Journal article 60 views 21 downloads
Antimicrobial release from a lipid bilayer titanium implant coating is triggered by Staphylococcus aureus alpha-haemolysin
Liana Azizova 
,
Adnan Al Dalaty,
Emmanuel Brousseau,
James Birchall,
Thomas Wilkinson ,
Alastair Sloan ,
Wayne Nishio Ayre
,
Adnan Al Dalaty,
Emmanuel Brousseau,
James Birchall,
Thomas Wilkinson ,
Alastair Sloan ,
Wayne Nishio Ayre
Applied Surface Science, Volume: 665, Start page: 160337
Swansea University Author:
Thomas Wilkinson
DOI (Published version): 10.1016/j.apsusc.2024.160337
Abstract
Infections represent a significant challenge in joint replacements, often leading to the need for high-risk revision surgeries. There is an unmet need for novel technologies that are triggered by pathogens to prevent long-term joint replacement infections. The use of supported lipid bilayers (SLBs)...
| Published in: | Applied Surface Science |
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| ISSN: | 0169-4332 |
| Published: |
Elsevier BV
2024
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa66603 |
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| Abstract: |
Infections represent a significant challenge in joint replacements, often leading to the need for high-risk revision surgeries. There is an unmet need for novel technologies that are triggered by pathogens to prevent long-term joint replacement infections. The use of supported lipid bilayers (SLBs) with encapsulated antimicrobial agents, which are responsive to bacterial virulence factors, offers an exciting approach to achieving this goal. In this study, Ti was functionalised using octadecylphosphonic acid (ODPA) to form an SLB with an encapsulated antibiotic (novobiocin), effective against methicillin-resistant Staphylococcus aureus. Using the solvent-assisted method, the SLB with encapsulated novobiocin was developed on the surface of ODPA-modified Ti quartz crystal microbalance (QCM) sensors. QCM monitoring and fluorescence microscopy supported the successful formation of a planar SLB with encapsulated novobiocin. Incorporation of novobiocin in the SLB resulted in significantly reduced attachment and viability of S. aureus NCTC 7791, with no significant reduction in human bone marrow stromal cell viability. Additionally, in the presence of varying concentrations of α-haemolysin, a virulence factor from S. aureus, the SLB demonstrated a dose-dependent release pattern. The findings indicate the possibility of creating a biocompatible implant coating that releases an antimicrobial in the presence of a bacterial virulence factor, in a dose-dependent manner. |
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| Keywords: |
Supported lipid bilayer; Novobiocin; Quartz crystal microbalance; Bacterial virulence factors; Joint replacement infection; Responsive implant coatings |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) grant number EP/T016124/1. |
| Start Page: |
160337 |