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Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds / XIAO LI

Swansea University Author: XIAO LI

Abstract

As the demand for global bone graft surgery increases, there is a growing need for more effective bone graft substitutes. Currently used bone grafting materials at the clinical level show numerous limitations, highlighting the need for producible and more effective bone graft substitutes. Hydroxyapa...

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Published: Swansea 2022
Institution: Swansea University
Degree level: Master of Research
Degree name: MRes
Supervisor: Xia, ZhiDao
URI: https://cronfa.swan.ac.uk/Record/cronfa62108
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first_indexed 2022-12-02T11:24:27Z
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spelling 2022-12-02T11:33:45.1844251 v2 62108 2022-12-02 Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds c8f408ba6083a83752d5d33f7ed8767f XIAO LI XIAO LI true false 2022-12-02 As the demand for global bone graft surgery increases, there is a growing need for more effective bone graft substitutes. Currently used bone grafting materials at the clinical level show numerous limitations, highlighting the need for producible and more effective bone graft substitutes. Hydroxyapatite/calcium carbonate (HA/CC) is a material similar to natural bone tissue with biodegradability and osteoconductivity, and is, therefore, an ideal replacement of bone grafting. However, the osteogenic capability of HA/CC scaffold has space for improvement. Umbilical cord mesenchymal stem cells (UCMSCs) are expected to be the ideal seed cells for bone tissue engineering due to their widely available source, convenient collection, and similar biological characteristics to bone marrow-derived mesenchymal stem cells (BMSCs), but without the ethical and supply limitations of it. With the growing interest in enhancing the osteogenic capabilities of MSCs, pharmacological approaches were being widely researched. Retinoid acid (RA) is a metabolite of vitamin A. It has been proved that RA signaling plays an important role in development, differentiation and bone metabolism. In this study, we hypothesis that by using RA we can improve osteogenesis of UCMSCs on HA/CC scaffolds. Therefore, the aim of this study is to enhance osteogenic capacity of UCMSCs on HA/CC scaffolds in vitro by supplementing RA in osteogenic medium. We assessed the attachment, proliferation/cytotoxcicity of UCMSCs on HA/CC scaffolds and the effect of RA on osteogenesis of UCMSCs in vitro. Results of Live/Dead assay indicated HA/CC scaffolds possessed high biocompatibility properties evidenced with the high survival rate of UCMSCs (>90%). After 21 days of osteogenic differentiation, the ALP activity and BMP-9 concentration of UCMSCs were increased significantly (P<0.05) by RA supplementation respect with control group. In summary, by supplementing proper dose of RA can enhance the osteogenesis capacity of UCMSCs on HA/CC scaffolds in vitro, offering a potential tissue-engineered bone graft substitute for future clinical application. E-Thesis Swansea Enhance, osteogenic capacity, osteogenesis, human umbilical cord matrix mesenchymal stem cells (UCMSCs), Retinoic acid (RA), hydroxyapatite, calcium carbonate, scaffolds 7 11 2022 2022-11-07 COLLEGE NANME COLLEGE CODE Swansea University Xia, ZhiDao Master of Research MRes 2022-12-02T11:33:45.1844251 2022-12-02T11:21:44.7473110 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine XIAO LI 1 62108__25988__f663798d438e40fa87f87ce051bb5779.pdf Li_Xiao_MRes_Thesis_Final_Redacted_Signature.pdf 2022-12-02T11:28:47.4903470 Output 1977575 application/pdf E-Thesis – open access true Copyright: The author, Xiao Li, 2022. true eng
title Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
spellingShingle Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
XIAO LI
title_short Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
title_full Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
title_fullStr Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
title_full_unstemmed Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
title_sort Enhancing osteogenic capabilities of human umbilical cord matrix mesenchymal stem cells on 3D-printed hydroxyapatite/calcium carbonate scaffolds
author_id_str_mv c8f408ba6083a83752d5d33f7ed8767f
author_id_fullname_str_mv c8f408ba6083a83752d5d33f7ed8767f_***_XIAO LI
author XIAO LI
author2 XIAO LI
format E-Thesis
publishDate 2022
institution Swansea University
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description As the demand for global bone graft surgery increases, there is a growing need for more effective bone graft substitutes. Currently used bone grafting materials at the clinical level show numerous limitations, highlighting the need for producible and more effective bone graft substitutes. Hydroxyapatite/calcium carbonate (HA/CC) is a material similar to natural bone tissue with biodegradability and osteoconductivity, and is, therefore, an ideal replacement of bone grafting. However, the osteogenic capability of HA/CC scaffold has space for improvement. Umbilical cord mesenchymal stem cells (UCMSCs) are expected to be the ideal seed cells for bone tissue engineering due to their widely available source, convenient collection, and similar biological characteristics to bone marrow-derived mesenchymal stem cells (BMSCs), but without the ethical and supply limitations of it. With the growing interest in enhancing the osteogenic capabilities of MSCs, pharmacological approaches were being widely researched. Retinoid acid (RA) is a metabolite of vitamin A. It has been proved that RA signaling plays an important role in development, differentiation and bone metabolism. In this study, we hypothesis that by using RA we can improve osteogenesis of UCMSCs on HA/CC scaffolds. Therefore, the aim of this study is to enhance osteogenic capacity of UCMSCs on HA/CC scaffolds in vitro by supplementing RA in osteogenic medium. We assessed the attachment, proliferation/cytotoxcicity of UCMSCs on HA/CC scaffolds and the effect of RA on osteogenesis of UCMSCs in vitro. Results of Live/Dead assay indicated HA/CC scaffolds possessed high biocompatibility properties evidenced with the high survival rate of UCMSCs (>90%). After 21 days of osteogenic differentiation, the ALP activity and BMP-9 concentration of UCMSCs were increased significantly (P<0.05) by RA supplementation respect with control group. In summary, by supplementing proper dose of RA can enhance the osteogenesis capacity of UCMSCs on HA/CC scaffolds in vitro, offering a potential tissue-engineered bone graft substitute for future clinical application.
published_date 2022-11-07T04:21:28Z
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score 11.012678

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