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E-Thesis 220 views 77 downloads

Development of an amyloid protein-based composite biomaterial for coating applications / CRAIG ALLAN

Swansea University Author: CRAIG ALLAN

DOI (Published version): 10.23889/SUthesis.63533

Abstract

Streptomyces bacteria are highly versatile micro-organisms, which have been recognised as potent biochemical, soil and biomaterials engineers. In particular, functional non-pathogenic amyloid proteins can be formed from the expression of β-sheet proteins. These β-sheet proteins known as chaplins, wh...

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Published: Swansea, Wales, UK 2023
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
Supervisor: van Keulen, Geertje. and Penney, David.
URI: https://cronfa.swan.ac.uk/Record/cronfa63533
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first_indexed 2023-05-24T13:54:45Z
last_indexed 2023-05-24T13:54:45Z
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spelling v2 63533 2023-05-24 Development of an amyloid protein-based composite biomaterial for coating applications 112949eefded5e59ac28edd7cc215ee1 CRAIG ALLAN CRAIG ALLAN true false 2023-05-24 Streptomyces bacteria are highly versatile micro-organisms, which have been recognised as potent biochemical, soil and biomaterials engineers. In particular, functional non-pathogenic amyloid proteins can be formed from the expression of β-sheet proteins. These β-sheet proteins known as chaplins, which aggregate to form a fibrillar morphology, has been shown to protect against desiccation in hydrophobic environments. These robust Chaplin proteins have served here as a source of inspiration for materials development, based on the chaplins’ ability to modulate the properties of its own surface and that of its natural environment.This study has developed more economical and environmentally friendlier methods for chaplin protein production by replacing the existing TES buffer for a potassium bicarbonate buffer and by modifying the downstream processing to assist in the removal of trifluoroacetic acid. Combinations of different media and buffers were tested for alternative fermentations that support Streptomyces morphological differentiation in liquid media, in which a potassium bicarbonate buffer system proved as efficient as well as more economical when compared to conventional fermentations with expensive organic buffer systems that support differentiation. Downstream processing of amyloid proteins was furthermore improved by adopting synthetic peptide procedures resulting in an environmentally friendlier amyloid purification method. This modified medium was also demonstrated within a bioreactor at 1.7 L scale which further enhances the economic benefit which could be implemented for production of other secondary metabolites.The resulting chaplin proteins were then applied with β-glucans to form a biocomposite for different industrial applications. Material properties and anti-corrosion were determined by goniometry and high-resolution imaging, and by qualitative and quantitative electrochemistry. Our protein-based corrosion resistant nano-coating has great potential for the manufacturing, defence and other industries, including healthcare and biomaterials manufacturing. E-Thesis Swansea, Wales, UK Streptomyces, biomaterials, corrosion, fermentation 4 4 2023 2023-04-04 10.23889/SUthesis.63533 COLLEGE NANME COLLEGE CODE Swansea University van Keulen, Geertje. and Penney, David. Doctoral Ph.D Defence Science and technology laboratory 2023-09-29T10:18:38.2306961 2023-05-24T14:48:59.5940458 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science CRAIG ALLAN 1 63533__27598__93bdad1f9fa44cd9a307d96bc34aab59.pdf 2023_Allan_C.final.63533.pdf 2023-05-24T14:55:22.5253899 Output 7334342 application/pdf E-Thesis – open access true Copyright: The Author, Craig Allan, 2023. true eng
title Development of an amyloid protein-based composite biomaterial for coating applications
spellingShingle Development of an amyloid protein-based composite biomaterial for coating applications
CRAIG ALLAN
title_short Development of an amyloid protein-based composite biomaterial for coating applications
title_full Development of an amyloid protein-based composite biomaterial for coating applications
title_fullStr Development of an amyloid protein-based composite biomaterial for coating applications
title_full_unstemmed Development of an amyloid protein-based composite biomaterial for coating applications
title_sort Development of an amyloid protein-based composite biomaterial for coating applications
author_id_str_mv 112949eefded5e59ac28edd7cc215ee1
author_id_fullname_str_mv 112949eefded5e59ac28edd7cc215ee1_***_CRAIG ALLAN
author CRAIG ALLAN
author2 CRAIG ALLAN
format E-Thesis
publishDate 2023
institution Swansea University
doi_str_mv 10.23889/SUthesis.63533
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
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description Streptomyces bacteria are highly versatile micro-organisms, which have been recognised as potent biochemical, soil and biomaterials engineers. In particular, functional non-pathogenic amyloid proteins can be formed from the expression of β-sheet proteins. These β-sheet proteins known as chaplins, which aggregate to form a fibrillar morphology, has been shown to protect against desiccation in hydrophobic environments. These robust Chaplin proteins have served here as a source of inspiration for materials development, based on the chaplins’ ability to modulate the properties of its own surface and that of its natural environment.This study has developed more economical and environmentally friendlier methods for chaplin protein production by replacing the existing TES buffer for a potassium bicarbonate buffer and by modifying the downstream processing to assist in the removal of trifluoroacetic acid. Combinations of different media and buffers were tested for alternative fermentations that support Streptomyces morphological differentiation in liquid media, in which a potassium bicarbonate buffer system proved as efficient as well as more economical when compared to conventional fermentations with expensive organic buffer systems that support differentiation. Downstream processing of amyloid proteins was furthermore improved by adopting synthetic peptide procedures resulting in an environmentally friendlier amyloid purification method. This modified medium was also demonstrated within a bioreactor at 1.7 L scale which further enhances the economic benefit which could be implemented for production of other secondary metabolites.The resulting chaplin proteins were then applied with β-glucans to form a biocomposite for different industrial applications. Material properties and anti-corrosion were determined by goniometry and high-resolution imaging, and by qualitative and quantitative electrochemistry. Our protein-based corrosion resistant nano-coating has great potential for the manufacturing, defence and other industries, including healthcare and biomaterials manufacturing.
published_date 2023-04-04T10:18:39Z
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score 10.9979105

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