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Sterolomics in biology, biochemistry, medicine / William J. Griffiths; Yuqin Wang

TrAC Trends in Analytical Chemistry

Swansea University Author: Griffiths, William

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Abstract

In mammalian systems “sterolomics” can be regarded as the quantitative or semi-quantitative profiling of all metabolites derived from cholesterol and its cyclic precursors. The system can be further complicated by metabolites derived from ingested phytosterols or pharmaceuticals, but this is beyond...

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Published in: TrAC Trends in Analytical Chemistry
ISSN: 01659936
Published: 2018
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa45237
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Abstract: In mammalian systems “sterolomics” can be regarded as the quantitative or semi-quantitative profiling of all metabolites derived from cholesterol and its cyclic precursors. The system can be further complicated by metabolites derived from ingested phytosterols or pharmaceuticals, but this is beyond the scope of this article. “Sterolomics” can be performed on either an unbiased global format, or more usually, exploiting a targeted format. Here we discuss the different mass spectrometry-based analytical techniques used in “sterolomics” giving specific examples in the context of neurodegenerative disease and for the diagnosis of inborn errors of metabolism. We pay particular attention to the profiling of cholesterol metabolites in the bile acid biosynthesis pathways, although the analytical techniques discussed are also appropriate for analysis of hormonal steroids.
Keywords: Mass spectrometry, liquid chromatography – mass spectrometry, gas chromatography – mass spectrometry, Alzheimer’s disease, Niemann Pick disease, inborn errors of metabolism, bile acids, oxysterols
College: Swansea University Medical School