PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo

Hočevar, Sandra; Puddinu, Viola; Haeni, Laetitia; Petri-Fink, Alke; Wagner, Julia; Alvarez, Montserrat; Clift, Martin; Bourquin, Carole

doi:10.1021/acsnano.2c04871

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PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo

Sandra Hočevar

, Viola Puddinu

, Laetitia Haeni, Alke Petri-Fink

, Julia Wagner, Montserrat Alvarez, Martin Clift

, Carole Bourquin

ACS Nano, Volume: 16, Issue: 11, Pages: 18119 - 18132

Swansea University Author: Martin Clift

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DOI (Published version): 10.1021/acsnano.2c04871

Abstract

Engineered gold nanoparticles (GNPs) have become a useful tool in various therapeutic and diagnostic applications. Uncertainty remains regarding the possible impact of GNPs on the immune system. In this regard, we investigated the interactions of polymer-coated GNPs with B cells and their functions...

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Published in:	ACS Nano
ISSN:	1936-0851 1936-086X
Published:	American Chemical Society (ACS) 2022
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa61638
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spelling	v2 61638 2022-10-24 PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false 2022-10-24 BMS Engineered gold nanoparticles (GNPs) have become a useful tool in various therapeutic and diagnostic applications. Uncertainty remains regarding the possible impact of GNPs on the immune system. In this regard, we investigated the interactions of polymer-coated GNPs with B cells and their functions in mice. Surprisingly, we observed that polymer-coated GNPs mainly interact with the recently identified subpopulation of B lymphocytes named age-associated B cells (ABCs). Importantly, we also showed that GNPs did not affect cell viability or the percentages of other B cell populations in different organs. Furthermore, GNPs did not activate B cell innate-like immune responses in any of the tested conditions, nor did they impair adaptive B cell responses in immunized mice. Together, these data provide an important contribution to the otherwise limited knowledge about GNP interference with B cell immune function, and demonstrate that GNPs represent a safe tool to target ABCs in vivo for potential clinical applications. Journal Article ACS Nano 16 11 18119 18132 American Chemical Society (ACS) 1936-0851 1936-086X gold nanoparticles; polymer-coating; in vivo toxicity; targeted delivery, B cells, age-associated B cells 27 10 2022 2022-10-27 10.1021/acsnano.2c04871 PMCID# PMC9706664 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University SU Library paid the OA fee (TA Institutional Deal) Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung - 156372, 156871, 182317; Adolphe Merkle Foundation 2023-09-13T14:51:08.7616929 2022-10-24T10:36:09.7974385 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Sandra Hočevar 0000-0002-9770-8229 1 Viola Puddinu 0000-0001-8102-6429 2 Laetitia Haeni 3 Alke Petri-Fink 0000-0003-3952-7849 4 Julia Wagner 5 Montserrat Alvarez 6 Martin Clift 0000-0001-6133-3368 7 Carole Bourquin 0000-0003-3862-4583 8 61638__25754__15d597709481475d81f5dbab4386abdf.pdf 61638.pdf 2022-11-14T10:54:49.9234611 Output 5143439 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution 4.0 International (CC BY 4.0) License. true eng https://creativecommons.org/licenses/by/4.0/
title	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo
spellingShingle	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo Martin Clift
title_short	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo
title_full	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo
title_fullStr	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo
title_full_unstemmed	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo
title_sort	PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo
author_id_str_mv	71bf49b157691e541950f5c3f49c9169
author_id_fullname_str_mv	71bf49b157691e541950f5c3f49c9169_***_Martin Clift
author	Martin Clift
author2	Sandra Hočevar Viola Puddinu Laetitia Haeni Alke Petri-Fink Julia Wagner Montserrat Alvarez Martin Clift Carole Bourquin
format	Journal article
container_title	ACS Nano
container_volume	16
container_issue	11
container_start_page	18119
publishDate	2022
institution	Swansea University
issn	1936-0851 1936-086X
doi_str_mv	10.1021/acsnano.2c04871
publisher	American Chemical Society (ACS)
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description	Engineered gold nanoparticles (GNPs) have become a useful tool in various therapeutic and diagnostic applications. Uncertainty remains regarding the possible impact of GNPs on the immune system. In this regard, we investigated the interactions of polymer-coated GNPs with B cells and their functions in mice. Surprisingly, we observed that polymer-coated GNPs mainly interact with the recently identified subpopulation of B lymphocytes named age-associated B cells (ABCs). Importantly, we also showed that GNPs did not affect cell viability or the percentages of other B cell populations in different organs. Furthermore, GNPs did not activate B cell innate-like immune responses in any of the tested conditions, nor did they impair adaptive B cell responses in immunized mice. Together, these data provide an important contribution to the otherwise limited knowledge about GNP interference with B cell immune function, and demonstrate that GNPs represent a safe tool to target ABCs in vivo for potential clinical applications.
published_date	2022-10-27T14:51:10Z
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PEGylated Gold Nanoparticles Target Age-Associated B Cells In Vivo

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