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Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity

Zara Ahmed, Lydia Powell Orcid Logo, Navid Matin, Andrew Mearns-Spragg Orcid Logo, Catherine A. Thornton, Ilyas M. Khan, Lewis Francis Orcid Logo

Marine Drugs, Volume: 19, Issue: 8, Start page: 405

Swansea University Authors: Lydia Powell Orcid Logo, Lewis Francis Orcid Logo

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DOI (Published version): 10.3390/md19080405

Abstract

Osteoarthritis (OA) is a multifactorial disease leading to degeneration of articular cartilage, causing morbidity in approximately 8.5 million of the UK population. As the dense extracellular matrix of articular cartilage is primarily composed of collagen, cartilage repair strategies have exploited...

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Published in: Marine Drugs
ISSN: 1660-3397
Published: MDPI AG 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa61611
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As the dense extracellular matrix of articular cartilage is primarily composed of collagen, cartilage repair strategies have exploited the biocompatibility and mechanical strength of bovine and porcine collagen to produce robust scaffolds for procedures such as matrix-induced chondrocyte implantation (MACI). However, mammalian sourced collagens pose safety risks such as bovine spongiform encephalopathy, transmissible spongiform encephalopathy and possible transmission of viral vectors. This study characterised a non-mammalian jellyfish (Rhizostoma pulmo) collagen as an alternative, safer source in scaffold production for clinical use. Jellyfish collagen demonstrated comparable scaffold structural properties and stability when compared to mammalian collagen. Jellyfish collagen also displayed comparable immunogenic responses (platelet and leukocyte activation/cell death) and cytokine release profile in comparison to mammalian collagen in vitro. Further histological analysis of jellyfish collagen revealed bovine chondroprogenitor cell invasion and proliferation in the scaffold structures, where the scaffold supported enhanced chondrogenesis in the presence of TGFβ1. This study highlights the potential of jellyfish collagen as a safe and biocompatible biomaterial for both OA repair and further regenerative medicine applications.</abstract><type>Journal Article</type><journal>Marine Drugs</journal><volume>19</volume><journalNumber>8</journalNumber><paginationStart>405</paginationStart><paginationEnd/><publisher>MDPI AG</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>1660-3397</issnElectronic><keywords>osteoarthritis; articular cartilage; jellyfish collagen; MACI</keywords><publishedDay>22</publishedDay><publishedMonth>7</publishedMonth><publishedYear>2021</publishedYear><publishedDate>2021-07-22</publishedDate><doi>10.3390/md19080405</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>This study was a collaboration between Swansea University and Jellagen Ltd through the Knowledge Economy Skills Scholarship (KESS) part funded by the European Regional Development Fund through the Welsh Government (KESS 2 c80815). 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spelling v2 61611 2022-10-20 Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity 0e7e702952672bcbfdfd4974199202fb 0000-0002-8641-0160 Lydia Powell Lydia Powell true false 10f61f9c1248951c1a33f6a89498f37d 0000-0002-7803-7714 Lewis Francis Lewis Francis true false 2022-10-20 BMS Osteoarthritis (OA) is a multifactorial disease leading to degeneration of articular cartilage, causing morbidity in approximately 8.5 million of the UK population. As the dense extracellular matrix of articular cartilage is primarily composed of collagen, cartilage repair strategies have exploited the biocompatibility and mechanical strength of bovine and porcine collagen to produce robust scaffolds for procedures such as matrix-induced chondrocyte implantation (MACI). However, mammalian sourced collagens pose safety risks such as bovine spongiform encephalopathy, transmissible spongiform encephalopathy and possible transmission of viral vectors. This study characterised a non-mammalian jellyfish (Rhizostoma pulmo) collagen as an alternative, safer source in scaffold production for clinical use. Jellyfish collagen demonstrated comparable scaffold structural properties and stability when compared to mammalian collagen. Jellyfish collagen