Journal article 95 views
Tungsten carbide-cobalt can function as a particle positive control for genotoxicity <i>in vitro</i> in specific cell lines
Michael Burgum,
Stephen Evans 
,
Ilaria Zanoni,
Magda Blosi,
Gareth Jenkins ,
shareen Doak
,
Ilaria Zanoni,
Magda Blosi,
Gareth Jenkins ,
shareen Doak
Mutagenesis
Swansea University Authors:
Michael Burgum, Stephen Evans , Gareth Jenkins , shareen Doak
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1093/mutage/geaf021
Abstract
Nanoparticle genotoxicity can be induced through several mechanisms, but there are currently no nanoparticle positive controls available for the evaluation of in vitro genotoxicity. Tungsten carbide-cobalt (WC/Co) has been proposed as one possible candidate. The aim of this study was therefore to in...
| Published in: | Mutagenesis |
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| ISSN: | 0267-8357 1464-3804 |
| Published: |
Oxford University Press (OUP)
2025
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa70577 |
| Abstract: |
Nanoparticle genotoxicity can be induced through several mechanisms, but there are currently no nanoparticle positive controls available for the evaluation of in vitro genotoxicity. Tungsten carbide-cobalt (WC/Co) has been proposed as one possible candidate. The aim of this study was therefore to investigate the genotoxic profile of WC/Co (Co 8% wt.) utilising the cytokinesis-blocked micronucleus (CBMN) assay, the mammalian cell gene mutation test, and comet assay following a 24-hour exposure. This was conducted in human lymphoblast (TK6) and Chinese hamster lung fibroblast (V79-4) cells. No cytotoxicity was observed in the TK6 CBMN assay even when significant induction of micronuclei was observed at 100 μg/ml (2-fold over control). In contrast, V79-4 cells demonstrated no significant genotoxicity or cytotoxicity in the CBMN assay. In the gene mutation assay significant mutagenicity was observed in V79-4 cells at 100 μg/ml (2-fold over control). Cellular uptake of the WC/Co nanoparticles was not qualitatively detected in either cell type when investigated with transmission electron microscopy (TEM). No genotoxicity was observed in either cell type with the comet assay. The data generated indicates that WC/Co nanoparticles may be used as a positive particulate control in the CBMN assay when using TK6 cells only; whilst in the gene mutation assay it can be used as a positive control for V79-4 cells. However, its use as a particle positive control is only possible when applying the highest test concentration of 100 μg/ml. |
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| Keywords: |
genotoxicity, in vitro, Tungsten carbide-cobalt |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
The lead author would like to thank the United Kingdom Environmental Mutagen Society (UKEMS) for awarding the Small Grants Bursary. The authors would like to acknowledge this research has received funding from the European Union’s Horizon 2020 research and innovation program for the RiskGONE project, grant agreement 814425. Stephen J Evans was supported by the Celtic Advanced Life Science Innovation Network, an Ireland-Wales 2014-2020 programme part funded by the European Regional Development Fund through the Welsh Government (80885). The authors would like to acknowledge that Magda Blosi and Ilaria Zanoni were part funded by the SUNSHINE project, grant agreement 952924. |