No Cover Image

Journal article 1037 views 127 downloads

Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics

Feng Liang, Yuzheng Guo Orcid Logo, Shaocong Hou, Qimin Quan

Science Advances, Volume: 3, Issue: 5, Start page: e1602991

Swansea University Author: Yuzheng Guo Orcid Logo

Check full text

DOI (Published version): 10.1126/sciadv.1602991

Abstract

Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-i...

Full description

Published in: Science Advances
ISSN: 2375-2548
Published: 2017
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa37809
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-in-a-nanocavity single-molecule biosensor to study DNA-protein dynamics without using fluorescent labels. Our results indicate that the fluorescein and fluorescent protein labels decrease the interaction between a single DNA and a protein due to weakened electrostatic interaction. Although the study is performed on the DNA-XPA system, the conclusion has a general implication that the traditional fluorescent labeling methods might be misestimating the molecular interactions.
Keywords: single molecule, Photonic Crystal, Plasmonics, Nanosensor, Moleulcar dynamics, Fluorescent labeling effect, Label-free
College: Faculty of Science and Engineering
Issue: 5
Start Page: e1602991