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Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin

Liana Azizova Orcid Logo, Adnan Al Dalaty, Emmanuel Brousseau, James Birchall, Thomas Wilkinson Orcid Logo, Alastair Sloan Orcid Logo, Wayne Nishio Ayre

Applied Surface Science, Volume: 665, Start page: 160337

Swansea University Author: Thomas Wilkinson Orcid Logo

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DOI (Published version): 10.1016/j.apsusc.2024.160337

Abstract

Infections represent a significant challenge in joint replacements, often leading to the need for high-risk revision surgeries. There is an unmet need for novel technologies that are triggered by pathogens to prevent long-term joint replacement infections. The use of supported lipid bilayers (SLBs)...

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Published in: Applied Surface Science
ISSN: 0169-4332
Published: Elsevier BV 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa66603
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spelling v2 66603 2024-06-06 Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin 86cca6bf31bfe8572de27c1b441420d8 0000-0003-0397-6079 Thomas Wilkinson Thomas Wilkinson true false 2024-06-06 MEDS Infections represent a significant challenge in joint replacements, often leading to the need for high-risk revision surgeries. There is an unmet need for novel technologies that are triggered by pathogens to prevent long-term joint replacement infections. The use of supported lipid bilayers (SLBs) with encapsulated antimicrobial agents, which are responsive to bacterial virulence factors, offers an exciting approach to achieving this goal. In this study, Ti was functionalised using octadecylphosphonic acid (ODPA) to form an SLB with an encapsulated antibiotic (novobiocin), effective against methicillin-resistant Staphylococcus aureus. Using the solvent-assisted method, the SLB with encapsulated novobiocin was developed on the surface of ODPA-modified Ti quartz crystal microbalance (QCM) sensors. QCM monitoring and fluorescence microscopy supported the successful formation of a planar SLB with encapsulated novobiocin. Incorporation of novobiocin in the SLB resulted in significantly reduced attachment and viability of S. aureus NCTC 7791, with no significant reduction in human bone marrow stromal cell viability. Additionally, in the presence of varying concentrations of α-haemolysin, a virulence factor from S. aureus, the SLB demonstrated a dose-dependent release pattern. The findings indicate the possibility of creating a biocompatible implant coating that releases an antimicrobial in the presence of a bacterial virulence factor, in a dose-dependent manner. Journal Article Applied Surface Science 665 160337 Elsevier BV 0169-4332 Supported lipid bilayer; Novobiocin; Quartz crystal microbalance; Bacterial virulence factors; Joint replacement infection; Responsive implant coatings 30 8 2024 2024-08-30 10.1016/j.apsusc.2024.160337 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Another institution paid the OA fee This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) grant number EP/T016124/1. 2024-07-10T16:21:57.1362657 2024-06-06T09:32:37.6027259 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Liana Azizova 0000-0001-7096-4452 1 Adnan Al Dalaty 2 Emmanuel Brousseau 3 James Birchall 4 Thomas Wilkinson 0000-0003-0397-6079 5 Alastair Sloan 0000-0002-1791-0903 6 Wayne Nishio Ayre 7 66603__30563__2b5457cb28e34f5086345e1ce1f4e135.pdf 66603.pdf 2024-06-07T10:45:16.8376713 Output 3258922 application/pdf Version of Record true This is an open access article under the CC BY license. true eng https://creativecommons.org/licenses/by/4.0/
title Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
spellingShingle Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
Thomas Wilkinson
title_short Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
title_full Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
title_fullStr Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
title_full_unstemmed Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
title_sort Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
author_id_str_mv 86cca6bf31bfe8572de27c1b441420d8
author_id_fullname_str_mv 86cca6bf31bfe8572de27c1b441420d8_***_Thomas Wilkinson
author Thomas Wilkinson
author2 Liana Azizova
Adnan Al Dalaty
Emmanuel Brousseau
James Birchall
Thomas Wilkinson
Alastair Sloan
Wayne Nishio Ayre
format Journal article
container_title Applied Surface Science
container_volume 665
container_start_page 160337
publishDate 2024
institution Swansea University
issn 0169-4332
doi_str_mv 10.1016/j.apsusc.2024.160337
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
active_str 0
description Infections represent a significant challenge in joint replacements, often leading to the need for high-risk revision surgeries. There is an unmet need for novel technologies that are triggered by pathogens to prevent long-term joint replacement infections. The use of supported lipid bilayers (SLBs) with encapsulated antimicrobial agents, which are responsive to bacterial virulence factors, offers an exciting approach to achieving this goal. In this study, Ti was functionalised using octadecylphosphonic acid (ODPA) to form an SLB with an encapsulated antibiotic (novobiocin), effective against methicillin-resistant Staphylococcus aureus. Using the solvent-assisted method, the SLB with encapsulated novobiocin was developed on the surface of ODPA-modified Ti quartz crystal microbalance (QCM) sensors. QCM monitoring and fluorescence microscopy supported the successful formation of a planar SLB with encapsulated novobiocin. Incorporation of novobiocin in the SLB resulted in significantly reduced attachment and viability of S. aureus NCTC 7791, with no significant reduction in human bone marrow stromal cell viability. Additionally, in the presence of varying concentrations of α-haemolysin, a virulence factor from S. aureus, the SLB demonstrated a dose-dependent release pattern. The findings indicate the possibility of creating a biocompatible implant coating that releases an antimicrobial in the presence of a bacterial virulence factor, in a dose-dependent manner.
published_date 2024-08-30T16:21:55Z
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