No Cover Image

Journal article 25 views 3 downloads

ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it

Venkat Kanamarlapudi Orcid Logo, Salam Tamaddon Jahromi, Kate Murphy Orcid Logo

PLOS ONE, Volume: 17, Issue: 2, Start page: e0263845

Swansea University Authors: Venkat Kanamarlapudi Orcid Logo, Salam Tamaddon Jahromi

  • 60111.pdf

    PDF | Version of Record

    © 2022 Kanamarlapudi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License

    Download (2.1MB)

Abstract

ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a w...

Full description

Published in: PLOS ONE
ISSN: 1932-6203
Published: Public Library of Science (PLoS) 2022
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa60111
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a wide range of tissues has not been studied so far. In this report, we analysed the expression of ARF6 mRNA in cancers and corresponding healthy controls from 17 different tissues by real-time qualitative polymerase chain reaction (RT-qPCR). We further evaluated ARF6 protein expression in oesophageal adenocarcinoma (EAC) tissue microarray cores by immunohistochemistry. The ARF6 gene expression levels are highly variable between healthy and cancer tissues. Our findings suggest that the ARF6 gene expression is up-regulated highest in oesophageal cancer. In EAC TMAs, ARF6 protein expression increase correlated with EAC progression. This is the first study to investigate ARF6 gene expression in a wide array of cancer tissues and demonstrate that ARF6 expression, at both mRNA and protein levels, is significantly upregulated in higher grades of EAC, which may be useful in targeting ARF6 for cancer diagnostic and therapeutic purposes.
College: Swansea University Medical School
Funders: VK: BBSRC UK (BB/F017596/1, BB/C515455/2 and BB/S019588/1) and MRC UK (G0401232)
Issue: 2
Start Page: e0263845