Journal article 1113 views 135 downloads
Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
Feng Liang,
Yuzheng Guo 
,
Shaocong Hou,
Qimin Quan
,
Shaocong Hou,
Qimin Quan
Science Advances, Volume: 3, Issue: 5, Start page: e1602991
Swansea University Author:
Yuzheng Guo
-
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DOI (Published version): 10.1126/sciadv.1602991
Abstract
Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-i...
| Published in: | Science Advances |
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| ISSN: | 2375-2548 |
| Published: |
2017
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa37809 |
| Abstract: |
Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-in-a-nanocavity single-molecule biosensor to study DNA-protein dynamics without using fluorescent labels. Our results indicate that the fluorescein and fluorescent protein labels decrease the interaction between a single DNA and a protein due to weakened electrostatic interaction. Although the study is performed on the DNA-XPA system, the conclusion has a general implication that the traditional fluorescent labeling methods might be misestimating the molecular interactions. |
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| Keywords: |
single molecule, Photonic Crystal, Plasmonics, Nanosensor, Moleulcar dynamics, Fluorescent labeling effect, Label-free |
| College: |
Faculty of Science and Engineering |
| Issue: |
5 |
| Start Page: |
e1602991 |