3d1d96a7870a84d2f758e865172e6
author_id_fullname_str_mv daa6762111f9ebf62b9c2ec655512783_***_Daniel, Rees
22576ee8492628137b76a5b8cb68a384_***_Luke, Roberts
b9eb37d84630e887855f17bdf94b6708_***_Carla, Carisi
9ea39c3d0935c897cb9fcd3ba550af71_***_Alwena, Morgan
d7db8d42c476dfa69c15ce06d29bd863_***_Rowan, Brown
2cb3d1d96a7870a84d2f758e865172e6_***_Jeffrey, Davies
author Daniel, Rees
Luke, Roberts
Carla, Carisi
Alwena, Morgan
Rowan, Brown
Jeffrey, Davies
author2 Daniel Rees
Luke Roberts
Carla Carisi
Alwena Morgan
Rowan Brown
Jeffrey Davies
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container_volume 94
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publishDate 2020
institution Swansea University
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1934-8576
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description Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high‐content imaging (HCI), and the subsequent open‐source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone‐based in vitro Parkinson's disease model.
published_date 2020-12-01T04:21:00Z
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