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Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics

Feng Liang, Yuzheng Guo Orcid Logo, Shaocong Hou, Qimin Quan

Science Advances, Volume: 3, Issue: 5, Start page: e1602991

Swansea University Author: Yuzheng Guo Orcid Logo

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DOI (Published version): 10.1126/sciadv.1602991

Abstract

Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-i...

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Published in: Science Advances
ISSN: 2375-2548
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa37809
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spelling 2021-09-24T14:58:16.7317021 v2 37809 2018-01-03 Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics 2c285ab01f88f7ecb25a3aacabee52ea 0000-0003-2656-0340 Yuzheng Guo Yuzheng Guo true false 2018-01-03 ACEM Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-in-a-nanocavity single-molecule biosensor to study DNA-protein dynamics without using fluorescent labels. Our results indicate that the fluorescein and fluorescent protein labels decrease the interaction between a single DNA and a protein due to weakened electrostatic interaction. Although the study is performed on the DNA-XPA system, the conclusion has a general implication that the traditional fluorescent labeling methods might be misestimating the molecular interactions. Journal Article Science Advances 3 5 e1602991 2375-2548 single molecule, Photonic Crystal, Plasmonics, Nanosensor, Moleulcar dynamics, Fluorescent labeling effect, Label-free 26 5 2017 2017-05-26 10.1126/sciadv.1602991 COLLEGE NANME Aerospace, Civil, Electrical, and Mechanical Engineering COLLEGE CODE ACEM Swansea University 2021-09-24T14:58:16.7317021 2018-01-03T10:27:04.8056805 Faculty of Science and Engineering School of Aerospace, Civil, Electrical, General and Mechanical Engineering - General Engineering Feng Liang 1 Yuzheng Guo 0000-0003-2656-0340 2 Shaocong Hou 3 Qimin Quan 4 0037809-03012018103001.pdf liang2017.pdf 2018-01-03T10:30:01.8070000 Output 1189009 application/pdf Version of Record true 2018-01-03T00:00:00.0000000 true eng
title Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
spellingShingle Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
Yuzheng Guo
title_short Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_full Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_fullStr Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_full_unstemmed Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
title_sort Photonic-plasmonic hybrid single-molecule nanosensor measures the effect of fluorescent labels on DNA-protein dynamics
author_id_str_mv 2c285ab01f88f7ecb25a3aacabee52ea
author_id_fullname_str_mv 2c285ab01f88f7ecb25a3aacabee52ea_***_Yuzheng Guo
author Yuzheng Guo
author2 Feng Liang
Yuzheng Guo
Shaocong Hou
Qimin Quan
format Journal article
container_title Science Advances
container_volume 3
container_issue 5
container_start_page e1602991
publishDate 2017
institution Swansea University
issn 2375-2548
doi_str_mv 10.1126/sciadv.1602991
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Aerospace, Civil, Electrical, General and Mechanical Engineering - General Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Aerospace, Civil, Electrical, General and Mechanical Engineering - General Engineering
document_store_str 1
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description Current methods to study molecular interactions require labeling the subject molecules with fluorescent reporters. However, the effect of the fluorescent reporters on molecular dynamics has not been quantified because of a lack of alternative methods. We develop a hybrid photonic-plasmonic antenna-in-a-nanocavity single-molecule biosensor to study DNA-protein dynamics without using fluorescent labels. Our results indicate that the fluorescein and fluorescent protein labels decrease the interaction between a single DNA and a protein due to weakened electrostatic interaction. Although the study is performed on the DNA-XPA system, the conclusion has a general implication that the traditional fluorescent labeling methods might be misestimating the molecular interactions.
published_date 2017-05-26T04:19:55Z
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score 11.390808

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