also displayed comparable immunogenic responses (platelet and leukocyte activation/cell death) and cytokine release profile in comparison to mammalian collagen in vitro. Further histological analysis of jellyfish collagen revealed bovine chondroprogenitor cell invasion and proliferation in the scaffold structures, where the scaffold supported enhanced chondrogenesis in the presence of TGFβ1. This study highlights the potential of jellyfish collagen as a safe and biocompatible biomaterial for both OA repair and further regenerative medicine applications. Journal Article Marine Drugs 19 8 405 MDPI AG 1660-3397 osteoarthritis; articular cartilage; jellyfish collagen; MACI 22 7 2021 2021-07-22 10.3390/md19080405 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University This study was a collaboration between Swansea University and Jellagen Ltd through the Knowledge Economy Skills Scholarship (KESS) part funded by the European Regional Development Fund through the Welsh Government (KESS 2 c80815). This work was also supported by SMARTExpertise 2014-2020 West Wales and the Valleys, European Regional Development Fund, under Grant 2017/COL/004. 2024-02-01T15:54:50.8537309 2022-10-20T14:20:09.1826185 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Zara Ahmed 1 Lydia Powell 0000-0002-8641-0160 2 Navid Matin 3 Andrew Mearns-Spragg 0000-0003-1105-2677 4 Catherine A. Thornton 5 Ilyas M. Khan 6 Lewis Francis 0000-0002-7803-7714 7 61611__25714__0d401a839e684d5aa326aa30f7c16409.pdf 61611.pdf 2022-11-09T13:03:54.7556393 Output 3245424 application/pdf Version of Record true © 2021 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license true eng https://creativecommons.org/licenses/by/4.0/
title Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
spellingShingle Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
Lydia Powell
Lewis Francis
title_short Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
title_full Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
title_fullStr Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
title_full_unstemmed Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
title_sort Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity
author_id_str_mv 0e7e702952672bcbfdfd4974199202fb
10f61f9c1248951c1a33f6a89498f37d
author_id_fullname_str_mv 0e7e702952672bcbfdfd4974199202fb_***_Lydia Powell
10f61f9c1248951c1a33f6a89498f37d_***_Lewis Francis
author Lydia Powell
Lewis Francis
author2 Zara Ahmed
Lydia Powell
Navid Matin
Andrew Mearns-Spragg
Catherine A. Thornton
Ilyas M. Khan
Lewis Francis
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container_title Marine Drugs
container_volume 19
container_issue 8
container_start_page 405
publishDate 2021
institution Swansea University
issn 1660-3397
doi_str_mv 10.3390/md19080405
publisher MDPI AG
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Osteoarthritis (OA) is a multifactorial disease leading to degeneration of articular cartilage, causing morbidity in approximately 8.5 million of the UK population. As the dense extracellular matrix of articular cartilage is primarily composed of collagen, cartilage repair strategies have exploited the biocompatibility and mechanical strength of bovine and porcine collagen to produce robust scaffolds for procedures such as matrix-induced chondrocyte implantation (MACI). However, mammalian sourced collagens pose safety risks such as bovine spongiform encephalopathy, transmissible spongiform encephalopathy and possible transmission of viral vectors. This study characterised a non-mammalian jellyfish (Rhizostoma pulmo) collagen as an alternative, safer source in scaffold production for clinical use. Jellyfish collagen demonstrated comparable scaffold structural properties and stability when compared to mammalian collagen. Jellyfish collagen also displayed comparable immunogenic responses (platelet and leukocyte activation/cell death) and cytokine release profile in comparison to mammalian collagen in vitro. Further histological analysis of jellyfish collagen revealed bovine chondroprogenitor cell invasion and proliferation in the scaffold structures, where the scaffold supported enhanced chondrogenesis in the presence of TGFβ1. This study highlights the potential of jellyfish collagen as a safe and biocompatible biomaterial for both OA repair and further regenerative medicine applications.
published_date 2021-07-22T15:54:51Z
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