E-Thesis 145 views 47 downloads
Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds / XIAO LI
Swansea University Author: XIAO LI
Abstract
As the demand for global bone graft surgery increases, there is a growing need for more effective bone graft substitutes. Currently used bone grafting materials at the clinical level show numerous limitations, highlighting the need for producible and more effective bone graft substitutes. Hydroxyapa...
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Swansea
2022
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| Institution: | Swansea University |
| Degree level: | Master of Research |
| Degree name: | MRes |
| Supervisor: | Xia, ZhiDao |
| URI: | https://cronfa.swan.ac.uk/Record/cronfa62108 |
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| Abstract: |
As the demand for global bone graft surgery increases, there is a growing need for more effective bone graft substitutes. Currently used bone grafting materials at the clinical level show numerous limitations, highlighting the need for producible and more effective bone graft substitutes. Hydroxyapatite/calcium carbonate (HA/CC) is a material similar to natural bone tissue with biodegradability and osteoconductivity, and is, therefore, an ideal replacement of bone grafting. However, the osteogenic capability of HA/CC scaffold has space for improvement. Umbilical cord mesenchymal stem cells (UCMSCs) are expected to be the ideal seed cells for bone tissue engineering due to their widely available source, convenient collection, and similar biological characteristics to bone marrow-derived mesenchymal stem cells (BMSCs), but without the ethical and supply limitations of it. With the growing interest in enhancing the osteogenic capabilities of MSCs, pharmacological approaches were being widely researched. Retinoid acid (RA) is a metabolite of vitamin A. It has been proved that RA signaling plays an important role in development, differentiation and bone metabolism. In this study, we hypothesis that by using RA we can improve osteogenesis of UCMSCs on HA/CC scaffolds. Therefore, the aim of this study is to enhance osteogenic capacity of UCMSCs on HA/CC scaffolds in vitro by supplementing RA in osteogenic medium. We assessed the attachment, proliferation/cytotoxcicity of UCMSCs on HA/CC scaffolds and the effect of RA on osteogenesis of UCMSCs in vitro. Results of Live/Dead assay indicated HA/CC scaffolds possessed high biocompatibility properties evidenced with the high survival rate of UCMSCs (>90%). After 21 days of osteogenic differentiation, the ALP activity and BMP-9 concentration of UCMSCs were increased significantly (P<0.05) by RA supplementation respect with control group. In summary, by supplementing proper dose of RA can enhance the osteogenesis capacity of UCMSCs on HA/CC scaffolds in vitro, offering a potential tissue-engineered bone graft substitute for future clinical application. |
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| Keywords: |
Enhance, osteogenic capacity, osteogenesis, human umbilical cord matrix mesenchymal stem cells (UCMSCs), Retinoic acid (RA), hydroxyapatite, calcium carbonate, scaffolds |
| College: |
Faculty of Medicine, Health and Life Sciences |