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Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics / Feng Liang; Yuzheng Guo; Shaocong Hou; Qimin Quan

Science Advances, Volume: 3, Issue: 5, Start page: e1602991

Swansea University Author: Guo, Yuzheng

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DOI (Published version): 10.1126/sciadv.1602991

Abstract

Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-i...

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Published in: Science Advances
ISSN: 2375-2548
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa37809
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spelling 2019-08-19T14:23:25Z v2 37809 2018-01-03 Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics Yuzheng Guo Yuzheng Guo true 0000-0003-2656-0340 false 2c285ab01f88f7ecb25a3aacabee52ea ca6eaec62d53427333c2fdd0967e29f3 yuiXDDPmSM3HuYmRdX7apxXCE6Z9OGBXOD9D5JU4+T4= 2018-01-03 EEN Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-in-a-nanocavity single-molecule biosensor to study DNA-protein dynamics without using fluorescent labels. Our results indicate that the fluorescein and fluorescent protein labels decrease the interaction between a single DNA and a protein due to weakened electrostatic interaction. Although the study is performed on the DNA-XPA system, the conclusion has a general implication that the traditional fluorescent labeling methods might be misestimating the molecular interactions. Journal article Science Advances 3 5 e1602991 2375-2548 single molecule, Photonic Crystal, Plasmonics, Nanosensor, Moleulcar dynamics, Fluorescent labeling effect, Label-free 26 5 2017 2017-05-26 10.1126/sciadv.1602991 College of Engineering Engineering CENG EEN None None 2019-08-19T14:23:25Z 2018-01-03T10:27:04Z College of Engineering Engineering Feng Liang 1 Yuzheng Guo 2 Shaocong Hou 3 Qimin Quan 4 0037809-03012018103001.pdf liang2017.pdf 2018-01-03T10:30:01Z Output 1189009 application/pdf VoR true Updated Copyright 27/02/2018 2018-01-03T00:00:00 true eng
title Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
spellingShingle Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
Guo, Yuzheng
title_short Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_full Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_fullStr Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_full_unstemmed Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_sort Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
author_id_str_mv 2c285ab01f88f7ecb25a3aacabee52ea
author_id_fullname_str_mv 2c285ab01f88f7ecb25a3aacabee52ea_***_Guo, Yuzheng
author Guo, Yuzheng
author2 Feng Liang
Yuzheng Guo
Shaocong Hou
Qimin Quan
format Journal article
container_title Science Advances
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container_issue 5
container_start_page e1602991
publishDate 2017
institution Swansea University
issn 2375-2548
doi_str_mv 10.1126/sciadv.1602991
college_str College of Engineering
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hierarchy_top_title College of Engineering
hierarchy_parent_id collegeofengineering
hierarchy_parent_title College of Engineering
department_str Engineering{{{_:::_}}}College of Engineering{{{_:::_}}}Engineering
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description Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-in-a-nanocavity single-molecule biosensor to study DNA-protein dynamics without using fluorescent labels. Our results indicate that the fluorescein and fluorescent protein labels decrease the interaction between a single DNA and a protein due to weakened electrostatic interaction. Although the study is performed on the DNA-XPA system, the conclusion has a general implication that the traditional fluorescent labeling methods might be misestimating the molecular interactions.
published_date 2017-05-26T21:54:51Z
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