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ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
PLOS ONE, Volume: 17, Issue: 2, Start page: e0263845
Swansea University Authors:
Venkat Kanamarlapudi , Salam Tamaddon Jahromi
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DOI (Published version): 10.1371/journal.pone.0263845
Abstract
ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a w...
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v2 60111 2022-05-30 ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it 63741801137148abfa4c00cd547dcdfa 0000-0002-8739-1483 Venkat Kanamarlapudi Venkat Kanamarlapudi true false 1a74754e3fabec6379651cbd842c96bf Salam Tamaddon Jahromi Salam Tamaddon Jahromi true false 2022-05-30 MEDS ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a wide range of tissues has not been studied so far. In this report, we analysed the expression of ARF6 mRNA in cancers and corresponding healthy controls from 17 different tissues by real-time qualitative polymerase chain reaction (RT-qPCR). We further evaluated ARF6 protein expression in oesophageal adenocarcinoma (EAC) tissue microarray cores by immunohistochemistry. The ARF6 gene expression levels are highly variable between healthy and cancer tissues. Our findings suggest that the ARF6 gene expression is up-regulated highest in oesophageal cancer. In EAC TMAs, ARF6 protein expression increase correlated with EAC progression. This is the first study to investigate ARF6 gene expression in a wide array of cancer tissues and demonstrate that ARF6 expression, at both mRNA and protein levels, is significantly upregulated in higher grades of EAC, which may be useful in targeting ARF6 for cancer diagnostic and therapeutic purposes. Journal Article PLOS ONE 17 2 e0263845 Public Library of Science (PLoS) 1932-6203 10 2 2022 2022-02-10 10.1371/journal.pone.0263845 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University VK: BBSRC UK (BB/F017596/1, BB/C515455/2 and BB/S019588/1) and MRC UK (G0401232) 2024-07-15T11:55:03.6761925 2022-05-30T18:01:55.5116818 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Venkat Kanamarlapudi 0000-0002-8739-1483 1 Salam Tamaddon Jahromi 2 Kate Murphy 0000-0003-2870-3018 3 60111__24304__2b541405918c450582bcca203962e5ab.pdf 60111.pdf 2022-06-14T11:06:41.7227799 Output 2203943 application/pdf Version of Record true © 2022 Kanamarlapudi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License true eng http://creativecommons.org/licenses/by/4.0/ |
title |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it |
spellingShingle |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it Venkat Kanamarlapudi Salam Tamaddon Jahromi |
title_short |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it |
title_full |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it |
title_fullStr |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it |
title_full_unstemmed |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it |
title_sort |
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it |
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63741801137148abfa4c00cd547dcdfa 1a74754e3fabec6379651cbd842c96bf |
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63741801137148abfa4c00cd547dcdfa_***_Venkat Kanamarlapudi 1a74754e3fabec6379651cbd842c96bf_***_Salam Tamaddon Jahromi |
author |
Venkat Kanamarlapudi Salam Tamaddon Jahromi |
author2 |
Venkat Kanamarlapudi Salam Tamaddon Jahromi Kate Murphy |
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PLOS ONE |
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17 |
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e0263845 |
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2022 |
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Swansea University |
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1932-6203 |
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10.1371/journal.pone.0263845 |
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Public Library of Science (PLoS) |
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Faculty of Medicine, Health and Life Sciences |
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description |
ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a wide range of tissues has not been studied so far. In this report, we analysed the expression of ARF6 mRNA in cancers and corresponding healthy controls from 17 different tissues by real-time qualitative polymerase chain reaction (RT-qPCR). We further evaluated ARF6 protein expression in oesophageal adenocarcinoma (EAC) tissue microarray cores by immunohistochemistry. The ARF6 gene expression levels are highly variable between healthy and cancer tissues. Our findings suggest that the ARF6 gene expression is up-regulated highest in oesophageal cancer. In EAC TMAs, ARF6 protein expression increase correlated with EAC progression. This is the first study to investigate ARF6 gene expression in a wide array of cancer tissues and demonstrate that ARF6 expression, at both mRNA and protein levels, is significantly upregulated in higher grades of EAC, which may be useful in targeting ARF6 for cancer diagnostic and therapeutic purposes. |
published_date |
2022-02-10T11:55:02Z |
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11.021648 |